Development of one-step sample preparation methods for fatty acid profiling of milk fat

Liu, Zhiqian; Wang, Jianghui; Li, Cheng; Rochfort, Simone

doi:10.1016/j.foodchem.2020.126281

Cited by 11 publications

(10 citation statements)

References 40 publications

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“…However, in the case of Analytical Eco-Scale and GAPI, e.g., in the category of the amount of reagents used, the same score is obtained in the range of 10–100 mL. The E and F methods proposed by Liu et al [ 6 ] are eco-friendly, mainly due to the use of a small amount of sample (200 µL) and small amounts of toxic reagents. Additionally, and of great importance, they are the least labor-consuming and time-consuming.…”

Section: Resultsmentioning

confidence: 87%

“…In the literature [ 4 , 6 , 18 , 19 , 20 ] and international standards [ 21 , 22 , 23 ], there are many methods for the determination of FAs in a milk sample. Typically, FAMEs are quantified by GC following the multi-step sample preparation procedure.…”

Section: Resultsmentioning

confidence: 99%

“…In order to obtain fat, extraction with organic solvents (methods A, G and G’), centrifugation (C, D), and centrifugation combined with evaporation (B) were used. Additionally, we used the method of directly methylation in milk (E and F), which was presented by Liu et al [ 6 ]. The steps of sample preparation procedures used in our work was presented in Figure S1 ( Supplementary Material ).…”

Section: Resultsmentioning

confidence: 99%

“…The FA composition of milk fat is influenced by various factors such as animal genotypes, diet, lactation stage and the physiological state of the cows. As a result, analysis of the composition of FA is of great importance in lipid-related research and for the dairy industry [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

“…As mentioned above, the alkaline-catalyzed methylation is faster than acid-catalyzed methylation. Therefore, the alkaline-catalyzed methylation is often recommended for milk FA profiling [ 6 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of the Effectiveness and Environmental Impact of Selected Methods for the Determination of Fatty Acids in Milk Samples

Narloch

Wejnerowska

2022

Molecules

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Determination of the fatty acid profile in milk samples is one of the most important in food analysis. There are many methodologies for FA determination. The conventional procedure for determining the FA composition of milk is isolation of fat or indirect methylation, trans-methylation, extraction of fatty acids, and analysis by gas chromatography. In this study, eight methods based on alkaline methylation were compared for the analysis of fatty acids in cow’s milk. The response factors (RF) for GC analysis using FID were calculated. For most acids, RFs were close to 1, with the exception of short-chain fatty acids (C4:0–C8:0). To facilitate the selection of the method for the determination of fatty acids in milk samples, the methods were assessed using the environmental assessment tools of the analytical procedure: the Analytical Eco-Scale, Green Analytical Procedure Index (GAPI), and Analytical Greenness for Sample Preparation (AGREEprep). The method based on direct milk methylation received the highest scores. Omitting the lipid separation step has an impact on reducing the quantity of used toxic chemicals and reagents, and produces a smaller amount of waste, a much higher throughput, and a reduced cost analysis.

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Section: Resultsmentioning

confidence: 87%