Development of rapid on-site detection of Salmonella Enteritidis, S. Typhimurium, and S. Thompson in food samples using an ultrafast PCR system

Kim, Eiseul; Yang, Seung-Min; Kim, Hae-Yeong

doi:10.1016/j.fbio.2023.103242

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…Foodborne illnesses are a significant global public health concern , and pose a substantial threat to both national public health and economic development. , Salmonella , a Gram-negative anaerobic bacterium, is a common cause of foodborne tract outbreaks and particularly affects the safety of dairy and meat products. − Establishing rapid, efficient, and sensitive detection techniques for Salmonella is essential for effective monitoring and prompt diagnosis of its residues.…”

Section: Introductionmentioning

confidence: 99%

An Innovative and Efficient Fluorescent Detection Technique for Salmonella in Animal-Derived Foods Using the CRISPR/Cas12a-HCR System Combined with PCR/RAA

Wang,

Du,

Shao

et al. 2024

J. Agric. Food Chem.

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Here, we present a method for Salmonella detection using clustered regularly interspaced short palindromic repeats associated with the CRISPR-associated protein 12a-hybridization chain reaction (CRISPR/Cas12a-HCR) system combined with polymerase chain reaction/recombinase-assisted amplification (PCR/RAA) technology. The approach relies on the Salmonella invA gene as a biorecognition element and its amplification through PCR and RAA. In the presence of the target gene, Cas12a, guided by crRNA, recognizes and cleaves the amplification product, initiating the HCR. Fluorescently labeled single-stranded DNA (ssDNA) H1 and H2 were introduced, and the Salmonella concentration was determined based on the fluorescence intensity from the triggered HCR. Both assays demonstrate high specificity, sensitivity, simplicity, and rapidity. The detection range was 2 × 10 1 −2 × 10 9 CFU/mL, with an LOD of 20 CFU/mL, and the entire process enabled specific and rapid Salmonella detection within 85−105 min. Field-incurred spiked recovery tests were conducted in mutton and beef samples using both assays, demonstrating satisfactory recovery and accuracy in animal-derived foods. By combining CRISPR/Cas12a with hybridization chain reaction technology, this study presents a rapid and sensitive Salmonella detection method that is crucial for identifying pathogenic bacteria and monitoring food safety.

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Section: Introductionmentioning

confidence: 99%

An Innovative and Efficient Fluorescent Detection Technique for Salmonella in Animal-Derived Foods Using the CRISPR/Cas12a-HCR System Combined with PCR/RAA

Wang,

Du,

Shao

et al. 2024

J. Agric. Food Chem.

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“…Current methods for S. typhimurium detection mainly include the traditional plate culture method, 4 enzyme-linked immunosorbent assays, 5,6 and polymerase chain reaction. 7–10 Despite their widespread adoption, these methods suffer from disadvantages, such as being time-consuming, 11 and in certain contexts, compromised accuracy. 12 Consequently, there is an imperative need to construct more convenient, easy-to-operate, and accurate detection methods for S. typhimurium detection.…”

Section: Introductionmentioning

confidence: 99%

Highly sensitive detection of Salmonella typhimurium via gold and magnetic nanoparticle-mediated sandwich hybridization coupled with ICP-MS

Zhou,

Tang,

et al. 2024

J. Mater. Chem. B

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“…In comparison to antibodies, aptamers offer numerous distinctive advantages, including exceptional chemical stability, cost-effectiveness, facile synthesis and modification, and batch-to-batch consistency [11]. These superior advantages enable the extensive application of aptamers in the detection of Salmonella [12,13]. However, aptamers remain challenged in the bacterial detection of real samples due to the diminished affinity induced by food matrixes [14].…”

Section: Introductionmentioning

confidence: 99%

A Colorimetric/Fluorescent Dual-Mode Aptasensor for Salmonella Based on the Magnetic Separation of Aptamers and a DNA-Nanotriangle Programmed Multivalent Aptamer

Ma,

Sun,

Shi

et al. 2023

Foods

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Salmonella infection has emerged as a global health threat, causing death, disability, and socioeconomic disruption worldwide. The rapid and sensitive detection of Salmonella is of great significance in guaranteeing food safety. Herein, we developed a colorimetric/fluorescent dual-mode method based on a DNA-nanotriangle programmed multivalent aptamer for the sensitive detection of Salmonella. In this system, aptamers are precisely controlled and assembled on a DNA nanotriangle structure to fabricate a multivalent aptamer (NTri-Multi-Apt) with enhanced binding affinity and specificity toward Salmonella. The NTri-Multi-Apt was designed to carry many streptavidin-HRPs for colorimetric read-outs and a large load of Sybr green I in the dsDNA scaffold for the output of a fluorescent signal. Therefore, combined with the magnetic separation of aptamers and the prefabricated NTri-Multi-Apt, the dual-mode approach achieved simple and sensitive detection, with LODs of 316 and 60 CFU/mL for colorimetric and fluorescent detection, respectively. Notably, the fluorescent mode provided a self-calibrated and fivefold-improved sensitivity over colorimetric detection. Systematic results also revealed that the proposed dual-mode method exhibited high specificity and applicability for milk, egg white, and chicken meat samples, serving as a promising tool for real bacterial sample testing. As a result, the innovative dual-mode detection method showed new insights for the detection of other pathogens.

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Development of rapid on-site detection of Salmonella Enteritidis, S. Typhimurium, and S. Thompson in food samples using an ultrafast PCR system

Cited by 5 publications

References 33 publications

An Innovative and Efficient Fluorescent Detection Technique for Salmonella in Animal-Derived Foods Using the CRISPR/Cas12a-HCR System Combined with PCR/RAA

An Innovative and Efficient Fluorescent Detection Technique for Salmonella in Animal-Derived Foods Using the CRISPR/Cas12a-HCR System Combined with PCR/RAA

Highly sensitive detection of Salmonella typhimurium via gold and magnetic nanoparticle-mediated sandwich hybridization coupled with ICP-MS

A Colorimetric/Fluorescent Dual-Mode Aptasensor for Salmonella Based on the Magnetic Separation of Aptamers and a DNA-Nanotriangle Programmed Multivalent Aptamer

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