Development of QuEChERS extraction method for the determination of pesticide residues in cereals using DART-ToF-MS and GC-MS techniques. Correlation and quantification study

Hakami, Rabab A.; Aqel, Ahmad; Ghfar, Ayman A.; ALOthman, Zeid A.; Badjah-Hadj-Ahmed, Ahmed-Yacine

doi:10.1016/j.jfca.2021.103822

Cited by 16 publications

(4 citation statements)

References 33 publications

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“…18 In addition, their residue occurrence was reported around the globe, for example, in wheat from China 19 as well as in Nigerian foodstuff, 20 highlighting the need to continuously monitor them. These facts in combination with the limited number of published analytical methods for complementary determination of OP and CM pesticide residues in cereal matrices 21,22 prompted us to develop an UHPLC-QqQ-MS method for the detection of 97 OP and CM pesticide residues (see the list of analytes in ESI Table S2 †) in wheat our, a widely consumed commodity. Fit-for-purpose results were acquired and method trueness was conrmed through participating in interlaboratory PT schemes.…”

Section: Resultsmentioning

confidence: 99%

A critical comparison between an ultra-high-performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-QqQ-MS) method and an enzyme assay for anti-cholinesterase pesticide residue detection in cereal matrices

et al. 2022

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Section: Resultsmentioning

confidence: 99%

A critical comparison between an ultra-high-performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-QqQ-MS) method and an enzyme assay for anti-cholinesterase pesticide residue detection in cereal matrices

et al. 2022

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“…These acceptable CV% values were achieved by utilizing the QuEChERS clean-up to minimize the notorious matrix effect associated with the DART-HRMS technique [extensively described by Gross et al (2014)]. Although several studies applied QuEChERS as a sample clean-up prior to HPLC analysis of complex matrices, to the best of our knowledge, only two studies have combined QuEChERS with DART-HRMS (Hakami et al, 2021;Martínez-Villalba et al, 2013). Through use of the integrated QuEChERS-DART-HRMS approach described in our current study, anywhere between 20 samples can be extracted, purified, and analyzed by DART-HRMS (in triplicate) in 2 h, with minimal training needed for user to become proficient.…”

Section: Discussionmentioning

confidence: 99%

Rapid, novel screening of toxicants in poison baits, and autopsy specimens by ambient mass spectrometry

et al. 2022

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Animal poisoning and dissemination of baits in the environment have public health and ethological implications, which can be followed by criminal sanctions for those responsible. The reference methods for the analysis of suspect baits and autopsy specimens are founded on chromatographic-based techniques. They are extremely robust and sensitive, but also very expensive and laborious. For this reason, we developed an ambient mass spectrometry (AMS) method able to screen for 40 toxicants including carbamates, organophosphate and chlorinated pesticides, coumarins, metaldehyde, and strychnine. Spiked samples were firstly purified and extracted by dispersive solid phase extraction (QuEChERS) and then analyzed by direct analysis in real time high-resolution mass spectrometry (DART-HRMS). To verify the performance of this new approach, 115 authentic baits (n = 59) and necropsy specimens (gastrointestinal content and liver, n = 56) were assessed by the official reference methods and combined QuEChERS-DART-HRMS. The agreement between the results allowed evaluation of the performances of the new screening method for a variety of analytes and calculation of the resultant statistical indicators (the new method had overall accuracy 89.57%, sensitivity of 88.24%, and a specificity of 91.49%). Taking into account only the baits, 96.61% of overall accuracy was achieved with 57/59 samples correctly identified (statistical sensitivity 97.50%, statistical specificity 94.74%). Successful identification of the bitter compound, denatonium benzoate, in all the samples that contained rodenticides (28/28) was also achieved. We believe initial screening of suspect poison baits could guide the choice of reference confirmatory methods, reduce the load in official laboratories, and help the early stages of investigations into cases of animal poisoning.

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“…Due to its excellent performance, the QuE-ChERS method has become the preferred method for the pretreatment of cereal samples in Chinese national standard GB 23200.113-2018. Although rice is the most commonly food in the world, there are relatively few reports on the detection of pesticide residues in it. High performance liquid chromatography (HPLC), liquid chromatography mass spectrometry (LC-MS), gas chromatography (GC) (Uddin et al, 2011), and gas chromatography-mass spectrometry (GC-MS) (Hakami et al, 2021;Han et al, 2017) are common analytical techniques. GC and HPLC mainly rely on retention time for qualitative analysis, the screening ability of multipesticide residues is limited, and it is easy to be interfered by samples with complex matrix.…”

Section: Introductionmentioning

confidence: 99%

Efficient determination of pesticides in rice by QuEChERS and UV spectroscopy in combination with chemometrics

Wan

Jia

et al. 2023

Cereal Chem

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Background and ObjectivesTargeting the problems, like various residual pesticides, the high interference of sample matrix and the slow detection speed, this work studied the rapid screening method of multipesticide residues in rice by taking bensulfuron‐methyl, propanil and cypermethrin as examples.FindingsRice matrix solution was obtained by using the improved QuEChERS method, matrix standard solution, and solvent standard solution were prepared to obtain the linear range and matrix effect of each pesticide. QuEChERS method could rapidly pretreat samples, and the matrix effect was soft, with matrix effect values lower than 16%. Mixed solutions of the three pesticides in four combinations were prepared within the linear range. The type of pesticides in rice was determined by support vector machine (SVM), and the pesticide concentration was predicted by partial least square regression (PLSR). SVM provided a supervised identification method for pesticide residues, making the recognition accuracy higher than 90%. The error of PLSR in predicting pesticide concentration was lower than 5.36%.ConclusionsUV spectroscopy had fast test speed, so the combination of QuEChERS and UV spectroscopy could improve the efficiency of analysis. SVM combined with PLSR could preliminarily determine the type of pesticides in rice sample, and then accurately predict the concentration of pesticides.Significance and NoveltyThe QuEChERS sample pretreatment technology and UV spectroscopy in combination with chemometrics was expected to be a new technology for rapid detection of pesticide residues in samples.

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Development of QuEChERS extraction method for the determination of pesticide residues in cereals using DART-ToF-MS and GC-MS techniques. Correlation and quantification study

Cited by 16 publications

References 33 publications

A critical comparison between an ultra-high-performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-QqQ-MS) method and an enzyme assay for anti-cholinesterase pesticide residue detection in cereal matrices

A critical comparison between an ultra-high-performance liquid chromatography triple quadrupole mass spectrometry (UHPLC-QqQ-MS) method and an enzyme assay for anti-cholinesterase pesticide residue detection in cereal matrices

Rapid, novel screening of toxicants in poison baits, and autopsy specimens by ambient mass spectrometry

Efficient determination of pesticides in rice by QuEChERS and UV spectroscopy in combination with chemometrics

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