Development of Rapid One-Step Immunochromatographic Assay

Paek, Se‐Hwan; Lee, Seung Hwa; Cho, Joung-Hwan; Kim, Young-Sang

doi:10.1006/meth.2000.1036

Cited by 189 publications

(124 citation statements)

References 40 publications

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“…The results obtained from the study of 847 CSF samples from meningitis suspected patients (Table 4) For the new RDTs, CSF samples can be tested without prior heat or centrifugation, as opposed to the LAT. In addition, these tests are equally reliable at 25 and 45 • C ambient temperature, and the RDT performance is resistant to exposure for 3 weeks to 60 • C (simulating 2 years storage at 25 • C) [26,27]. These tests can therefore be used without the need for expensive or sophisticated machinery, they do not require cold chain or electricity, avoid the need for transportation of samples to reference centres and also obviate the need for highly skilled technical staff.…”

Section: The New Rapid Diagnostic Tests (Rdts)mentioning

confidence: 99%

Biological diagnosis of meningococcal meningitis in the African meningitis belt: Current epidemic strategy and new perspectives

Chanteau

Rose²,

Djibo

et al. 2007

Vaccine

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Section: The New Rapid Diagnostic Tests (Rdts)mentioning

confidence: 99%

Biological diagnosis of meningococcal meningitis in the African meningitis belt: Current epidemic strategy and new perspectives

Chanteau

Rose²,

Djibo

et al. 2007

Vaccine

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“…[2][3][4][5][6][7][8][9][10][11][12][13][14][15] Some of these tests analyze environmental conditions, 16,17 or detect illness (often in the developing world). [18][19][20] We have recently patterned paper into hydrophilic regions demarcated by hydrophobic walls using photolithography and conventional photoresists (SU-8; and poly(methyl methacrylate), or PMMA).…”

Section: Introductionmentioning

confidence: 99%

Low-Cost Printing of Poly(dimethylsiloxane) Barriers To Define Microchannels in Paper

2008

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This paper describes the use of a modified x,y-plotter to generate hydrophilic channels by printing a solution of hydrophobic polymer (poly(dimethyl siloxane; PDMS) dissolved in hexanes onto filter paper. The PDMS penetrates the depth of the paper, and forms a hydrophobic wall that aqueous solutions cannot cross. The minimum size of printed features is approximately 1 mm; this resolution is adequate for the rapid prototyping of hand-held, visually read, diagnostic assays (and other microfluidic systems) based on paper. After curing the printed PDMS, the paper-based devices can be bent or folded to generate three-dimensional (3D) systems of channels. Capillary action pulls aqueous samples into the paper channels. Colorimetric assays for the presence of glucose and protein are demonstrated in the printed devices; spots of Bromothymol Blue distinguished samples with slightly basic pH (6.5) from samples with slightly acidic pH (8.0). The work also describes using printed devices that can be loaded using multipipettes, and printed flexible, foldable channels in paper over areas larger than 100 cm 2 . This paper describes the use of a modified desktop plotter to fabricate simple patterns of hydrophilic microchannels in paper. We defined the boundaries of the microchannels by printing a solution of poly(dimethyl siloxane) (PDMS) in hexanes onto filter paper, using an x,y-plotter as a print-engine. Because PDMS is an elastomer, the paper could be bent and folded, without destroying the integrity of the channels. The channels have a minimum width of 1 mm, and the minimum spacing between two channels can be as small as 1 mm. These dimensions are large relative to those normally encountered in microfluidic systems, but they are the right size for the basic analytical and diagnostic devices that are our primary objectives, since readout of these devices will often involve visual observations of unmagnified spots of analytes. 1 We believe that this low-cost system for printing hydrophobic polymers and other materials on paper will be useful for prototyping simple paper-based diagnostics assays.A variety of simple diagnostic tests (for example, "dip-stick" tests) rely on paper-based assays. [2][3][4][5][6][7][8][9][10][11][12][13][14][15] Some of these tests analyze environmental conditions, 16,17 or detect illness (often in the developing world). [18][19][20] We have recently patterned paper into hydrophilic regions demarcated by hydrophobic walls using photolithography and conventional photoresists (SU-8; and poly(methyl methacrylate), or PMMA). 1 The hydrophilic regions act as microfluidic channels, in which capillary action wicks aqueous samples into the device. This design provides the basis for diagnostic systems in paper that are more complex than the simplest dip-stick assays (in the sense that multiple assays can be performed on a small array with ~5-20 μL of blood, urine, or other fluid), but simpler and more affordable than highertechnology microfluidic assays. 20 An advantage of using PDMS to pattern the p...

