Development of novel methodology for the molecular differentiation of Cryptosporidium parvum gp60 subtypes via high resolution melting analysis

Leary, Jennifer K. O’; Blake, Liam; Corcoran, G.D.; Sleator, Roy D.; Lucey, Brigid

doi:10.1016/j.mex.2020.101157

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…Subtype assignment is based on the number of trinucleotide repeats and other repetitive sequences. Subtype families are named Ia, etc., for C. hominis and IIa, etc., for C. parvum [ 61 ].…”

Section: Methodsmentioning

confidence: 99%

First Molecular Characterization of Cryptosporidium spp. in Patients Living with HIV in Honduras

Betancourth

Archaga

Moncada³

et al. 2021

Pathogens

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Cryptosporidiosis is one of the most important causes of gastroenteritis in the world, especially in low- and middle-income countries. It is caused by the Apicomplexan parasite Cryptosporidium spp., and mainly affects children and immunocompromised people, in whom it can pose a serious threat to their health, or even be life threatening. In Honduras, there are no data on parasite species or on molecular diversity or Cryptosporidium subtypes. Therefore, a cross-sectional study was conducted between September 2019 and March 2020 for the molecular identification of Cryptosporidium spp. in 102 patients living with HIV who attended a national hospital in Tegucigalpa. Stool samples were analyzed by direct microscopy, acid-fast stained smears, and a rapid lateral flow immunochromatographic test. All samples that tested positive were molecularly analyzed to identify the species and subtype of the parasite using three different markers: gp60, cowp, and 18Sr. PCR products were also sequenced. Four out of 102 samples (3.92%) were positive for Cryptosporidiumparvum, and all were assigned to subtype IIa. These findings suggest a possible zoonotic transmission in this population.

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“…Subtype assignment is based on the number of trinucleotide repeats and other repetitive sequences. Subtype families are named Ia, etc., for C. hominis and IIa, etc., for C. parvum [ 61 ].…”

Section: Methodsmentioning

confidence: 99%

First Molecular Characterization of Cryptosporidium spp. in Patients Living with HIV in Honduras

Betancourth

Archaga

Moncada³

et al. 2021

Pathogens

View full text Add to dashboard Cite

show abstract

“…A number of subtyping instruments based on the glycoprotein 60-kDa ( gp60 ) gene were developed to identify the human source of Cryptosporidium infection precisely [ 9 – 11 ]. The proposed and widely adopted gp60 nomenclature system has greatly facilitated the analysis of global epidemiology, as the variability in the sequence of this marker has been instrumental in inferring transmission routes [ 12 ]. However, there is still uncertainty regarding the epidemiology of Cryptosporidium and its spread in humans, particularly in underdeveloped regions of the world, requiring further research.…”

Section: Introductionmentioning

confidence: 99%

Genetic characterizations of Cryptosporidium spp. from children with or without diarrhea in Wenzhou, China: high probability of zoonotic transmission

Zhao,

Ren,

Jiang

et al. 2024

BMC Microbiol

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Background Cryptosporidium is a highly pathogenic parasite responsible for diarrhea in children worldwide. Here, the epidemiological status and genetic characteristics of Cryptosporidium in children with or without diarrhea were investigated with tracking of potential sources in Wenzhou City, China. Methods A total of 1032 children were recruited, 684 of whom had diarrhea and 348 without, from Yuying Children’s Hospital in Wenzhou, China. Samples of stool were collected from each participant, followed by extraction of DNA, genotyping, and molecular identification of Cryptosporidium species and subtypes. Results Twenty-two of the 1032 (2.1%) children were infected with Cryptosporidium spp. with 2.5% (17/684) and 1.4% (5/348) in diarrhoeic and asymptomatic children, respectively. Four Cryptosporidium species were identified, including C. parvum (68.2%; 15/22), C. felis (13.6%; 3/22), C. viatorum (9.1%; 2/22), and C. baileyi (9.1%; 2/22). Two C. parvum subtypes named IIdA19G1 (n = 14) and IInA10 (n = 1), and one each of C. felis (XIXa) and C. viatorum (XVaA3g) subtype was found as well. Conclusions This is the first research that identified Cryptosporidium in children of Wenzhou, China, using PCR. Identification of zoonotic C. parvum, C. felis, C. viatorum, and their subtypes indicate potential cross-species transmission of Cryptosporidium between children and animals. Additionally, the presence of C. baileyi in children suggests that this species has a wider host range than previously believed and that it possesses the capacity to infect humans.

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Development of novel methodology for the molecular differentiation of Cryptosporidium parvum gp60 subtypes via high resolution melting analysis

Cited by 2 publications

References 25 publications

First Molecular Characterization of Cryptosporidium spp. in Patients Living with HIV in Honduras

First Molecular Characterization of Cryptosporidium spp. in Patients Living with HIV in Honduras

Genetic characterizations of Cryptosporidium spp. from children with or without diarrhea in Wenzhou, China: high probability of zoonotic transmission

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