Development of novel polymorphic microsatellite markers for the silver fox (Vulpes vulpes)

Yan, Shouqing; Bai, Chen; Qi, S M; Li, Y M; Li, W J; Sun, Jun-hong

doi:10.4238/2015.june.1.6

Cited by 4 publications

(4 citation statements)

References 13 publications

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“…Tissue samples were stored at −20°C or in absolute ethanol. We genotyped the samples using 16 microsatellite loci: DGN3, DGN14, FH2541, REN161A12, REN162B09, REN69B24, V374, V402, V502, Vv‐C01.424, Vv‐C08.618, Vv‐CPH11, Vv‐CPH2, Vv‐INU055, VVM124, VVM189 (Breen et al, ; Mariat, Amigues, & Boscher, ; Moore, Brown, & Sacks, ; Wandeler & Funk, ; Yan et al, ). The data are publicly available (see Data Accessibility).…”

Section: Methodsmentioning

confidence: 99%

Beyond the landscape: Resistance modelling infers physical and behavioural gene flow barriers to a mobile carnivore across a metropolitan area

et al. 2020

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Urbanization affects key aspects of wildlife ecology. Dispersal in urban wildlife species may be impacted by geographical barriers but also by a species’ inherent behavioural variability. There are no functional connectivity analyses using continuous individual‐based sampling across an urban‐rural continuum that would allow a thorough assessment of the relative importance of physical and behavioural dispersal barriers. We used 16 microsatellite loci to genotype 374 red foxes (Vulpes vulpes) from the city of Berlin and surrounding rural regions in Brandenburg in order to study genetic structure and dispersal behaviour of a mobile carnivore across the urban‐rural landscape. We assessed functional connectivity by applying an individual‐based landscape genetic optimization procedure. Three commonly used genetic distance measures yielded different model selection results, with only the results of an eigenvector‐based multivariate analysis reasonably explaining genetic differentiation patterns. Genetic clustering methods and landscape resistance modelling supported the presence of an urban population with reduced dispersal across the city border. Artificial structures (railways, motorways) served as main dispersal corridors within the cityscape, yet urban foxes avoided densely built‐up areas. We show that despite their ubiquitous presence in urban areas, their mobility and behavioural plasticity, foxes were affected in their dispersal by anthropogenic presence. Distinguishing between man‐made structures and sites of human activity, rather than between natural and artificial structures, is thus essential for better understanding urban fox dispersal. This differentiation may also help to understand dispersal of other urban wildlife and to predict how behaviour can shape population genetic structure beyond physical barriers.

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Section: Methodsmentioning

confidence: 99%

Beyond the landscape: Resistance modelling infers physical and behavioural gene flow barriers to a mobile carnivore across a metropolitan area

et al. 2020

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“…Genotyping was carried out using a multiplex PCR of 20 autosomal microsatellite loci (RF 21,59,125,127,131,143,155,156,162,165,199,200 65 ; VVM 219, 85, 838, 529, 189, 844, 828 66 ), explicitly developed for the red fox.…”

Section: Genetic Analysismentioning

confidence: 99%

Nutritional ecology of a prototypical generalist predator, the red fox (Vulpes vulpes)

Balestrieri,

Gigliotti,

Caniglia

et al. 2024

Sci Rep

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Generalist species, which exploit a wide range of food resources, are expected to be able to combine available resources as to attain their specific macronutrient ratio (percentage of caloric intake of protein, lipids and carbohydrates). Among mammalian predators, the red fox Vulpesvulpes is a widespread, opportunistic forager: its diet has been largely studied, outlining wide variation according to geographic and climatic factors. We aimed to check if, throughout the species’ European range, diets vary widely in macronutrient composition or foxes can combine complementary foods to gain the same nutrient intake. First, we assessed fox’s intake target in the framework of nutritional geometry. Secondly, we aimed to highlight the effects of unbalanced diets on fox density, which was assumed as a proxy for Darwinian fitness, as assessed in five areas of the western Italian Alps. Unexpectedly, the target macronutrient ratio of the fox (52.4% protein-, 38.7% lipid- and 8.9% carbohydrate energy) was consistent with that of hypercarnivores, such as wolves and felids, except for carbohydrate intakes in urban and rural habitats. The inverse relation between density and the deviation of observed macronutrient ratios from the intake target suggests that fox capability of surviving in a wide range of habitats may not be exempt from fitness costs and that nutrient availability should be regarded among the biotic factors affecting animal abundance and distribution.

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“…Genotyping was carried out using a multiplex PCR of 20 autosomal microsatellite loci (RF 21,59,125,127,131,143,155,156,162,165,199,200 41 ; VVM 219, 85, 838, 529, 189, 844, 828 42 ), explicitly developed for the red fox.…”

Section: Genetic Analysismentioning

confidence: 99%

Nutritional ecology of a prototypical generalist predator, the red fox (Vulpes vulpes)

Balestrieri,

Gigliotti,

Caniglia

et al. 2024

Preprint

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Generalist species, which exploit a wide range of food resources, are expected to be able to combine available resources as to attain their specific macronutrient balance (intake target). Among mammalian predators, the red fox Vulpes vulpes is a widespread, opportunistic forager: its diet has been largely studied, outlining wide variation according to geographic and climatic factors. We aimed to check if, throughout the species’ European range, diets vary widely in macronutrient composition or foxes can combine complementary foods to gain the same nutrient intake. First, we assessed fox’s intake target in the framework of nutritional geometry. Secondly, we tried to highlight the effects of unbalanced diets on fox density, which was assumed as a proxy for Darwinian fitness, as assessed in five areas of the western Italian Alps. Unexpectedly, the target macronutrient ratio of the fox (52.4% protein-, 38.7% lipid- and 8.9% carbohydrate energy) was consistent with that of hypercarnivores, such as wolves and felids, except for carbohydrate intakes in urban and rural habitats. The inverse relation between density and the deviation of observed macronutrient ratios from the intake target suggests that fox capability of surviving in a wide range of habitats may not be exempt from fitness costs and that nutrient availability should be regarded among the biotic factors affecting animal abundance and distribution.

show abstract

Development of novel polymorphic microsatellite markers for the silver fox (Vulpes vulpes)

Cited by 4 publications

References 13 publications

Beyond the landscape: Resistance modelling infers physical and behavioural gene flow barriers to a mobile carnivore across a metropolitan area

Beyond the landscape: Resistance modelling infers physical and behavioural gene flow barriers to a mobile carnivore across a metropolitan area

Nutritional ecology of a prototypical generalist predator, the red fox (Vulpes vulpes)

Nutritional ecology of a prototypical generalist predator, the red fox (Vulpes vulpes)

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