Development of Piezoelectric Immunosensor for the Detection of Probiotic Bacteria

Szalontai, Helga; Adányi, Nóra; Kiss, Attila

doi:10.1080/00032719.2012.673095

Cited by 8 publications

(7 citation statements)

References 27 publications

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“…These are probiotic bacteria, which may also be used in the production of fermented foods. While a certain quantity of probiotics is required for a positive outcome, the number of colony-forming units (CFU) of pathogens in milk should be minimal, particularly if the milk is to be consumed directly and not processed further since bacterial cells multiply excessively under the right conditions [ 104 , 105 , 106 , 107 ].…”

Section: Application Of Baw and Saw Sensors And Biosensors In Milk Me...mentioning

confidence: 99%

“…Direct detection of the probiotics B. bifidum and L. acidophilus with antibody-coated QCM sensors resulted in a detection limit of 10 3 CFU/mL in milk diluted by a factor of 100. The highest detectable cell concentration was 5 × 10 5 CFU/mL [ 106 ]. A concentration within this measuring range, namely, 10 4 CFU/mL B. bifidum in about 50× diluted milk, was tested using a sandwich assay with gold-labeled antibodies [ 114 ].…”

Section: Application Of Baw and Saw Sensors And Biosensors In Milk Me...mentioning

confidence: 99%

“…The dilution successfully reduced interferences from the milk proteins, but the LOD in undiluted milk was correspondingly higher. However, the lowest acceptable bacteria concentration for probiotic products could still be detected since they should at least contain a concentration of 10 6 CFU/mL of probiotic bacteria [ 106 ].…”

Section: Application Of Baw and Saw Sensors And Biosensors In Milk Me...mentioning

confidence: 99%

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Bulk and Surface Acoustic Wave Biosensors for Milk Analysis

Länge

2022

Biosensors

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Milk and dairy products are common foods and, therefore, are subject to regular controls. Such controls cover both the identification and quantification of specific components and the determination of physical parameters. Components include the usual milk ingredients, mainly carbohydrates, proteins, and fat, and any impurities that may be present. The latter range from small molecules, such as drug residues, to large molecules, e.g., protein-based toxins, to pathogenic microorganisms. Physical parameters of interest include viscosity as an indicator of milk gelation. Bulk and surface acoustic wave sensors, such as quartz crystal microbalance (QCM) and surface acoustic wave (SAW) devices, can principally be used for both types of analysis, with the actual application mainly depending on the device coating and the test format. This review summarizes the achievements of acoustic sensor devices used for milk analysis applications, including the determination of physical liquid parameters and the detection of low- and high-molecular-weight analytes and microorganisms. It is shown how the various requirements resulting from the respective analytes and the complex sample matrix are addressed, and to what extent the analytical demands, e.g., with regard to legal limits, are met.

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Section: Application Of Baw and Saw Sensors And Biosensors In Milk Me...mentioning

confidence: 99%

Section: Application Of Baw and Saw Sensors And Biosensors In Milk Me...mentioning

confidence: 99%

Section: Application Of Baw and Saw Sensors And Biosensors In Milk Me...mentioning

confidence: 99%

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Bulk and Surface Acoustic Wave Biosensors for Milk Analysis

Länge

2022

Biosensors

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“…Furthermore, it is necessary to label the reagent and devise an optical measurement system. In contrast, a QCM sensor can measure the mass per unit area without the need for labeled reagents (Szalontai, Nóra Adányi, and Kiss 2012). However, QCM sensors are generally used in a tightly controlled environment, making it difficult to monitor deposition.…”

Section: Introductionmentioning

confidence: 98%

Inexpensive and Reliable Monitoring of the Microdeposition of Biomolecules

Fuchiwaki¹,

Tanaka²,

Ooie³

2015

Analytical Letters

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Anti-C-reactive protein antibody is used to quantify C-reactive protein in diagnostic tests for assessing inflammation levels. In order to develop a practical chip for diagnostic tests, anti-C-reactive protein antibody was continuously ejected using a piezoelectric injector and microdeposition was monitored on a shot-by-shot basis using a 30 MHz quartz crystal microbalance. Background noise due to external sources such as air flow and temperature fluctuations were largely eliminated by covering the quartz crystal microbalance with a stainless-steel cover and maintaining a fixed distance between the optical microscope lamp and the piezoelectric injector. Each shot of a 1 mg ml À1 solution of anti-C-reactive protein antibody solution delivered approximately 30 pl to the quartz crystal microbalance. The frequency change per shot was 2.21 AE 0.97 Hz and the injected mass was estimated to be 44.2 pg using the Sauerbrey equation. This approach simplifies the experimental evaluation of inkjet bioprinting by allowing the sensitive determination of shot ejection mass and volume, and realizes label-free detection, continuous monitoring, and simple temperature control. Measurements can be performed at atmospheric pressure and at room temperature. Furthermore, the system uses only commercially available devices.

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“…A QCM sensor for the detection of Salmonella typhimurium was developed and used for either direct or sandwich detection with gold nanoparticles [ 11 ]. Probiotic bacteria were detected in real samples in the range 10 4 –10 5 CFU·mL −1 within 60 min [ 12 ]. Non-labelled detection of Francisella tularensis was demonstrated by Kleo using QCM with dissipation monitoring and a detection limit of 4 × 10 3 CFU·mL −1 in 20 min [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM

Farka

Kovar

Skládal

2014

Sensors

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Label-free immunosensors are well suited for detection of microorganisms because of their fast response and reasonable sensitivity comparable to infection doses of common pathogens. Active (lever oscillator and frequency counter) and passive (impedance analyzer) modes of quartz crystal microbalance (QCM) were used and compared for rapid detection of three strains of E. coli. Different approaches for antibody immobilization were compared, the immobilization of reduced antibody using Sulfo‐SMCC was most effective achieving the limit of detection (LOD) 8 × 104 CFU·mL−1 in 10 min. For the passive mode, software evaluating impedance characteristics in real-time was developed and used. Almost the same results were achieved using both active and passive modes confirming that the sensor properties are not limited by the frequency evaluation method but mainly by affinity of the antibody. Furthermore, reference measurements were done using surface plasmon resonance. Effect of condition of cells on signal was observed showing that cells ruptured by ultrasonication provided slightly higher signal changes than intact microbes.

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Development of Piezoelectric Immunosensor for the Detection of Probiotic Bacteria

Cited by 8 publications

References 27 publications

Bulk and Surface Acoustic Wave Biosensors for Milk Analysis

Bulk and Surface Acoustic Wave Biosensors for Milk Analysis

Inexpensive and Reliable Monitoring of the Microdeposition of Biomolecules

Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM

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