Development of PCR assays to Tri7 and Tri13 trichothecene biosynthetic genes, and characterisation of chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis

Chandler, E.; Simpson, D. R.; Thomsett, M.; Nicholson, P.

doi:10.1016/s0885-5765(03)00092-4

Cited by 184 publications

(199 citation statements)

References 29 publications

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“…The two isolates failed to PCR amplify in the Tri7DoN assay, but did amplify the expected fragments in both the minusTri7 and Tri13DoN assays; this PCR profile is consistent with the deletion of the entire Tri7 gene. Such a deletion is present in all isolates of F. culmorum tested to date (Chandler et al 2003;Kammoun et al 2010), but it is rather rare in F. graminearum (Lee et al 2001;Chandler et al 2003). Our results are consistent with the chemotype screenings from Eastern Europe, where 15-ADON chemotype dominates (Prodi et al 2009;Talas et al 2011), whereas 3-ADON chemotype is more frequent in Northen Europe (Jestoi et al 2008).…”

Section: Resultssupporting

confidence: 89%

“…DNA was extracted from the mycelia of each isolate using a CTAB method and the chemotype of each isolate (DON vs NIV) was determined using PCR assays directed at Tri7 and Tri13 genes according to Chandler et al (2003). The published primers for the Tri3 gene were used for the differentiation of the DON chemotype into either 3 or 15-ADON (Jennings et al 2004) (Table 2).…”

Section: Fusarium Isolates and Their Identificationmentioning

confidence: 99%

“…Gen Tri13 is responsible for the conversion of DON to NIV, while gen Tri7 acetylates NIV to produce 4-acetyl-nivalenol (4-ANIV) (Lee et al 2001(Lee et al , 2002. All DON producing F. graminearum isolates appear to carry mutations in both these two genes, whereas the NIV producing ones have functional Tri13 and Tri7 genes (Lee et al 2001;Chandler et al 2003).…”

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confidence: 99%

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Mycotoxin production, chemotypes and diversity of Czech Fusarium graminearum isolates on wheat

Sumíková¹,

Gabrielová²,

Kučera³

et al. 2013

Czech J. Food Sci.

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Sumíková T., Gabrielová L., Kučera L., Žabka M., Chrpová J. (2013): Mycotoxin production, chemotypes and diversity of Czech Fusarium graminearum isolates on wheat. Czech J. Food Sci., 31: 407-412.Fusarium head blight (FHB) is a serious cereal disease in the Czech Republic. The most important pathogen associated with FBH is Fusarium graminearum, which can produce trichothecenes, mainly deoxynivalenol (DON) and its derivates. A set of 103 F. graminearum isolates were isolated from naturally infected wheat ears collected from 20 localities (25 ears from one locality) within the Czech Republic, in the year 2004. The ears were evaluated for DON content by ELISA. Maximum detected value was 30.7 mg/kg. A group of PCR assays targeting the segments of the Tri7, Tri13 and Tri3 genes were used to determine the chemotypes of F. graminearum isolates. All the isolates belonged to DON producing chemotype. Further discrimination revealed that almost all (99.03%) isolates belonged to 15-ADON chemotype, and only one (0.97%) isolate belonged to 3-ADON chemotype. The genetic variability of the isolates was assessed from their AFLP fingerprints. The populations were highly heterogeneous both within and between locations, and no clear evidence for the association between AFLP profile and geographic origin was found out.

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Section: Resultssupporting

confidence: 89%

Section: Fusarium Isolates and Their Identificationmentioning

confidence: 99%

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Mycotoxin production, chemotypes and diversity of Czech Fusarium graminearum isolates on wheat

Sumíková¹,

Gabrielová²,

Kučera³

et al. 2013

Czech J. Food Sci.

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“…Based on this knowledge three genes were chosen in all studies to identify the chemotypes: tri3, tri5, tri7 and tri13 or regions between these genes. The developed assays were applied to Fusarium monitoring programs in numerous countries of Europe and Nortehrn America (Chandler et al, 2003;Jennings et al 2004;Lia et al, 2005;Quarta et al, 2006;Stepien et al, 2008;).…”

Section: Applications Of the Fusarium Pcr In Agriculture And Plant Pamentioning

confidence: 99%

Novel Methods for the Quantification of Pathogenic Fungi in Crop Plants: Quantitative PCR and ELISA Accurately Determine Fusarium Biomass

Brunner¹,

Farnleitner²,

Mach³

2012

Plant Pathology

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“…QTL associated with FHB resistance, Type I and Type II and more recently with DON detoxification (Type III sensu Miller and Arniston, 1986) were identified on almost all of the wheat chromosomes (Buerstmayr et al, 2009;Liu et al, 2009;Zhang et al, 2010). PCR markers for trichotecene genes (TRI) expression of Fusarium graminearum in order to predict occurrence of new chemotypes of F. graminearum and new techniques aimed to identify resistance to FHB in wheat were developed (Chandler et al, 2003;Gosman et al, 2010). That's why basic information about pathogenic ability in order to maximize the breeding efforts toward the management of FHB risks is needed.…”

mentioning

confidence: 99%

Phenotypic and marker assisted evaluation for pathogenicity and aggressiveness of Romanian Fusarium isolates vs. wheat

Ittu¹,

Cană²,

Ciucă³

et al. 2011

Plant Breeding and Seed Science

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Pathogenicity and aggressiveness vs. wheat of 30 new Fusarium accessions, primarily F. graminearum, obtained from random naturally infected grain samples of bread wheat, durum wheat and triticale collected across Romania, phenotypically and by molecular tools has been investigated. A large variation of this trait, expressed as reduction of coleoptiles length (% of control), in seedling stage, on average over three varieties, ranging from 2.1 to 30.9 % was registered. Point field inoculations at anthesis of 90 Fusarium isolate x wheat varieties combinations, revealed also the variability of several components of aggressiveness: severity (14.4-64.8%), AUDPC (104.9-527.1), and FDK 8.1-43.7%, respectively.Molecular techniques allowed identification of Fusarium species and the analysis of polymorphism within fungal isolates. Moreover, the presence of TRI 5 gene involved in DON biosynthesis was detected in the majority of isolates.Similarity between records obtained in seedling and adult stage for the most aggressive of Fusarium isolates, suggests that phenotypic selection in conjunction with molecular tools could be a reliable method to select the appropriate pathogen strains for breeding of resistance.

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Development of PCR assays to Tri7 and Tri13 trichothecene biosynthetic genes, and characterisation of chemotypes of Fusarium graminearum, Fusarium culmorum and Fusarium cerealis

Cited by 184 publications

References 29 publications

Mycotoxin production, chemotypes and diversity of Czech Fusarium graminearum isolates on wheat

Mycotoxin production, chemotypes and diversity of Czech Fusarium graminearum isolates on wheat

Novel Methods for the Quantification of Pathogenic Fungi in Crop Plants: Quantitative PCR and ELISA Accurately Determine Fusarium Biomass

Phenotypic and marker assisted evaluation for pathogenicity and aggressiveness of Romanian Fusarium isolates vs. wheat

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