Development of novel microsatellite markers for population differentiation and detection of natural selection in wild populations of Butter catfish, Ompok bimaculatus (Bloch, 1794)
Abstract:Background: Butter catfish (Ompok bimaculatus) is a preferred species in South East Asia, with huge aquaculture potential. However, there is limited information about genetic stock composition due to insufficient markers. The goal of this study was to develop de novo microsatellite markers.
Methods and Results: For sequencing, genomic SMRT bell libraries (1.5 Kbp size) were prepared for O. bimaculatus. A total of 114 SSR containing sequences were used for primer designing and tested. Polymorphic loci were vali… Show more
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