Development of potentiometric creatinine-sensitive biosensor based on ISFET and creatinine deiminase immobilised in PVA/SbQ photopolymeric membrane

Солдаткин, А. П.; Montoriol, Jean; Sant, W.; Martelet, C.; Jaffrézic‐Renault, Nicole

doi:10.1016/s0928-4931(02)00062-0

Cited by 42 publications

(25 citation statements)

References 19 publications

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“…Since this carbohydrate source decreases and there are only peptones in the culture medium, a second phase corresponding to an alkalization of the microenvironment appears. This result confirms those showing that the biological breakdown of peptones leads to NH 3 release and induces a pH increase [37,43,44]. This apparent substrate preference clearly led to differences in the quantity and quality of organic carbon substrates in the subsurface of the gel preparation.…”

Section: Bioprocess Monitoring With An Electrochemical Sensor Systemsupporting

confidence: 87%

Immobilization of E. coli bacteria in three-dimensional matrices for ISFET biosensor design

Bettaieb¹,

Ponsonnet²,

Lejeune³

et al. 2007

Bioelectrochemistry

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Section: Bioprocess Monitoring With An Electrochemical Sensor Systemsupporting

confidence: 87%

Immobilization of E. coli bacteria in three-dimensional matrices for ISFET biosensor design

Bettaieb¹,

Ponsonnet²,

Lejeune³

et al. 2007

Bioelectrochemistry

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“…The first example of a potentiometric biosensor for the detection of creatinine was proposed by Meyerhoff and Rechnitz, who used creatinine deiminase (CDI) coupled with an ammonia gas-sensing electrode (Meyerhoff and Rechnitz 1976). Latter, CDI immobilised in a bovine serum albumin (BSA) (Soldatkin et al 2002a) and PVA/SbQ membrane (Soldatkin et al 2002b) was used in combination with ion-sensitive field-effect transistors (ISFETs) for creatinine detection and hemodialysis control (Zinchenko et al 2012). However, these biosensors suffer from the inherent limitations of potentiometry, i.e.…”

Section: Introductionmentioning

confidence: 99%

Creatinine and urea biosensors based on a novel ammonium ion-selective copper-polyaniline nano-composite

Zhybak

Beni

Vagin

et al. 2016

Biosensors and Bioelectronics

101

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The use of a novel ammonium ion-specific copper-polyaniline nano-composite as transducer for hydrolase-based biosensors is proposed. In this work, a combination of creatinine deaminase and urease has been chosen as a model system to demonstrate the construction of urea and creatinine biosensors to illustrate the principle. Immobilisation of enzymes was shown to be a crucial step in the development of the biosensors; the use of glycerol and lactitol as stabilisers resulted in a significant improvement, especially in the case of the creatinine, of the operational stability of the biosensors (from few hours to at least 3 days). The developed biosensors exhibited high selectivity towards creatinine and urea. The sensitivity was found to be 85 ± 3.4 mAM(-1)cm(-2) for the creatinine biosensor and 112 ± 3.36 mAM(-1)cm(-2) for the urea biosensor, with apparent Michaelis-Menten constants (KM,app), obtained from the creatinine and urea calibration curves, of 0.163 mM for creatinine deaminase and 0.139 mM for urease, respectively. The biosensors responded linearly over the concentration range 1-125 µM, with a limit of detection of 0.5 µM and a response time of 15s. The performance of the biosensors in a real sample matrix, serum, was evaluated and a good correlation with standard spectrophotometric clinical laboratory techniques was found.

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“…A creatinine-sensitive biosensor has also been suggested using ion-sensitive field-effect transistor (ISFET) as a transducer and immobilized creatinine deiminase as a bioselective catalyst [9,10]. The amperometric biosensors are mainly based on trienzyme system that sequentially converts creatinine to creatine and creatine to sacrosine and finally sacrosine to glycine [11 -14].…”

Section: Introductionmentioning

confidence: 99%

“…The intrinsic characteristics of the detectors under hydrodynamic mode of operation in a low dispersion manifold are determined and compared with data obtained under static mode of operation. With 10 À2 mol L À1 phosphate buffer of pH 4.5 as a carrier solution, the tubular and coated wire CTP detectors exhibit rapid response of 58.9 and 50.7 mV decade À1 over the concentration range 1 Â 10 À2 -1 Â 10 À5 mol L À1 and detection limits of 0.39 mg mL À1 and 0.85 mg mL…”

mentioning

confidence: 99%

Novel Biomedical Sensors for Flow Injection Potentiometric Determination of Creatinine in Human Serum

2005

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Coated-wire (CW) and tubular (Tu) type membrane sensors for creatinine are developed. These consist of creatinine tungstophosphate(CTP), creatinine molybdophosphate (CMP) and creatinine picrolonate (CPC) ion-pair complexes as electroactive materials dispersed in plasticized poly(vinyl chloride) matrix membranes. Electrochemical evaluation of these sensors under static (batch) mode of operation reveals near-Nernstian response with slopes of 62.9, 58.1, and 55.2 mV decade À1 over the concentration range 1 Â 10 À2 -5.0 Â 10 À6, 1 Â 10 À2 -7.5 Â 10 À5, and 1 Â 10. The lower detection limits are 0.39, 3.49, and 2.20 mg mL À1 creatinine with CTP, CMP and CPC membrane based sensors plasticized with o-NPOE, respectively. Tubular and coated wire CTP membrane sensors are incorporated in flow-through cells and used as detectors for flow injection analysis (FIA) of creatinine. The intrinsic characteristics of the detectors under hydrodynamic mode of operation in a low dispersion manifold are determined and compared with data obtained under static mode of operation. With 10 À2 mol L À1 phosphate buffer of pH 4.5 as a carrier solution, the tubular and coated wire CTP detectors exhibit rapid response of 58.9 and 50.7 mV decade À1 over the concentration range 1 Â 10 À2 -1 Â 10 À5 mol L À1 and detection limits of 0.39 mg mL À1 and 0.85 mg mL À1, respectively. Validation of the assay methods with the proposed sensors by measuring the lower detection limit, range, accuracy, precision, repeatability and between-day-variability reveals good performance characteristics confirming applicability for continuous determination of creatinine. The sensors are used for determining creatinine in human blood serum at an input rate of 40 samples per hour. No interferences are caused by creatine, most common anions, cations and organic species normally present in biological fluids. The results favorably compare with data obtained using the standard spectrophotometric method.

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Development of potentiometric creatinine-sensitive biosensor based on ISFET and creatinine deiminase immobilised in PVA/SbQ photopolymeric membrane

Cited by 42 publications

References 19 publications

Immobilization of E. coli bacteria in three-dimensional matrices for ISFET biosensor design

Immobilization of E. coli bacteria in three-dimensional matrices for ISFET biosensor design

Creatinine and urea biosensors based on a novel ammonium ion-selective copper-polyaniline nano-composite

Novel Biomedical Sensors for Flow Injection Potentiometric Determination of Creatinine in Human Serum

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