Development of operating conditions for protein purification using expanded bed techniques: The effect of the degree of bed expansion on adsorption performance

Chang, Yu-Kaung; Chase, Howard A.

doi:10.1002/(sici)1097-0290(19960305)49:5<512::aid-bit4>3.0.co;2-m

Cited by 82 publications

(19 citation statements)

References 42 publications

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“…This result shows that the best alternative was to re-estimate the model parameters employing experimental data collected in an expanded bed. Chang and Chase (1996) had previously reported that the correlations in the literature did not correlate well with experimental data in expanded beds.…”

Section: Determination Of Adsorption Isotherms and Kineticsmentioning

confidence: 89%

“…Between the FB concentrations 50 and 100% (v/v), the latter showed the best results, mainly for the process efficiency cal 10% φ . φ , since for valuable products (as in the case of antibiotics) it is unlikely that the adsorbent batch will be loaded up to its total capacity, because the loss in antibiotic would increase the purification costs (Chang and Chase, 1996). Considering this fact, a FB concentration of 20% (v/v) represents the most promising option for vancomycin purification, since the process efficiencies were as high as 96% cal 90% ( ) φ and 31% cal 10% ( ) φ .…”

Section: Effect Of Changing the Batch Parametersmentioning

confidence: 99%

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Comparison of adsorption equilibrium and kinetic models for a case study of pharmaceutical active ingredient adsorption from fermentation broths: parameter determination, simulation, sensitivity analysis and optimization

Likozar

Senica²,

Pavko³

2012

Braz. J. Chem. Eng.

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-Mathematical models for a batch process were developed to predict concentration distributions for an active ingredient (vancomycin) adsorption on a representative hydrophobic-molecule adsorbent, using differently diluted crude fermentation broth with cells as the feedstock. The kinetic parameters were estimated using the maximization of the coefficient of determination by a heuristic algorithm. The parameters were estimated for each fermentation broth concentration using four concentration distributions at initial vancomycin concentrations of 4.96, 1.17, 2.78, and 5.54 g l −1 . In sequence, the models and their parameters were validated for fermentation broth concentrations of 0, 20, 50, and 100% (v/v) by calculating the coefficient of determination for each concentration distribution at the corresponding initial concentration. The applicability of the validated models for process optimization was investigated by using the models as process simulators to optimize the two process efficiencies.

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Section: Determination Of Adsorption Isotherms and Kineticsmentioning

confidence: 89%

Section: Effect Of Changing the Batch Parametersmentioning

confidence: 99%

Comparison of adsorption equilibrium and kinetic models for a case study of pharmaceutical active ingredient adsorption from fermentation broths: parameter determination, simulation, sensitivity analysis and optimization

Likozar

Senica²,

Pavko³

2012

Braz. J. Chem. Eng.

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“…However, at a settled bed height of 5.8 cm the reduced bed height appears to result in fluidizing instability; these trends are similar to those published previously for protein EBA. 18,19 The terminal velocities determined theoretically using the Stokes equation (Eqn (1)) are also provided 7 and Tong and Sun 12 considered that this was probably due to the irregular shape of the solid matrix or large size dispersion of the adsorbents.…”

Section: Bed Expansion Characteristicsmentioning

confidence: 99%

“…It is of importance to elucidate the expanded bed hydrodynamics for a better EBA process design. 5,6,18 Thus, the axial dispersion behavior in the expanded bed of the cation resin was investigated to confirm whether it is suitable for EBA operation.…”

Section: Axial Liquid-phase Dispersion Behavior In the Expanded Bedmentioning

confidence: 99%

Hydrodynamics in an expanded bed of large size ion‐exchange resin, and natural product adsorption

