Development of Populus alba L. and Populus tremula L. Species Specific Molecular Markers Based on the 5S rDNA Non-transcribed Spacer Polymorphism

Abstract

The Populus L. genus unites tree species, which are botanically grouped into several sections. The species successfully hybridize both in the same section and between some sections. The poplar hybridization widely occurs in nature and in variety breeding. Therefore, the development of poplar species specific molecular markers is very actual. The effective markers for trees of the Aigeiros Daby section have been recently developed using the polymorphism of the 5S rDNA non-transcribed spacers (NTSs). In this article, the 5S rDNA NTS based markers were designed for several species of the Leuce Daby section. The alb9 marker amplifies one fragment with DNA matrix of P. alba and P. × canescens (natural hybrid P. alba × P. tremula). The alb2 marker works the same way except the case with P. bolleana. In this case, the amplification of three fragments was observed. The tremu1 marker amplification is detected with DNA matrix of P. tremula and P. × canescens. Thus, the developed markers may be applied as useful tool for the P. alba, P. tremula, P. × canescens and P. bolleana identification in such areas of plant science as botany, dendrology, genetics of populations, variety breeding etc.

Analysis of the Aspen Growth (Populus tremula L.) Obtained by in Vitro in the Republic of Tatarstan

“…However, in the republic, there are highly productive clones resistant to fungal diseases. Therefore, today the most important task of foresters is the creation and cultivation of healthy and highly productive aspen plantations [2,3]. How to ensure that business aspen can be harvested in sufficient volumes and at the same time receive income to budgets of different levels?…”