Development of novel functional ingredients: Need for testing systems and solutions with Caenorhabditis elegans

Calvo, Daniel; Martorell, Patricia; Genovés, Salvador; Gosálbez, Luis

doi:10.1016/j.tifs.2016.05.006

Cited by 26 publications

(19 citation statements)

References 21 publications

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“…This opens the possibility to produce tailored models for specific purposes, such as inducing a disease-related phenotype, reproducing molecular disease mechanisms or performing molecular mechanistic studies with dietary interventions. Indeed, C. elegans fills in the gap between in vitro and in vivo approaches, allowing a high-throughput reductionist approach, providing at the same time physiologically relevant data derived from a whole-animal [ 37 , 157 ].…”

Section: Final Remarksmentioning

confidence: 99%

“…A clear advantage of C. elegans over other animal models derives from its regulatory status. Despite it is not true, it is not legally defined as an animal in European, USA or Canadian conventions, which excludes it from ethical limitations for its experimental usage, contrary to vertebrates, like mice, rats or zebrafish, that are protected by stringent animal rights regulations [ 157 ]. A summary of the main characteristics of C. elegans compared with other ex vivo and in vivo models is collected in the Table 2 .…”

Section: Final Remarksmentioning

confidence: 99%

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Caenorhabditis elegans as a Model Organism to Evaluate the Antioxidant Effects of Phytochemicals

et al. 2020

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The nematode Caenorhabditis elegans was introduced as a model organism in biological research by Sydney Brenner in the 1970s. Since then, it has been increasingly used for investigating processes such as ageing, oxidative stress, neurodegeneration, or inflammation, for which there is a high degree of homology between C. elegans and human pathways, so that the worm offers promising possibilities to study mechanisms of action and effects of phytochemicals of foods and plants. In this paper, the genes and pathways regulating oxidative stress in C. elegans are discussed, as well as the methodological approaches used for their evaluation in the worm. In particular, the following aspects are reviewed: the use of stress assays, determination of chemical and biochemical markers (e.g., ROS, carbonylated proteins, lipid peroxides or altered DNA), influence on gene expression and the employment of mutant worm strains, either carrying loss-of-function mutations or fluorescent reporters, such as the GFP.

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Section: Final Remarksmentioning

confidence: 99%

Section: Final Remarksmentioning

confidence: 99%

Caenorhabditis elegans as a Model Organism to Evaluate the Antioxidant Effects of Phytochemicals

et al. 2020

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“…The nematode genome contains more than 18,000 genes where 60% to 80% are homologue to human ones. Besides the highly conserved metabolic pathways, its easy-to-maintain, short cycle of live, economical, and large collection of mutants are some of the main advantages of the use of C. elegans [8,9].…”

Section: Introductionmentioning

confidence: 99%

Edible Flowers of Tagetes erecta L. as Functional Ingredients: Phenolic Composition, Antioxidant and Protective Effects on Caenorhabditis elegans

et al. 2018

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Tagetes erecta L. has long been consumed for culinary and medicinal purposes in different countries. The aim of this study was to explore the potential benefits from two cultivars of T. erecta related to its polyphenolic profile as well as antioxidant and anti-aging properties. The phenolic composition was analyzed by LC-DAD-ESI/MSn. Folin-Ciocalteu, DPPH·, and FRAP assays were performed in order to evaluate reducing antiradical properties. The neuroprotective potential was evaluated using the enzymes acetylcholinesterase and monoamine oxidase. Caenorhabditis elegans was used as an in vivo model to assess extract toxicity, antioxidant activity, delayed aging, and reduced β-amyloid toxicity. Both extracts showed similar phenolic profiles and bioactivities. The main polyphenols found were laricitin and its glycosides. No acute toxicity was detected for extracts in the C. elegans model. T. erecta flower extracts showed promising antioxidant and neuroprotective properties in the different tested models. Hence, these results may add some information supporting the possibilities of using these plants as functional foods and/or as nutraceutical ingredients.

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“…C. elegans is a suitable in vivo model for research on the molecular biology and genetics of different diseases as well as for drug and bioactive compound screenings [ 43 ]. C. elegans models present alternative approaches to understanding neurodegenerative diseases for which there are currently few effective therapies [ 6 ].…”

Section: Discussionmentioning

confidence: 99%

Tryptophan-Containing Dual Neuroprotective Peptides: Prolyl Endopeptidase Inhibition and Caenorhabditis elegans Protection from β-Amyloid Peptide Toxicity

Manzanares

Martı́nez

Garrigues

et al. 2018

IJMS

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Neuroprotective peptides represent an attractive pharmacological strategy for the prevention or treatment of age-related diseases, for which there are currently few effective therapies. Lactoferrin (LF)-derived peptides (PKHs) and a set of six rationally-designed tryptophan (W)-containing heptapeptides (PACEIs) were characterized as prolyl endopeptidase (PEP) inhibitors, and their effect on β-amyloid peptide (Aβ) toxicity in a Caenorhabditis elegans model of Alzheimer’s disease (AD) was evaluated. Two LF-derived sequences, PKH8 and PKH11, sharing a W at the C-terminal end, and the six PACEI heptapeptides (PACEI48L to PACEI53L) exhibited significant in vitro PEP inhibition. The inhibitory peptides PKH11 and PACEI50L also alleviated Aβ-induced paralysis in the in vivo C. elegans model of AD. Partial or total loss of the inhibitory effect on PEP was achieved by the substitution of W residues in PKH11 and PACEI50L and correlated with the loss of protection against Aβ toxicity, pointing out the relevance of W on the neuroprotective activity. Further experiments suggest that C. elegans protection might not be mediated by an antioxidant mechanism but rather by inhibition of Aβ oligomerization and thus, amyloid deposition. In conclusion, novel natural and rationally-designed W-containing peptides are suitable starting leads to design effective neuroprotective agents.

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Development of novel functional ingredients: Need for testing systems and solutions with Caenorhabditis elegans

Cited by 26 publications

References 21 publications

Caenorhabditis elegans as a Model Organism to Evaluate the Antioxidant Effects of Phytochemicals

Caenorhabditis elegans as a Model Organism to Evaluate the Antioxidant Effects of Phytochemicals

Edible Flowers of Tagetes erecta L. as Functional Ingredients: Phenolic Composition, Antioxidant and Protective Effects on Caenorhabditis elegans

Tryptophan-Containing Dual Neuroprotective Peptides: Prolyl Endopeptidase Inhibition and Caenorhabditis elegans Protection from β-Amyloid Peptide Toxicity

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