Development of pH-responsive polymer and citrate aqueous two-phase system for extractive bioconversion of cefprozil

Chen, Jialing; Ding, Zhaoyang; Hong-xia, Pan; Cao, Xuejun

doi:10.1016/j.talanta.2017.05.027

Cited by 25 publications

(5 citation statements)

References 29 publications

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“…UCON has been employed for extraction of PEGylated lysozyme [52], protein purification [53], and purification of antibodies such as IgG [54]. Dong et al used a mixture of two pHresponsive polymers including P ADB4.91 and P ADB4.06 for extraction of transglutaminase [55], while Chen et al used P ADB6.8 as a pH-responsive polymer for cefprozil extractive bioconversion [56]. More applications of smart polymers in extraction and sample preparation of enzymes, hormones, and macromolecules are expected in the future.…”

Section: Homogeneous Phase Formation Using Smart Polymersmentioning

confidence: 99%

“…Using traditional polymers in large-scale extraction is rare, because of the high cost. So, the ability of smart polymers to be recycled substantially reduces the extraction cost and makes them a suitable alternative to commonly known polymers [55,56,123]. Bai et al [123] relied on using a pH-responsive polymer for efficient extraction of N-glycoprotein/glycol peptide as indicated in Figure 3A.…”

Section: Ph-induced Phase Separationmentioning

confidence: 99%

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Homogeneous liquid–liquid extraction as an alternative sample preparation technique for biomedical analysis

Hammad

Abdallah

Bedair

et al. 2021

J of Separation Science

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Liquid-liquid extraction is a widely used technique of sample preparation in biomedical analysis. In spite of the high pre-concentration capacities of liquidliquid extraction, it suffers from a number of limitations including time and effort consumption, large organic solvent utilization, and poor performance in highly polar analytes. Homogeneous liquid-liquid extraction is an alternative sample preparation technique that overcomes some drawbacks of conventional liquid-liquid extraction, and allows employing greener organic solvents in sample treatment. In homogeneous liquid-liquid extraction, a homogeneous phase is formed between the aqueous sample and the water-miscible extractant, followed by chemically or physically induced phase separation. To form the homogeneous phase, aqueous samples are mixed with water-miscible organic solvents, waterimmiscible solvents/cosolvents, surfactants, or smart polymers. Then, phase separation is induced chemically (adding salt, sugar, or buffer) or physically (changing temperature or pH). This mode is rapid, sustainable, and cost-effective in comparison with other sample preparation techniques. Moreover, homogeneous liquid-liquid extraction is more suitable for the extraction of delicate macromolecules such as enzymes, hormones, and proteins and it is more compatible with liquid chromatography with tandem mass spectrometry, which is a vital technique in metabolomics and proteomics. In this review, the principle, types, applications, automation, and technical aspects of homogeneous liquid-liquid extraction are discussed.

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Section: Homogeneous Phase Formation Using Smart Polymersmentioning

confidence: 99%

Section: Ph-induced Phase Separationmentioning

confidence: 99%

Homogeneous liquid–liquid extraction as an alternative sample preparation technique for biomedical analysis

Hammad

Abdallah

Bedair

et al. 2021

J of Separation Science

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“…The relaxation time was higher than the T22 relaxation time of PADB4.78 alone due to the presence of PN in the bottom phase. Such difference among the water-binding capability of polymers led to the occurrence of repulsion, reflecting the feasibility in forming aqueous two-phase system [6,32].…”

Section: Lf-nmr T2 Relaxation Time Measurementmentioning

confidence: 99%

Partition of Tea Saponin with a Novel Recyclable Thermo-pH Aqueous Two-Phase Systems

Wei

Chen

Yang

et al. 2021

Appl Biochem Biotechnol

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Aqueous two-phase systems (ATPS) have the advantages of environmentally friendly, high mass transfer efficiency, mild extraction conditions. However, it is difficult to recycle these components, which limits the large-scale application of ATPS in industrial production. In this study, a novel recyclable ATPS of PN/PADB4.78 was constructed with a thermo-responsive polymer PN and a pH-responsive polymer PADB4.78 for the partition study of tea saponin. The recovery of PN reached 98.71% by adjusting the temperature to above its lower critical solution temperature (LCST, 33.0 °C ) and the recovery of PADB4.78 was 97.32% by regulating the pH to its isoelectric point (pI, 4.78). Meanwhile, the phase formation mechanism of ATPS was studied by surface tension and low-field nuclear magnetic resonance (LF-NMR). The effects of polymer concentration, pH, temperature, types and concentrations of salt were investigated on tea saponin partition.In the 1.5% PN/3.5% PADB4.78 ATPS, the optimal partition coefficient (K) and extraction recovery (ERb) of crude tea saponin were 0.15 and 92.13% in the presence of 1.5 mM KCl at pH=7.6 and 25℃, respectively. The K and ERb of tea saponin from tea seeds were 0.12 and 94.50% with 7.5 mM LiBr at pH=8.0 and 25℃, respectively.

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“…ABS are endowed with a water-rich environment and an operating condition-dependent behavior, being deemed as mild extraction/separation processes. It has been shown that ABS can be used in several integrated biotech-based processes, such as in extractive bioconversions, , enzymatic reactions, and biopharmaceutical manufacturing . Furthermore, following the addition of ionic liquids (ILs) as ABS components, significant improvements have been accomplished in the efficiency, yield, and selectivity of numerous extraction and separation processes .…”

Section: Introductionmentioning

confidence: 99%

Double-Stimuli-Responsive (Temperature and pH) Aqueous Biphasic Systems Comprising Ionic Liquids

Rufino

Bola

Capela

et al. 2023

ACS Sustainable Chem. Eng.

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Aqueous biphasic systems (ABS) can integrate multiple unit operations and operate under continuous mode, contributing to the development of sustainable separation processes. Encouraged by the designer solvent features of ionic liquids (ILs), we herein propose their use as components of double-stimuli-responsive (temperature- and pH-driven) ABS. Resorting to choline-alkanoate-based ILs as the pH-responsive components and poly(propylene glycol) (PPG 400) as the thermo-responsive component, the ABS ternary phase diagrams are determined at various temperature (25–45 °C) and pH (3–7) conditions. While the liquid–liquid phase diagram response to temperature obeys a lower critical solution temperature-like behavior, the response to pH correlates with the pK a of the IL anion parent acid. The simultaneous responsiveness to temperature and pH is then shown, whose results inspire the development of customizable separation techniques as proved with the simultaneous (one-step) separation of two dyes. By a proper customization of the IL chemical structure and stimuli applied, ABS may be designed to improve the performance and sustainability of separation processes.

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Development of pH-responsive polymer and citrate aqueous two-phase system for extractive bioconversion of cefprozil

Cited by 25 publications

References 29 publications

Homogeneous liquid–liquid extraction as an alternative sample preparation technique for biomedical analysis

Homogeneous liquid–liquid extraction as an alternative sample preparation technique for biomedical analysis

Partition of Tea Saponin with a Novel Recyclable Thermo-pH Aqueous Two-Phase Systems

Double-Stimuli-Responsive (Temperature and pH) Aqueous Biphasic Systems Comprising Ionic Liquids

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