Development of olfactory marker protein and N-CAM expression in chemosensory systems of the opossum,Monodelphis domestica

Shapiro, Lena Shnayder; Roland, Risa Maxine; Halpern, Mimi

doi:10.1002/(sici)1097-4687(199711)234:2<109::aid-jmor1>3.0.co;2-7

Cited by 21 publications

(18 citation statements)

References 51 publications

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“…In order to gain a better understanding of the features and possible species differences in the anatomy and function in the two olfactory systems, immunocytochemical analysis was done of the neuronal expression patterns of three calcium-binding proteins, CB, PV, and CR, in the MOB and AOB in a marsupial species, the gray short-tailed opossum, Monodelphis domestica. This species has welldeveloped main olfactory and vomeronasal systems and has served as a subject in anatomical, behavioral, and developmental studies of the two systems (Fadem, 1987(Fadem, , 1989Stonerook and Harder, 1992;Brunjes et al, 1992;Poran et al, 1993;Philpot et al, 1994;Shapiro et al, 1995Shapiro et al, , 1996Shapiro et al, , 1997Shapiro et al, , 1998Halpern et al, 1995Halpern et al, , 1998aJackson and Harder, 1996;Cummings et al, 1997;Martinez-Marcos and Halpern, 1999a,b;Couper and Brunjes, 1999;Martinez-Marcos et al, 2000. We found that, while the overall patterns of immunostaining in the MOB were similar to those in other animals studied, a species difference in the expression of the three calcium-binding proteins was found both in the MOB and AOB, and the staining patterns were very different in the two bulbs.…”

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confidence: 60%

Calbindin D28k, parvalbumin, and calretinin immunoreactivity in the main and accessory olfactory bulbs of the gray short‐tailed opossum, Monodelphis domestica

Jia

Halpern

2004

Journal of Morphology

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The vertebrate main and accessory olfactory bulbs (MOB and AOB) are the first synaptic sites in the olfactory pathways. The MOB is a cortical structure phylogenetically well conserved in its laminar structure and overall synaptic organization, while the AOB has significant species variation in size. In order to better understand signal processing in the two olfactory systems and the species differences, immunocytochemical staining and analysis were done of the neuronal expression patterns of the calcium-binding proteins calbindin D28k (CB), parvalbumin (PV), and calretinin (CR) in the MOB and AOB in a marsupial species, the gray short-tailed opossum, Monodelphis domestica. In the MOB, antibody to CB labeled periglomerular cells, superficial short axon cells / Van Gehuchten cells; antibody to PV labeled Van Gehuchten cells; and antibody to CR immunostained periglomerular cells, superficial short axon cells / Van Gehuchten cells, and granule cells. In the AOB, CB immunoreactivity was detected in periglomerular cells and a subpopulation of granule cells; antibody to PV labeled the superficial short axon cells / Van Gehuchten cells and granule cells; and antibody to CR labeled a small number of periglomerular cells, superficial short axon cells / Van Gehuchten cells, and granule cells. These results showed that the patterns of CB, PV, and CR expression differ in the opossum main and accessory olfactory bulbs and differ from that in other animal species. These varying patterns of neuronal immunostaining may be related to the different functions of the main and accessory olfactory bulbs and to the differing signal processing features.

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confidence: 60%

Calbindin D28k, parvalbumin, and calretinin immunoreactivity in the main and accessory olfactory bulbs of the gray short‐tailed opossum, Monodelphis domestica

Jia

Halpern

2004

Journal of Morphology

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“…Although the function of OMP is uncertain, it has been related to transduction (Buiakova et al, 1996) and neurogenesis (Carr et al, 1998), and it has been demonstrated that OMP is almost exclusively expressed in mature neurons (Monti Graziadei et al, 1977;Farbman and Margolis, 1980;Miragall and Monti Graziadei, 1982). Interestingly, in contrast to mice where OMP is expressed throughout the receptor cell layer (Monti Graziadei et al, 1980;Tarozzo et al, 1998), in adult opossums, OMP is strongly expressed only in the upper neuronal layer (Shapiro et al, 1997). Thus, coexpression of BrdU, G proteins and OMP has been used to study the differential maturation of subclasses of vomeronasal neurons.…”

Section: Differential Migration and Maturation Of Subclasses Of Vomermentioning

confidence: 99%

Cell turnover in the vomeronasal epithelium: Evidence for differential migration and maturation of subclasses of vomeronasal neurons in the adult opossum

