Development of Omics Tools for the Assessments of the Environmental Fate and Impact of Biocontrol Agents

Ghosson, Hikmat; Salvia, Marie‐Virginie; Bertrand, Cédric

doi:10.1002/9781394188277.ch3

Cited by 3 publications

(8 citation statements)

References 70 publications

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“…It must be noted that the present extraction protocol is a part of a larger study that exclusively focuses on sample preparation and extraction protocols development. Five different extractions including the protocol described above were exhaustively assessed after application on two different types of soil and three different contamination conditions in order to set criteria for optimal extraction of herbicides residues and soil metabolites 13,15,16 …”

Section: Methodsmentioning

confidence: 99%

“…It is noteworthy that this work is part of a large ongoing project aiming to develop a novel analytical and chemometric approach dedicated to assess the environmental fate and impact of complex pesticides using MS‐based untargeted metabolomics 11–14 . Thus, the characterization of FI profiles done in this paper has a significant value for the future kinetics experiments where these compounds will be tracked through time in environmental matrices.…”

Section: Introductionmentioning

confidence: 99%

“…According to EU regulations, a maximum of toxicity data must be collected on FIs before authorizing a new pesticide on the market 2 . Ecotoxicity tests on terrestrial species (including soil microorganisms) are also required, either for AS (a priori risk assessment) or for FIs (a posteriori risk assessment) 3,4 . Nonetheless, most of analytical researches dedicated to pesticide analysis focuses on studying AS.…”

Section: Introductionmentioning

confidence: 99%

“…2 Ecotoxicity tests on terrestrial species (including soil microorganisms) are also required, either for AS (a priori risk assessment) or for FIs (a posteriori risk assessment). 3,4 Nonetheless, most of analytical researches dedicated to pesticide analysis focuses on studying AS. Less attention is given for FIs in the literature.…”

mentioning

confidence: 99%

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Characteristic response of formulation ingredients revealed by ultra high performance liquid chromatography‐electrospray ionization‐high resolution mass spectrometry‐based untargeted screening of pesticides in soil

et al. 2023

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Commercial solutions of pesticides consist of two main components: The active substance and the formulation ingredients. These ingredients, mainly composed of polymeric surfactants, are considered inert vis‐à‐vis the targeted organisms and nature. Nonetheless, a relatively low attention is given to their analysis and fate tracking in the environment. In this context, the current paper, embedded in a large study of fate and impact of formulated pesticides in soil, focuses on the analysis of these formulation ingredients. It mainly highlights and discusses the characteristic response of these ingredients in liquid chromatography–mass spectrometry‐based untargeted screening of two commercial herbicides applied on soil. This characteristic response is based on different spectral and chromatographic aspects, as their amplified adducts and double‐charged ions formation, or their “wavy” chromatographic profiles and the inversion of their elution order following the polymerization degree. These patterns are briefly discussed in order to explain them, and then thanks to their understanding, 12 different series (165 compounds) of formulation ingredients were outlined and discriminated from active substance and soil metabolites. After, high‐resolution and tandem mass spectrometry data were investigated for rapid interseries and intraseries identification‐by‐chain. In addition, recommendations for methods development and hints on postanalytical data processing for identity determination of these ingredients are given in order to help in enhancing future studies. Limitations of the applied approach are also outlined, and some innovate suggestions are provided based on the described findings.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

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Characteristic response of formulation ingredients revealed by ultra high performance liquid chromatography‐electrospray ionization‐high resolution mass spectrometry‐based untargeted screening of pesticides in soil

et al. 2023

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“…To proceed toward this development, five extractions (including three referenced , and two in-house developed) must be compared for two different types of agricultural soils in three different environmental conditions: soils contaminated with a commercial solution of synthetic polar herbicide, soils contaminated with a commercial solution of natural nonpolar herbicide, and uncontaminated control soils. The aim of this trifactorial experimental design (including 150 soil samples in total) is to allow the assessment of the five extraction protocols following four criteria: (1) The width of metabolome coverage in terms of molecular diversity and polarity, (2) the compromise between the width of metabolome coverage and the quantitative efficiency, (3) the reproducibility, and finally (4) the ability to discriminate between contaminated and uncontaminated soil samples (which is the key objective of the EMF). Nevertheless, the complexity of such an experimental design and the large size of the expected data set make the investigation of these criteria challenging.…”

Section: Introductionmentioning

confidence: 99%

LC–HRMS-Driven Computational Toolbox to Assess Extraction Protocols Dedicated to Untargeted Analysis: How to Ease Analyzing Pesticide-Contaminated Soils?

Ghosson,

Raviglione,

Bertrand

et al. 2024

Anal. Chem.

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Metabolomics is a powerful approach that allows for high throughput analysis and the acquisition of large biochemical data. Nonetheless, it still faces several challenging requirements, such as the development of optimal extraction and analytical methods able to respond to its high qualitative and quantitative requisites. Hence, the objective of the present article is to suggest a LC−HRMS-based untargeted profiling approach aiming to provide performant tools that help assess the performance and the quality of extraction methods. It is applied in a herbicide-contaminated soil metabolomics context. The trifactorial experimental design consists of 150 samples issued from five different extraction protocols, two types of soils, and three contamination conditions (contaminated soils with two different formulated herbicides against uncontaminated soils). Four performance and quality criteria are investigated using adapted LC−HRMS-driven computational tools. First, 861 metabolic features are annotated, and then the width of metabolome coverage and quantitative performance of the five different extraction protocols are assessed in all samples using various optimized configurations of heatmaps as well as van Krevelen diagrams. Then, the reproducibility of LC−HRMS profiles issued from the five extractions is studied by two different approaches: Euclidean distances and relative standard deviations. The two methods are examined and compared. Their advantages and limitations are thus discussed. After, the capacity of the different extractions to discriminate between contaminated and uncontaminated soils will be evaluated using orthogonal projections to latent structures-discriminant analysis. Different data scaling parameters are tested, and the results are explored and discussed. All of the suggested computational and visualization tools are performed using public-access platforms or open-source software. They can be readapted by metabolomics developers and users according to their study contexts and fields of application.

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Natural products for biocontrol: review of their fate in the environment and impacts on biodiversity

Amichot,

Bertrand,

Chauvel

et al. 2024

Environ Sci Pollut Res

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Development of Omics Tools for the Assessments of the Environmental Fate and Impact of Biocontrol Agents

Cited by 3 publications

References 70 publications

Characteristic response of formulation ingredients revealed by ultra high performance liquid chromatography‐electrospray ionization‐high resolution mass spectrometry‐based untargeted screening of pesticides in soil

Characteristic response of formulation ingredients revealed by ultra high performance liquid chromatography‐electrospray ionization‐high resolution mass spectrometry‐based untargeted screening of pesticides in soil

LC–HRMS-Driven Computational Toolbox to Assess Extraction Protocols Dedicated to Untargeted Analysis: How to Ease Analyzing Pesticide-Contaminated Soils?

Natural products for biocontrol: review of their fate in the environment and impacts on biodiversity

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