Development of Polymerase Chain Reaction and Multiplex Polymerase Chain Reaction for Simple Identification of Thermoanaerobic Spore-forming Bacteria

Abstract

Mediated Isothermal Amplification (LAMP) primers were designed to develop a specific detection method forThermoanaerobacter mathranii and Thermoanaerobacter thermocopriae, highly problematic microbes in the food industry due to their extremely high resistance to heat and various antibacterial agents. Our LAMP method using the novel primers was able to easily detect these microbes. Our present methods effectively improve upon the complicated procedures employed in the quality control of raw materials and products in the food industry.