Development of Nutraceutical Product

Yusof, Yus Aniza; Etti, Christopher Joseph; Chin, Nyuk Ling

doi:10.18517/ijaseit.5.3.511

Cited by 7 publications

(4 citation statements)

References 16 publications

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“…In addition to the above finding, the current study suggests that the chitosan SETs supports the initial attachment, spreading and proliferation of HFSCs in vitro. This phenomenon is likely due to close binding between the positively charged chitosan and the negatively charged cells [21]. Other reports have demonstrated that both fibroblasts and keratinocytes attached to and proliferated well on a chitosan-based scaffold [33], [34].…”

Section: Attachment and Proliferation Of Hfscs On Chitosan Setsmentioning

confidence: 85%

“…Natural polymeric scaffolds, including collagen-, hydrogel-or chitosan-based scaffolds, have been the focus, as they have favourable biomechanical properties, significant nutraceutical properties [21], [22] and are both biodegradable and biocompatible [10]. In contrast, synthetic materials are found to generate acidic products that cause onsite inflammation, which is harmful to the surrounding tissue [23].…”

Section: K Microstructure Of Chitosan Setsmentioning

confidence: 99%

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Proliferation and Differentiation of Human Hair Follicle Stem Cells on Chitosan-Skin Engineered Template in Vitro

Hilmi

Norhayati

Halim

et al. 2017

International Journal on Advanced Science, Engineering and Information Technology

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Hair follicles repeatedly regress and reconstitute themselves, suggesting the presence of intrinsic tissue stem cells. Among the unique characteristics of adult stem cells isolated from hair follicles is their ability to differentiate into keratinocytes. Study on chitosan skin-engineered templates (SETs) as scaffolds for the proliferation of human fibroblasts have shown the promise of SETs in facilitating skin cell growth in three-dimensional culture. High proliferation in three-dimensional culture using human cells allows the researcher to extensively evaluate the cultivation of desirable cell types on chitosan SETs. Therefore, this study aimed to evaluate the in vitro attachment, proliferation and differentiation of hair follicle stem cells (HFSCs) on a chitosan SETs. HFSCs were isolated from human scalp tissues using collagenase type I prior to propagation in supplemented CnT-07 media. The phenotype of the HFSCs was verified using the markers keratin-15 (K15) and CD200, as detected by immunocytochemical staining. The attachment and proliferation of the HFSCs on the chitosan SETs were evaluated using scanning electron microscopy (SEM), an alamar blue assay and a live/dead assay. Subsequently, the HFSCs were differentiated using CnT-2D differentiation media. The cells' differentiation was verified using the markers involucrin and keratin-6 (K6), as detected by immunofluorescence staining. The HFSCs were successfully isolated, proliferated and differentiated according to staining positivity and microscopy imaging. HFSCs are able to proliferate and directly differentiate into keratinocytes on a chitosan SETs, which may facilitate their use in regenerative medicine.

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Section: Attachment and Proliferation Of Hfscs On Chitosan Setsmentioning

confidence: 85%

Section: K Microstructure Of Chitosan Setsmentioning

confidence: 99%

Proliferation and Differentiation of Human Hair Follicle Stem Cells on Chitosan-Skin Engineered Template in Vitro

Hilmi

Norhayati

Halim

et al. 2017

International Journal on Advanced Science, Engineering and Information Technology

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“…Good compressibility of a dosage form is needed to encourage drug development in industrial pharmacy. Optimization of particle size and density generally leads to a corresponding improvement in compressibility (Yusof et al, 2015) . In a study by Sun et al (2019) the formulation of ca eine (CAF) and methyl gallate in a cocrystal form provides increased compressibility to CAF tablets.…”

Section: Compressibility and Palatabilitymentioning

confidence: 99%

Crystal Engineering Approach in Physicochemical Properties Modifications of Phytochemical