2000

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Previous investigations of cell turnover in the mammalian vomeronasal sensory epithelium (VN‐SE) raised two issues. First, if, in addition to the already demonstrated vertical migration, horizontal migration from the edges of the VN‐SE participates in neuronal replacement. Second, whether or not migration and maturation is differential in upper and lower populations of vomeronasal neurons, since these two cell populations are chemically, physiologically, functionally, and perhaps evolutionarily different. By injecting bromodeoxyuridine (BrdU) into adult opossum (Monodelphis domestica) and permitting different survival times, the pattern of distribution of BrdU‐labeled cells was analyzed. No evidence of horizontal migration in neuronal replacement was found. To investigate vertical migration and maturation of subclasses of vomeronasal neurons, double immunohistochemistry of BrdU and markers of the lower (Goα protein) and upper [Gi2α protein and olfactory marker protein (OMP)] cell populations were performed. Three days after administration of BrdU, some mature neurons were observed in both lower and upper layers of the VN‐SE, as demonstrated by coexpression of BrdU with Goα protein and OMP, respectively. The data on vertical distribution, however, indicate that most of the daughter cells enter the Goα‐protein–expressing zone of the VN‐SE by day 5, whereas most daughter cells do not reach the Gi2α‐protein–expressing zone until day 7, suggesting that these two populations mature at slightly different rates. These results are the first evidence of differential neurogenesis of subclasses of vomeronasal neurons. © 2000 John Wiley & Sons, Inc. J Neurobiol 43: 50–63, 2000

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“…In these glomeruli the primary olfactory afferent fibres make synaptic contacts with the dendrites of mitral and tufted cells. It has been reported that during development, a few olfactory fibres can be observed that overgrow the target glomeruli and enter deeper layers of the olfactory bulb [4,11,15–17]. In these studies, olfactory projection fibres, characterized by the presence of the olfactory marker protein (OMP), were seen as deep as the internal plexiform layer.…”

Section: Introductionmentioning

confidence: 99%

“…According to Gong and Shipley [4], the misrouted (MR) olfactory fibres may play a role in the induction of development of olfactory bulb structures. This seems in contradiction with the fact that, in opossum, these fibres lack the neural cell adhesion molecule (NCAM) [17] that plays a role in cell migration and the stabilization of cell contacts as well as the guidance of axons [10]. Other authors regard these fibres as axons that, by accident, simply overgrow their targets [16,17].…”

Section: Introductionmentioning

confidence: 99%

“…This seems in contradiction with the fact that, in opossum, these fibres lack the neural cell adhesion molecule (NCAM) [17] that plays a role in cell migration and the stabilization of cell contacts as well as the guidance of axons [10]. Other authors regard these fibres as axons that, by accident, simply overgrow their targets [16,17]. If this is the case, it is surprising that they do so only in a short period during development because the olfactory receptor cells and their axons are replaced throughout the lifetime.…”

Section: Introductionmentioning

confidence: 99%

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Misrouted olfactory fibres and ectopic olfactory glomeruli in normal humans and in Parkinson and Alzheimer patients

Hoogland

Berg

Huisman

2003

Neuropathology Appl Neurobio

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The presence and distribution of misrouted (MR) olfactory projection fibres were studied in the olfactory bulbs of control human brains and in the brains of people who had suffered from Parkinson's and Alzheimer's disease. It appeared that MR fibres, that is, fibres that terminate deep into the glomerular layer, are a common phenomenon in the bulbs of aged people. In all but one of the bulbs studied, MR fibres are present. The amount of MR fibres is not related to age. With a few exceptions, the MR fibres are restricted to the external plexiform layer (EPL). Only in Parkinson bulbs the MR fibres occasionally form glomerulus-like structures. These pseudo glomeruli are located in the EPL. It is concluded that MR olfactory projection fibres are a normal phenomenon in the human olfactory bulb. In nonhuman mammalians, MR fibres have only been observed in foetal and neonatal olfactory bulbs. Possibly, the age-related loss of mitral cells, which are the natural synaptic targets for the olfactory projection fibres, may play a role in the aberrant behaviour of the MR olfactory receptor cell axons. The ectopic glomerulus-like structures in Parkinson bulbs share some characteristics with normal rat glomeruli that are not observed in normal human olfactory glomeruli. This may refer to possible changes in the genetic content of olfactory structures in Parkinson patients.

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Development of olfactory marker protein and N-CAM expression in chemosensory systems of the opossum,Monodelphis domestica

Cited by 21 publications

References 51 publications

Calbindin D28k, parvalbumin, and calretinin immunoreactivity in the main and accessory olfactory bulbs of the gray short‐tailed opossum, Monodelphis domestica

Calbindin D28k, parvalbumin, and calretinin immunoreactivity in the main and accessory olfactory bulbs of the gray short‐tailed opossum, Monodelphis domestica

Cell turnover in the vomeronasal epithelium: Evidence for differential migration and maturation of subclasses of vomeronasal neurons in the adult opossum

Misrouted olfactory fibres and ectopic olfactory glomeruli in normal humans and in Parkinson and Alzheimer patients

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