Chen

Wang

et al. 2007

J of Chemical Tech & Biotech

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Successful applications of expanded bed adsorption (EBA) technology have been widely reported in the literature for protein purification. Little has been reported on the recovery of natural products and active components of Chinese herbal preparations using EBA technology. In this study, the hydrodynamic behavior in an expanded bed of cation resin, 001 × 7 Styrene-DVB, was investigated. Ephedrine hydrochloride (EH) was used as a model natural product to test the dynamic binding capacity (DBC) in the expanded bed. EBA of EH directly from a feedstock containing powdered herbs has also been investigated. These particles are different from commercially available expanded bed adsorbents by virtue of their large size (205 to 1030 µm). When the adsorbent bed is expanded to approximately 1.3 to 1.5 times its settled bed height, the axial liquid-phase dispersion coefficient was found to be of the order 10 −5 m 2 s −1 , which falls into the range 1.0 × 10 −6 to 1.0 × 10 −5 m 2 s −1 observed previously in protein purification. Because of the favorable column efficiency (low axial dispersion coefficient), the recovery yield and purification factor values of EH directly from a feedstock reached 86.5% and 18, respectively. The results suggest that EBA technology holds promise for the recovery of natural products and active components of Chinese herbal preparations.

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“…A feedstock of lysozyme in buffer was chosen as this system demonstrates sharp, symmetrical breakthrough curves when applied to expanded beds of STREAMLINE SP [14]. By withdrawing samples from three different ports on the column, it was possible to obtain breakthrough curves at three positions up the length of the bed.…”

Section: Single Component Breakthrough Curvesmentioning

confidence: 99%

On-line monitoring of breakthrough curves within an expanded bed adsorber

Chase

Nash

Bruce

1999

Bioprocess Engineering

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By abstracting samples of the liquid phase from various positions along the height of an expanded bed, it has been possible to monitor the breakthrough pro®les of adsorbing components during the application of feedstock. Similarly, the concentration pro®les of the subsequent washing and elution procedures were also followed. The procedure involves the abstraction of liquid samples from the voids of the expanded bed using a specially modi®ed column and assaying the levels of proteins in the withdrawn stream by on-line rapid chromatographic monitoring. Studies of the residence time distribution showed that the modi®cations to the expanded bed did not cause additional mixing and dispersion. Breakthrough pro®les have been measured in a simple single component system and in a complex feedstock in which the adsorption of lysozyme from skimmed cows' milk was monitored. The system shows promise for the on-line control and monitoring of expanded bed adsorption separations, together with providing additional insight into the hydrodynamic and adsorption/desorption processes that occur during bioseparations using expanded bed adsorption. IntroductionOn-line monitoring and control of adsorption separations can have dramatic effects on process optimisation and ef®ciency [1±6]. Parameters critical to the ef®cient operation of the different stages of such a process include: Adsorption/loading stage. Ideally the duration of this phase should be such that the required component just starts to break through the column at a low level. Operation for longer periods results in wasteful loss of that component in the bed ef¯uent as it is no longer captured ef®ciently by the bed, whereas operation for shorter periods results in under-utilisation of the adsorption capacity of the adsorbent and hence the productivity of the bed is not as high as possible.Washing stage. During the washing phase, it is helpful to monitor the removal of certain key components from the bed. This ensures that the wash is continued for a suf®-cient period to ensure adequate removal of contaminants whilst minimising the consumption of wash buffer and possible loss of the required component as a result of the reversible nature of the adsorption process. Elution stage. During the elution phase, it is important to be able to detect the start and end of the peak containing the component of interest. This enables the required product to be collected in the minimum volume and at the highest yield. On-line knowledge of the levels of other eluting components allows fractions of the highest purity to be collected.Traditionally, the required information has been acquired by assaying the levels of key components in the ef¯uent stream from the bed. However, particularly for the monitoring of the loading stage of the procedure, this is inef®cient as the information obtained re¯ects the situation solely at the bed exit, rather than the more general situation within the bed. If control and termination of loading is based on detection of the level of breakthrough of the required ...

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Development of operating conditions for protein purification using expanded bed techniques: The effect of the degree of bed expansion on adsorption performance

Cited by 82 publications

References 42 publications

Comparison of adsorption equilibrium and kinetic models for a case study of pharmaceutical active ingredient adsorption from fermentation broths: parameter determination, simulation, sensitivity analysis and optimization

Comparison of adsorption equilibrium and kinetic models for a case study of pharmaceutical active ingredient adsorption from fermentation broths: parameter determination, simulation, sensitivity analysis and optimization

Hydrodynamics in an expanded bed of large size ion‐exchange resin, and natural product adsorption

On-line monitoring of breakthrough curves within an expanded bed adsorber

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