Lutfiyah

Fitriani

Taher

et al. 2022

Sci. Technol. Indones

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Phytochemicals have been used to reduce the risk of diseases and maintain good health and well-being. However, most phytochemicals have a limitation in their physicochemical properties, which can be modified by reforming the shape of the crystals. Therefore, crystal engineering is a promising approach to optimize physicochemical characteristics of the active pharmaceutical ingredients (APIs) in a phytochemical without altering its pharmacological efficacy. Hence, this paper reviews current strategies for the use of crystal engineering to optimize physicochemical properties of phytochemicals, which is followed by the design of the synthesis and characterization of particular phytochemicals, including piperine (PIP), quercetin (QUE), curcumin (CUR), genistein (GEN), and myricetin (MYR). The literature indicates that crystal engineering of multicomponent crystals (MCCs) enhances phytochemical physicochemical properties, including solubility, dissolution rate, stability, and permeability. The MCCs provide a lower lattice energy and noncovalent bonding, which translate into lower melting points and weak intermolecular interactions that generate greater solubility, higher dissolution rate, and better stability of the APIs. Nevertheless, the absence of reported studies of phytochemical crystal engineering leads to a lack of variation in the selection of coformers, methods of preparation, and improvement of physicochemical properties. Therefore, more extensive evaluation of the design and physicochemical characteristics of phytochemicals using MCCs is necessary and manifests the opportunity to enhance the application of phytochemicals in the pharmaceutical industry.

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“…Nutraceuticals are food or parts of food that provide medical or health benefits, including the prevention and treatment of diseases. 16,17 A few researchers have investigated plants like the Centella asiatica, Ginkgo biloba, and Polygonum minus for their neuroprotective properties in older people, with neuropsychological tests as outcomes. 11,13,18 To determine the potential ability of nutraceuticals to prevent neurodegenerative diseases, it is necessary to implement a different approach for the assessment of cognitive functions, that is, by determining the level of activation of the brain, this this case, with a functional MRI (fMRI) .…”

mentioning

confidence: 99%

Neuroimaging Functional Magnetic Resonance Imaging Task‐Based Dorsolateral Prefrontal Cortex Activation Following 12 Weeks of Cosmos caudatus Supplementation Among Older Adults With Mild Cognitive Impairment

You

Shahar

Mohamad

et al. 2021

Magnetic Resonance Imaging

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Background: Cosmos caudatus (CC) is traditional Asian vegetable, commonly consumed among the Southeast Asian population. It has been reported to be high in flavonoids and might potentially improve brain activity among older adults with mild cognitive impairment (MCI). The effect of CC in brain activation improvement using neuroimaging is yet to be discovered. Purpose: To investigate the effects of CC supplement on brain activity using functional magnetic resonance imaging (fMRI) among older adults with MCI. Study type: Prospective, randomized, double-blind, placebo-controlled trial. Population/subjects: Twenty older adults with mild cognitive impairment (60-75 years old), 14 of them (70%) were female subjects. Field strength/sequence: A 3.0-T, T1-weighted anatomical images, T2*-weighted imaging data, A single shot, gradient echo-echo planar imaging (EPI) sequence. Assessment: All subjects were asked to consume two 500 mg capsules of either CC supplement or placebo (maltodextrin) daily for 12 weeks. Cognitive function was measured using validated neuropsychological tests (i.e. Mini-mental State Examination and Digit Span) and task-based fMRI (N-back and Stroop Color Word Test) at baseline and 12th week. Brodmann's area 9, 46 and anterior cingulate cortex were selected as the regions of interest to define dorsolateral prefrontal cortex (DLPFC) in fMRI analysis. Statistical tests: Normality test was performed with the Shapiro-Wilk test. Two-way repeated ANOVA determined the intervention effects of the CC supplementation on brain activity after adjustments for covariates. Significance level at P < 0.05 for independent-t test and Chi square test; adjusted P < 0.0042 for two-way repeated ANOVA after Bonferroni correction. Results: Findings showed significant improvements in digit span (partial η 2 = 0.559), increment in right DLPFC activation while performing 1-back task (partial η 2 = 0.586) and left DLPFC activation while performing Stroop Color Word Test (SCWT) (congruent) task (partial η 2 = 0.432) at 12th week of CC supplementation.

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Development of Nutraceutical Product

Cited by 7 publications

References 16 publications

Proliferation and Differentiation of Human Hair Follicle Stem Cells on Chitosan-Skin Engineered Template in Vitro

Proliferation and Differentiation of Human Hair Follicle Stem Cells on Chitosan-Skin Engineered Template in Vitro

Crystal Engineering Approach in Physicochemical Properties Modifications of Phytochemical

Neuroimaging Functional Magnetic Resonance Imaging Task‐Based Dorsolateral Prefrontal Cortex Activation Following 12 Weeks of Cosmos caudatus Supplementation Among Older Adults With Mild Cognitive Impairment

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