“…With the improvement on measurement throughout, speed and accuracy by Near-infrared reflectance spectroscopy (NIRS) analyzer, NIRS has been widely used in the non-destructive analysis of grain components, such as grain protein content, oil, fatty acids, dietary fiber, moisture, seed physical traits ( Cen and He, 2007 ). Compared with traditional laboratory methods, the NIRS methods have the great advantages of non-destructive, less time, low cost, and fast speed, and have been used for rapid phenotyping of quality traits in maize, soybean, rice, barley, oat and triticale ( Delwiche et al., 1996 ; Windham et al., 1997 ; Fox et al., 2011 ; Tarr et al., 2012 ; Han et al., 2017 ; Xu et al., 2019 ).…”