Development of quantitative plasma N-glycoproteomics using label-free 2-D LC-MALDI MS and its applicability for biomarker discovery in hepatocellular carcinoma

Ishihara, Takeshi; Fukuda, Isao; Morita, Atsushi; Takinami, Yoshihiko; Okamoto, Hiroyuki; Nishimura, Shin‐Ichiro; Numata, Yoshito

doi:10.1016/j.jprot.2011.06.010

Cited by 40 publications

(36 citation statements)

References 42 publications

(54 reference statements)

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“…Recent developments in MS technology have fueled highthroughput analyses of glycoproteins (27,28). Through the use of MS technology, thousands of new formerly N-linked glycopeptides from different tissues, cells, and bodily fluids have been identified and quantified (29,30).…”

Section: Discussionmentioning

confidence: 99%

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Modification of Sialylation Mediates the Invasive Properties and Chemosensitivity of Human Hepatocellular Carcinoma

Zhao

et al. 2014

Molecular & Cellular Proteomics

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Section: Discussionmentioning

confidence: 99%

“…Peaks 9 and 27 were exclusively detected only in the high-metastasis MHCC97H cell line. Also, peaks 12,14,16,18,19,22,28,31, and 33 were detected more frequently in MHCC97H cells (Ն2-fold). Peaks 26 and 29 presented more frequently in the MHCC97L cell line (Ն2-fold).…”

Section: Table I Qrt-pcr Conditions and Primer Sequences For Analysismentioning

confidence: 96%

Modification of Sialylation Mediates the Invasive Properties and Chemosensitivity of Human Hepatocellular Carcinoma

Zhao

et al. 2014

Molecular & Cellular Proteomics

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“…21,22 N-glycosylated proteins are also important for disease diagnosis, prognosis, and therapeutic response to drugs. 23 Various methods have been developed for in-depth characterization of the N-glycoproteome, such as hydrazide 24,25 filter aided sample preparation for mouse plasma and other organs, 23 and so on. However, usually large amount of staring material is required for these methods, which limits their application for analysis of minute amount of samples, such as clinical samples.…”

mentioning

confidence: 99%

High-Sensitivity N-Glycoproteomic Analysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent of N-Dodecyl β-D-Maltoside

et al. 2015

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N-dodecyl β-D-maltoside (DDM), a mild detergent with the ability to maintain the enzyme activity and solubilize hydrophobic proteins without changing their structures, was applied for N-glycoproteomic analysis of minute protein sample from mouse brain tissue. After combining with the capillary-based glycoproteomic reactor, 281 N-glycosylation sites were successfully characterized from 50 μg of mouse brain tissue, which was 110% higher at least than those obtained by conventional strategies.

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“…carcinoma [62]. Similarly, it was used by Old and coworkers to identify differentially expressed proteins in K562 human erythroleukemia cells [63].…”

Section: Label-free Techniquesmentioning

confidence: 99%

High Throughput Sequencing and Proteomics to Identify Immunogenic Proteins of a New Pathogen: The Dirty Genome Approach

2014

Omics in Clinical Practice

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Accurate diagnosis and proper monitoring of cancer patients remain a key obstacle for successful cancer treatment and prevention. Therein comes the need for biomarker discovery, which is crucial to the current oncological and other clinical practices having the potential to impact the diagnosis and prognosis. In fact, most of the biomarkers have been discovered utilizing the proteomicsbased approaches. Although high-throughput mass spectrometry-based proteomic approaches like SILAC, 2D-DIGE, and iTRAQ are filling up the pitfalls of the conventional techniques, still serum proteomics importunately poses hurdle in overcoming a wide range of protein concentrations, and also the availability of patient tissue samples is a limitation for the biomarker discovery. Thus, researchers have looked for alternatives, and profiling of candidate biomarkers through tissue culture of tumor cell lines comes up as a promising option. It is a rich source of tumor cell-derived proteins, thereby, representing a wide array of potential biomarkers. Interestingly, most of the clinical biomarkers in use today (CA 125, CA 15.3, CA 19.9, and PSA) were discovered through tissue culture-based system and tissue extracts. This paper tries to emphasize the tissue culture-based discovery of candidate biomarkers through various mass spectrometry-based proteomic approaches.

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Development of quantitative plasma N-glycoproteomics using label-free 2-D LC-MALDI MS and its applicability for biomarker discovery in hepatocellular carcinoma

Cited by 40 publications

References 42 publications

Modification of Sialylation Mediates the Invasive Properties and Chemosensitivity of Human Hepatocellular Carcinoma

Modification of Sialylation Mediates the Invasive Properties and Chemosensitivity of Human Hepatocellular Carcinoma

High-Sensitivity N-Glycoproteomic Analysis of Mouse Brain Tissue by Protein Extraction with a Mild Detergent of N-Dodecyl β-D-Maltoside

High Throughput Sequencing and Proteomics to Identify Immunogenic Proteins of a New Pathogen: The Dirty Genome Approach

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