Development of porous PLGA/PEI1.8k biodegradable microspheres for the delivery of mesenchymal stem cells (MSCs)

Lee, Young Sook; Lim, Kyutae; Oh, Jung Eun; Yoon, A-Rum; Joo, Wan Seok; Kim, Hyun Soo; Yun, Chae‐Ok; Kim, Sung Wan

doi:10.1016/j.jconrel.2015.01.004

Cited by 45 publications

(24 citation statements)

References 27 publications

(45 reference statements)

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“…Porous scaffolds, hydrogels, and cell carriers have been used to provide cells with a 3D microenvironment to support stem cell culture and differentiation [20-22, 29, 30]. Alternatively, a more physiologically relevant 3D microenvironment can be emulated through cell self-assembly, which has been applied to form multicellular aggregates in various cells types [31-33].…”

Section: Introductionmentioning

confidence: 99%

Mineral particles modulate osteo-chondrogenic differentiation of embryonic stem cell aggregates

et al. 2016

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Pluripotent stem cell aggregates offer an attractive approach to emulate embryonic morphogenesis and skeletal development. Calcium phosphate (CaP) based biomaterials have been shown to promote bone healing due to their osteoconductive and potential osteoinductive properties. In this study, we hypothesized that incorporation of CaP-coated hydroxyapatite mineral particles (MPs) within murine embryonic stem cell (ESC) aggregates could promote osteo-chondrogenic differentiation. Our results demonstrated that MP alone dose-dependently promoted the gene expression of chondrogenic and early osteogenic markers. In combination with soluble osteoinductive cues, MPs enhanced the hypertrophic and osteogenic phenotype, and mineralization of ESC aggregates. Additionally, MPs dose-dependently reduced ESC pluripotency and thereby decreased the size of teratomas derived from MP-incorporated ESC aggregates in vivo. Our data suggested a novel yet simple means of using mineral particles to control stem cell fate and create an osteochondral niche for skeletal tissue engineering applications.

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Section: Introductionmentioning

confidence: 99%

Mineral particles modulate osteo-chondrogenic differentiation of embryonic stem cell aggregates

et al. 2016

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“…Conventionally, the preparation of porous MPs has been achieved using template-assisted processing, porogen leaching methods, emulsion polymerization, self-assembly diffusion, reactive sintering methods and controlled solvent evaporation routes [7][8][9]. The electrospraying (ES) technique is a less common method for preparing porous MP structures, with the established modulation mechanism based on the solvent (polymer vehicle) evaporation rate.…”

Section: Introductionmentioning

confidence: 99%

Tuning Microparticle Porosity during Single Needle Electrospraying Synthesis via a Non-Solvent-Based Physicochemical Approach

et al. 2015

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Porous materials, especially microparticles (MP), are utilized in almost every field of engineering and science, ranging from healthcare materials (drug delivery to tissue engineering) to environmental engineering (biosensing to catalysis). Here, we utilize the single needle electrospraying technique (as opposed to complex systems currently in development) to prepare a variety of poly(ε-caprolactone) (PCL) MPs with diverse surface morphologies (variation in pore size from 220 nm to 1.35 µm) and architectural features (e.g., ellipsoidal, surface lamellar, Janus lotus seedpods and spherical). This is achieved by using an unconventional approach (exploiting physicochemical properties of a series of non-solvents as the collection media) via a single step. Sub-micron pores presented on MPs were visualized by electron microscopy (demonstrating a mean MP size range of 7-20 µm). The present approach enables modulation in morphology and size requirements for specific applications (e.g., pulmonary delivery, biological scaffolds, multi-stage drug delivery and biomaterial topography enhancement). Differences in static water contact angles were observed between smooth and porous MP-coated surfaces. This reflects the hydrophilic/hydrophobic properties of these materials.

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“…The major aim of tissue engineering is to restore, maintain or improve damaged tissues or whole organs, combining scaffolds, cells and biologically active molecules. Each of these elements is crucial for successful tissue regeneration . Cells provide the machinery for new tissue growth and differentiation and have to be selected accordingly.…”

Section: Introductionmentioning

confidence: 99%

“…Each of these elements is crucial for successful tissue regeneration. 1,2 Cells provide the machinery for new tissue growth and differentiation and have to be selected accordingly. Growth factors and other molecules modulate the cellular activity, providing stimuli for cells to differentiate while the scaffold provides the three-dimensional template structure supporting and facilitating these processes that are critical for tissue regeneration.…”

Section: Introductionmentioning

confidence: 99%

Dexamethasone-loaded biopolymeric nanoparticles promote gingival fibroblasts differentiation

Chronopoulou

Amalfitano

Palocci

et al. 2015

Biotechnol Progress

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Polymer-based nanoparticles (NPs) can be efficiently used for the delivery of bioactive molecules for both in vitro and in vivo applications affording high drug loading and controlled release profiles. Within this framework polylactic-co-glycolic acid (PLGA) NPs with a diameter of 290 ± 41 nm have been fabricated and loaded with dexamethasone (DXM) using a patented procedure. The aim of the project was to setup a controlled delivery system to promote the in vitro differentiation of Human Gingival Fibroblasts (HGFs). First the uptake of fluorescent PLGA NPs by HGFs cells was investigated; then experiments were also addressed to analyze the specific cell response to DXM, in order to evaluate its functional efficiency in comparison with its conventional addition to the culture medium. The results showed that cells treated with DXM-loaded NPs acquired the osteoblast phenotype faster in comparison to those treated with the free drug. The slow and sustained release of DXM from PLGA NPs produced a constant and uniform concentration of drug inside cells with long-term and enhanced biochemical effects.

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Development of porous PLGA/PEI1.8k biodegradable microspheres for the delivery of mesenchymal stem cells (MSCs)

Cited by 45 publications

References 27 publications

Mineral particles modulate osteo-chondrogenic differentiation of embryonic stem cell aggregates

Mineral particles modulate osteo-chondrogenic differentiation of embryonic stem cell aggregates

Tuning Microparticle Porosity during Single Needle Electrospraying Synthesis via a Non-Solvent-Based Physicochemical Approach

Dexamethasone-loaded biopolymeric nanoparticles promote gingival fibroblasts differentiation

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