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“…O uso de enzimas como marcadores ainda é a melhor alternativa, porém algumas enzimas são extremamente caras, o que torna o método economicamente inviável. A eliminação do uso de marcadores tem sido possível, com sucesso, através do uso da imunocromatografia 169 , mas a hifenação desta técnica com FIA, sem dúvida, possibilitará resultados mais rápidos e reprodutíveis 170 .…”

Section: Avanços E Perspectivasunclassified

Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas

Nunes¹

2005

Quím. Nova

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Recebido em 6/2/04; aceito em 20/10/04; publicado na web em 28/2/05 IMMUNOCHEMICAL METHODS FOR ANALYSIS OF ENVIRONMENTAL CONTAMINANTS: CONCEPTS, STATE OF THE ART AND PERPECTIVES. Immunoassay techniques provide simple, powerful and inexpensive methods for analysis of environmental contaminants. However, the acceptance of immunoassays is dependent on the clear demonstration of quality and validity compared to more traditional techniques. In this review, primarily, the understanding and the fundamentals of immunoassay methods are given in order to make good use of immunoassays, especially of EIA tests. Special attention is given to the concepts related to the enzymelinked immunosorbent assay (ELISA) formats, such as inhibition concentration at 50% (IC 50 ), detection limit (LOD), cross-reactivity (CR %). It is also explained why some immunoassays are quantitative methods whereas others can only be used as screening methods. A list of main commercial kits for detection of priority pollutants is given in order to help analysts. Others formats, such as flowinjection immunoassay analysis (FIIA), immunoassay chromatography and immunosensors are also cited.Keywords: immunoassays; environmental contaminants; monitoring. INTRODUÇÃOA origem dos métodos imunológicos situa-se no final da déca-da de 50, quando Yalow e Berson 1 descreveram o primeiro ensaio imunológico para detectar insulina humana, o que lhes rendeu o Prêmio Nobel em 1977. A partir de então, as técnicas imunoquí-micas foram desenvolvidas como métodos de análise de macromoléculas (enzimas, hormônios), e posteriormente para moléculas orgânicas pequenas (drogas, fármacos), tendo seu maior êxito no campo da química clínica 2-4 . Em meados da década de 70, foi desenvolvido o primeiro ensaio biológico para pesticidas (aldrin e dieldrin) 5 . A este ensaio, seguiram-se outros, também baseados na marcação radioativa, chamados ensaios radioimunológicos ("radioimmunoassay, RIA"). Os ensaios imunoenzimáticos ("enzyme immunoassay, EIA") surgiram na década de 60 para identificação e localização de antígenos em preparações histológicas 2,6,7 , tendo sido a sua utilidade reconhecida posteriormente em outros campos distintos [8][9][10][11][12][13] . No início dos anos 80, começou-se a discutir as possibilidades que estes tipos de métodos analíticos podiam oferecer na detecção de contaminantes, tanto em amostras ambientais como de alimentos 12,14-16 , o que fomentou a sua aplicação no campo da química ambiental. A partir desse momento, começou-se a utilizar a marcação enzimática, que proporcionava menores riscos e apresentava sensibilidade similar aos métodos convencionais de análise 12,[15][16][17][18][19] . Inúmeras vantagens podem ser obtidas com a utilização dos métodos imunoquímicos e, paulatinamente, vão sendo disponibilizadas informações sobre diversos aspectos analíticos, tais como i) interpretação dos dados obtidos no desenvolvimento dos métodos; ii) estratégias utilizadas para a garantia da qualidade dos procedimentos operacionais; iii) análise dos dados e das informa...

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Development of Rapid One-Step Immunochromatographic Assay

Cited by 189 publications

References 40 publications

Biological diagnosis of meningococcal meningitis in the African meningitis belt: Current epidemic strategy and new perspectives

Biological diagnosis of meningococcal meningitis in the African meningitis belt: Current epidemic strategy and new perspectives

Low-Cost Printing of Poly(dimethylsiloxane) Barriers To Define Microchannels in Paper

Métodos imunoquímicos para análise de contaminantes ambientais: conceitos, estado da arte e perspectivas

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