Development of polylactic acid (PLA) bio-composite films reinforced with bacterial cellulose nanocrystals (BCNC) without any surface modification

Wardhono, Endarto Yudo; Kanani, Nufus; Alfirano,; Rahmayetty,

doi:10.1080/01932691.2019.1626739

Cited by 28 publications

(12 citation statements)

References 48 publications

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“…The same condition occurred in a study whose objective was to use NCC as a reinforcement for a lm containing polylactic acid, concluding there was an increase in crystallinity (26). George et al, 2011 reported the utilization of NCC associated with polymers and showed there was an increase in the parameters of glass transition, melting temperature, enthalpy and crystalline behaviour.…”

Section: In Vitro Toxicitymentioning

confidence: 65%

Alginate-amphothericin B Nanocomplexes Covered by Nanocrystals From Bacterial Cellulose: Physico-chemical Characterization and in Vitro Toxicity

Vs¹,

Silva-Carvalho²,

Martins³

et al. 2020

Preprint

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Background: Nanostructured systems free of surfactants have pharmacotechnical and biopharmaceutical advantages, in addition to being a green process. Amphotericin B (AmB) is a drug with anti-leishmanial and anti-fungal potential, but its low water solubility and permeability limit its therapeutic use. Therefore, it could profit from being incorporated into nanostructured systems. In the present study, a self-assembled nanocomplex of alginate (Alg), a polysaccharide extracted from natural sources that can be used in the pharmaceutical area, with AmB was produced in order to improve the limited therapeutic use of this drug (Alg-AmB). Further, as a reinforcing component, cellulose nanocrystals (NCC) were ionically adsorbed into the surface of the nanocomplex systems (Alg-AmB + NCC). Results: Despite some polydispersity (0.523 ± 0.073), this straightforward process allowed to obtain water soluble particles with a hydrodynamic size of 258.87 ± 10.41 nm and charge of -62.93 ± 2.02 mV. Furthermore, the ionic adsorption of the NCC into the Alg-AmB nanocomplex surface was confirmed by an increase in the particle size (466.3 ± 17.57 nm) and a small surface charge decrease (-55.75 ± 1.23 mV). The amorphous inclusion complex of AmB into the polysaccharide chain network in both formulations was confirmed by DSC and FTIR. AmB in the nanocomplexes was in supper-aggregated form and showed good biocompatibility, being significantly less cytotoxic in vitro against kidney cells and significantly less hemolytic comparatively to the free-drug. Conclusions: The results were indicated the Alg-AmB nanocomplex can be considered an economical, non-toxic alternative to improve the AmB therapeutic effect. Furthermore, NCC coating of the nanocomplexes brought additional protection to the system without compromised the advantages attributed to the developed formulation.

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Section: In Vitro Toxicitymentioning

confidence: 65%

Alginate-amphothericin B Nanocomplexes Covered by Nanocrystals From Bacterial Cellulose: Physico-chemical Characterization and in Vitro Toxicity

Vs¹,

Silva-Carvalho²,

Martins³

et al. 2020

Preprint

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show abstract

“…About alginate sample, similar results were found by Silva-Carvalho et al (2020), indicating the importance of using safe polysaccharides for the formation of the nanocomplex. Another examples can be observed in a study whose objective was to use NCC as a reinforcement for a lm containing polylactic acid, concluding there was an increase in crystallinity 28 . George et al, (2011 reported the utilization of NCC associated with polymers and showed there was an increase in the parameters of glass transition, melting temperature, enthalpy and crystalline behaviour.…”

Section: Discussionmentioning

confidence: 97%

Alginate-Amphothericin B Nanocomplexes Covered By Nanocrystals From Bacterial Cellulose: Physico-Chemical Characterization And In Vitro Toxicity

Soeiro

Silva-Carvalho

Martins

et al. 2021

Preprint

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Amphotericin B (AmB) is a drug with anti-leishmanial and anti-fungal ability, but it has low water solubility and permeability, limiting its therapeutic use. Therefore, the incorporation of AmB into nanostructured systems could be profit. Nanostructured systems without surfactants have pharmacotechnical advantages such as amplify the water solubility and decrease the toxicity. For this reason, the present study aimed to produce a nanocomplex of alginate (Alg) with AmB covered by NCC in order to decrease the toxicity of AmB. This straightforward process allowed to obtain water soluble particles Furthermore, the ionic adsorption of the NCC into the Alg-AmB nanocomplex surface was confirmed by an increase in the particle size and a small surface charge decrease. The amorphous inclusion of AmB complex into the polysaccharide chain network in both formulations. AmB in the nanocomplexes was in supper-aggregated form and showed good biocompatibility, being significantly less cytotoxic in vitro against kidney cells and significantly less hemolytic compared to the free-drug. The results indicated the Alg-AmB nanocomplex can be considered a non-toxic alternative to improve the AmB therapeutic effect. Furthermore, NCC coating of the nanocomplexes brought additional protection to the system without compromised the advantages attributed to the developed formulation.

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“…By observing the difference in energy flow between the sample and reference, the thermal properties of materials can be measured [18]. DSC is widely used for the decomposition behavior determination of the polymer [19][20][21][22]. In the emulsion system, DSC monitors the solidification process inside the droplets and calculates the temperature dependence of the nucleation rate [23].…”

Section: Dsc Thermogram Interpretationmentioning

confidence: 99%

Calorimetry Technique for Observing the Evolution of Dispersed Droplets of Concentrated Water-in-Oil (W/O) Emulsion during Preparation, Storage and Destabilization

et al. 2019

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In this work, the evolution of dispersed droplets in a water-in-oil (W/O) emulsion during formation, storage, and destabilization was observed using a calorimetry technique. The emulsion was prepared by dispersing drop by drop an aqueous phase into an oil continuous phase at room temperature using a rotor-stator homogenizer. The evolution of droplets during (1) preparation; (2) storage; and (3) destabilization was observed using differential scanning calorimetry (DSC). The samples were gently cooled-down below its solid-liquid equilibrium temperature then heated back above the melting point to determine its freezing temperature. The energy released during the process was recorded in order to get information about the water droplet dispersion state. The mean droplet size distribution of the sample emulsion was correlated to its freezing temperature and the morphology was followed by optical microscopy. The results indicated that the calorimetry technique is so far a very good technique of characterization concentrated W/O emulsions.

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Development of polylactic acid (PLA) bio-composite films reinforced with bacterial cellulose nanocrystals (BCNC) without any surface modification

Cited by 28 publications

References 48 publications

Alginate-amphothericin B Nanocomplexes Covered by Nanocrystals From Bacterial Cellulose: Physico-chemical Characterization and in Vitro Toxicity

Alginate-amphothericin B Nanocomplexes Covered by Nanocrystals From Bacterial Cellulose: Physico-chemical Characterization and in Vitro Toxicity

Alginate-Amphothericin B Nanocomplexes Covered By Nanocrystals From Bacterial Cellulose: Physico-Chemical Characterization And In Vitro Toxicity

Calorimetry Technique for Observing the Evolution of Dispersed Droplets of Concentrated Water-in-Oil (W/O) Emulsion during Preparation, Storage and Destabilization

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