Development of radioactive tracing coupled with LC/MS-IT-TOF methodology for the discovery and identification of diaveridine metabolites in pigs

Wang, Liye; Wen, Lihua; Pan, Yuanhu; Liu, Zhenli; Zhang, Chenyun; Zhang, Yuan; Huang, Lingli

doi:10.1016/j.foodchem.2021.130200

Cited by 5 publications

(13 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The radioactive chromatographic peak showed similar characteristics as D1 in our previous studies (Figure S3). 23 This confirmed that M1 was 3′-demethyl-DVD (5-…”

Section: Excretion and Massmentioning

confidence: 72%

“…The radioactive chromatographic peak showed similar characteristics as D1 in our previous studies (Figure S3). This confirmed that M1 was 3′-demethyl-DVD (5-((2,4-diaminopyrimidin-5-yl)methyl)-2-methoxyphenol) or 4′-demethyl-DVD (4-((2,6-diaminopyridin-3-yl)methyl)-2-methoxyphenol).…”

Section: Resultsmentioning

confidence: 94%

“…DVD-related metabolites in urine, feces, plasma, bile, and major tissue samples (muscle, fat, liver, and kidneys) were extracted using optimized procedures from our previous study . Therein, an MCX-based solid-phase extraction (MCX-SPE) procedure was performed to purify liquid samples (bile, plasma, and urine).…”

Section: Methodsmentioning

confidence: 99%

“…S1; chemical purity, ≥98%; radiochemical purity, ≥98.2%; specific radioactivity, 22.08 Ci/g) was obtained according to our previous experiments. 23 This finished product was kept well at −20 °C for a full 6 months. Wuhan Yuancheng Science and Technology Development Co., Ltd. supplied the DVD with a purity level of more than 98% (Wuhan, China).…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Metabolism and Tissue Elimination of Diaveridine in Swine, Chickens, and Rats Using Radioactive Tracing Coupled with LC–ESI–IT–TOF/MS

Wang

Wen

Pan

et al. 2023

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Diaveridine (DVD) has widespread use in food animals due to its antibacterial synergistic effects. This study revealed the metabolism, excretion, and tissue elimination of DVD in swine, chickens, and rats following oral gavage of 10 mg/kg b.w. tritium-labeled DVD using radioactive tracing coupled with liquid chromatography–electron spray ionization–ion trap–time-of-flight–mass spectrometry (LC–ESI–IT–TOF/MS). The metabolic pathways involved demethylation, α-hydroxylation, glucuronidation, and sulfonylation and produced four metabolites in swine (M0, DVD; M1, 3’/4′-demethyl-DVD; M2, 3’/4′-demethyl-DVD-O-glucuronide; M4, 2/4-glucuronidated-DVD) and five in chickens (M0∼M2; M3, α-hydroxy-DVD; M4) and rats (M0∼M3; M5, 3’/4′-demethyl-DVD-O-sulfation). M0 was dominant in the excreta of chicken and female and male rats, while M2 was mainly excreted in swine. Among the three species studied, M0 was the most persistent in the kidneys (t 1/2 3.15–3.89 d); therefore, M0 kidney levels are residue monitoring targets. This study enabled a thorough comprehension of the metabolism and pharmacokinetic characteristics of DVD in animals.

show abstract

“…The radioactive chromatographic peak showed similar characteristics as D1 in our previous studies (Figure S3). 23 This confirmed that M1 was 3′-demethyl-DVD (5-…”

Section: Excretion and Massmentioning

confidence: 72%

Section: Resultsmentioning

confidence: 94%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Metabolism and Tissue Elimination of Diaveridine in Swine, Chickens, and Rats Using Radioactive Tracing Coupled with LC–ESI–IT–TOF/MS

Wang

Wen

Pan

et al. 2023

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Our previous study identified DVD metabolites in pigs at 6 h after administration by coupling radiotracing with a LC/MS-IT-TOF method. The results showed that the method was sensitive and reliable, and a total of four radiolabeled compounds were identified from this in vivo experiment, of which two were new metabolites ( 14 ). Recently, a comparative pharmacokinetic study of DVD in pigs and chickens was reported following a single intravenous or oral administration ( 15 ).…”

Section: Introductionmentioning

confidence: 89%

Metabolite Identification and Pharmacokinetic Behavior of Diaveridine in the Plasma of Pigs and Chickens Based on Radioactive Tracing Coupled With LC/MS-IT-TOF Assay

et al. 2022

Self Cite

View full text Add to dashboard Cite

Diaveridine (DVD) is widely used for the prevention and treatment of coccidiosis and leucocytozoonosis infections in food-producing animals. To gain a better understanding of DVD metabolism and pharmacokinetics in healthy Landrace/Doric Cross castrated male pigs and both female and male Cobb 500 broiler chickens, a method involving radioactive tracing coupled with LC/MS-IT-TOF was developed for the identification and quantitation of DVD and its metabolites in pig and chicken plasma, and then was applied to investigate DVD pharmacokinetics. A simple MCX solid phase extraction procedure was adopted for sample preparation. After a single oral administration of 3H-DVD (10 mg/kg BW), three radioactive compounds (D0: DVD; D1: 3′-desmethyl-DVD; and D2: monoglucuronide of 3′-desmethyl-DVD) were identified in pig plasma, while only two radioactive compounds (D0 and D2) were identified in chicken plasma. In both species, the Cmax values for all detected compounds were reached at 2 h after dosing. The Cmax order was D2 (1.38 μg/ml) > D0 (0.49 μg/ml) > D1 (0.24 μg/ml) in pigs and D0 (1.55 μg/ml) > D2 (0.27 μg/ml) in chickens. The longer t1/2 (elimination half-life) of D0 contributed to the slow elimination of DVD-related compounds. The t1/2β of D0 in pigs (66.41 h) was significantly longer than that in chickens (48.30 h), but the t1/2 of total DVD-related metabolites in pigs (42.86 h) was lower than that in chickens (56.11 h). These findings suggested that the metabolism and pharmacokinetics of DVD in pigs and chickens were significantly different, and that this would affect its effectiveness, toxicology, and food safety in these animals.

show abstract

The Impact of Ultrasonication on SDS-stabilized Silver Nanoparticles as a Probe for Trace Determination of Diaveridine

Kassem

El-Feky

2022

Plasmonics

View full text Add to dashboard Cite

Development of radioactive tracing coupled with LC/MS-IT-TOF methodology for the discovery and identification of diaveridine metabolites in pigs

Cited by 5 publications

References 29 publications

Metabolism and Tissue Elimination of Diaveridine in Swine, Chickens, and Rats Using Radioactive Tracing Coupled with LC–ESI–IT–TOF/MS

Metabolism and Tissue Elimination of Diaveridine in Swine, Chickens, and Rats Using Radioactive Tracing Coupled with LC–ESI–IT–TOF/MS

Metabolite Identification and Pharmacokinetic Behavior of Diaveridine in the Plasma of Pigs and Chickens Based on Radioactive Tracing Coupled With LC/MS-IT-TOF Assay

The Impact of Ultrasonication on SDS-stabilized Silver Nanoparticles as a Probe for Trace Determination of Diaveridine

Contact Info

Product

Resources

About