Development of polyclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for sodium saccharin residue in food samples

Wang, Yu; Xu, Zhen-Lin; Xie, Yong; Tian, Yuanxin; Shen, Yu-Dong; Young, Glenn M.; Wang, Hong; Lei, Hongtao; Sun, Yuan

doi:10.1016/j.foodchem.2010.11.076

Cited by 17 publications

(12 citation statements)

References 29 publications

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“…The detection limits of ELISA-based methods were usually above 10 3 CFU/mL, and the process generally required a long time. For instance, the indirect enzyme-linked immunosorbent assay (ELISA) took 6 to 7 h in total with an LOD of 10 5 CFU/mL . The novel Staphylococcal protein A enzyme-linked immunosorbent assay (SPA-ELISA) needed more than 12 h to detect A.…”

Section: Discussionmentioning

confidence: 99%

Development of the Saltatory Rolling Circle Amplification Assay for Rapid and Visual Detection of Alicyclobacillus acidoterrestris in Apple Juice

Yuan

Zhang

et al. 2020

J. Agric. Food Chem.

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A novel nucleic acid isothermal amplification method based on saltatory rolling circle amplification (SRCA) for rapid and visual detection of Alicyclobacillus acidoterrestris in apple juice was established. Fourteen A. acidoterrestris strains and 44 non-A. acidoterrestris strains were used to confirm the specificity. The sensitivity of SRCA was 4.5 × 10 1 CFU/mL by observing the white precipitate with the naked eye, while it was 4.5 × 10 0 CFU/mL by fluorescence visualization. The detection limit of SRCA in artificially inoculated apple juice was 7.1 × 10 1 and 7.1 × 10 0 CFU/mL via visualization of the white precipitate and fluorescence, respectively. Compared with the traditional PCR method, SRCA exhibited at least a 100-fold higher sensitivity and 100-fold lower detection limit. Seventy samples were investigated for A. acidoterrestris contamination, and the results showed 100% sensitivity, 97.01% specificity, and 97.14% accuracy compared with those by the conventional microbiological cultivation method. Overall, this method is a potentially useful tool for visual and rapid detection of A. acidoterrestris.

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Section: Discussionmentioning

confidence: 99%

Development of the Saltatory Rolling Circle Amplification Assay for Rapid and Visual Detection of Alicyclobacillus acidoterrestris in Apple Juice

Yuan

Zhang

et al. 2020

J. Agric. Food Chem.

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“…The icELISA protocol was performed as described previously [ 28 , 29 , 30 ]. Briefly, 100 µL/well of coating antigen (1 µg/mL in CBS) was added to 96-well microtiter plates and stored at 37 °C overnight.…”

Section: Methodsmentioning

confidence: 99%

A Sensitive Monoclonal-Antibody-Based ELISA for Forchlorfenuron Residue Analysis in Food Samples

et al. 2022

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In this study, forchlorfenuron (CPPU) was coupled with succinic anhydride to yield a CPPU hapten (CPPU-COOH), and a high-affinity monoclonal antibody (mAb) that can specifically recognize CPPU was produced. Using this mAb as a recognition reagent, a sensitive indirect competitive enzyme-linked immunosorbent assay (icELISA) for CPPU was optimized, which exhibits an IC50 of 1.04 ng/mL, a limit of detection of 0.16 ng/mL, and a linear range of 0.31–3.43 ng/mL for CPPU. Cross-reactivity percentages with six analogues were all below 6%. The average recovery rates for cucumber and orange samples were from 85.23% to 119.14%. The analysis results of this icELISA showed good consistency with those from liquid chromatography mass spectrometry. These results suggest that the proposed icELISA provides a sensitive, specific, and reliable strategy for CPPU detection in food samples.

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“…In the competitive immunoassay, the smaller B/B 0 is, the higher the sensitivity obtained. However, the sensitivity of competitive immunoassays would also be reduced if B 0 is too large or too small (Lee et al, 2006;Wang et al, 2010Wang et al, , 2011. Based on an overall consideration of B 0 and B/B 0 , the selected concentrations of AMOZA-OVA coating antigen and dilution of PCEPAMOZscFv-AP fusion protein were 0.25 mgL −1 and 1:8, respectively.…”

Section: Dcelisa Based On the Pcepamozscfv-ap Fusion Proteinmentioning

confidence: 99%

Development of a single-chain variable fragment-alkaline phosphatase fusion protein-based direct competitive enzyme-linked immunosorbent assay for furaltadone metabolite in ctenopharyngodon idellus

Chen

Hong

et al. 2021

Food and Agricultural Immunology

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To determine furaltadone metabolites 5-methylmorpholino-3amino-2-oxazolidinone (AMOZ) residual levels in ctenopharyngodon idellus, direct competitive enzyme-linked immunosorbent assay (dcELISA) was developed based on a single-chain variable fragment (scFv)-alkaline phosphatase (AP) fusion protein. Firstly, V H and V L gene sequences were cloned from hybridoma cell lines secreting monoclonal antibody against AMOZ derivative, and then the productive V H and V L genes were assembled to a scFv gene and engineered to produce scFv-AP fusion protein. The fusion protein was further identified as a bifunctional reagent for immunoassay, then a sensitive one-step dcELISA against AMOZ derivative was developed with a halfmaximal inhibitory concentration (IC 50 ) and limit of detection (LOD) of 10.62 and 4.83 ng/mL, respectively. The recovery values of AMOZ from spiked fish samples determined by dcELISA were in 71.31%∼82.88%. The results suggested that the prepared scFv-AP fusion protein in this work can be used for rapid and convenient immunoassays to detect AMOZ residues in fish samples.

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Development of polyclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for sodium saccharin residue in food samples

Cited by 17 publications

References 29 publications

Development of the Saltatory Rolling Circle Amplification Assay for Rapid and Visual Detection of Alicyclobacillus acidoterrestris in Apple Juice

Development of the Saltatory Rolling Circle Amplification Assay for Rapid and Visual Detection of Alicyclobacillus acidoterrestris in Apple Juice

A Sensitive Monoclonal-Antibody-Based ELISA for Forchlorfenuron Residue Analysis in Food Samples

Development of a single-chain variable fragment-alkaline phosphatase fusion protein-based direct competitive enzyme-linked immunosorbent assay for furaltadone metabolite in ctenopharyngodon idellus

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