Development of novel formulations containing Lysozyme and Lactoferrin and evaluation of antibacterial effects on Mutans Streptococci and Lactobacilli

Tonguc-Altin, K.; Sandallı, Nüket; Duman, Gülengül; Selvi-Kuvvetli, Senem; Topçuoğlu, Nursen; Külekçi, Güven

doi:10.1016/j.archoralbio.2015.02.004

Cited by 23 publications

(17 citation statements)

References 44 publications

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“…Other components (e.g., xylitol, sorbitol, system of enzymes, fluoride ions) have antimicrobial properties, prevent growing of harmful flora. Results of this study show that preparation B, based on xylitol, hydroxyethylcellulose and system of enzymes, exhibits the best antimicrobial activity, confirming previous reports [33,34], indicating that those enzymes were successfully used as main antimicrobial agents.…”

Section: Resultssupporting

confidence: 88%

Antimicrobial and Physicochemical Properties of Artificial Saliva Formulations Supplemented with Core-Shell Magnetic Nanoparticles

Niemirowicz-Laskowska

Mystkowska

Łysik

et al. 2020

IJMS

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Saliva plays a crucial role in oral cavity. In addition to its buffering and moisturizing properties, saliva fulfills many biofunctional requirements, including antibacterial activity that is essential to assure proper oral microbiota growth. Due to numerous extra- and intra-systemic factors, there are many disorders of its secretion, leading to oral dryness. Saliva substitutes used in such situations must meet many demands. This study was design to evaluate the effect of core-shell magnetic nanoparticles (MNPs) adding (gold-coated and aminosilane-coated nanoparticles NPs) on antimicrobial (microorganism adhesion, biofilm formation), rheological (viscosity, viscoelasticity) and physicochemical (pH, surface tension, conductivity) properties of three commercially available saliva formulations. Upon the addition of NPs (20 µg/mL), antibacterial activity of artificial saliva was found to increase against tested microorganisms by 20% to 50%. NPs, especially gold-coated ones, decrease the adhesion of Gram-positive and fungal cells by 65% and Gram-negative bacteria cells by 45%. Moreover, the addition of NPs strengthened the antimicrobial properties of tested artificial saliva, without influencing their rheological and physicochemical properties, which stay within the range characterizing the natural saliva collected from healthy subjects.

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Section: Resultssupporting

confidence: 88%

Antimicrobial and Physicochemical Properties of Artificial Saliva Formulations Supplemented with Core-Shell Magnetic Nanoparticles

Niemirowicz-Laskowska

Mystkowska

Łysik

et al. 2020

IJMS

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“…Addition of exogenous lactoferrin restored function in mice genetically deficient in lactoferrin 24 . Potentially, the use of saliva as a diagnostic/prognostic indicator, using multivariate analysis of both host and microbial factors, could allow exogenous host defence peptides or other antimicrobial proteins to be a targeted approach to groups most likely to benefit 25 .…”

Section: Discussionmentioning

confidence: 99%

Combined analysis of the salivary microbiome and host defence peptides predicts dental disease

Simón-Soro

Sherriff

Sadique

et al. 2018

Sci Rep

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Understanding the triad of host response, microbiome and disease status is potentially informative for disease prediction, prevention, early intervention and treatment. Using longitudinal assessment of saliva and disease status, we demonstrated that partial least squares modelling of microbial, immunological and clinical measures, grouped children according to future dental disease status. Saliva was collected and dental health assessed in 33 children aged 4 years, and again 1-year later. The composition of the salivary microbiome was assessed and host defence peptides in saliva were quantified. Principal component analysis of the salivary microbiome indicated that children clustered by age and not disease status. Similarly, changes in salivary host defence peptides occurred with age and not in response to, or preceding dental caries. Partial least squares modelling of microbial, immunological and clinical baseline measures clustered children according to future dental disease status. These data demonstrate that isolated evaluation of the salivary microbiome or host response failed to predict dental disease. In contrast, combined assessment of both host response together with the microbiome revealed clusters of health and disease. This type of approach is potentially relevant to myriad diseases that are modified by host–microbiome interactions.

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“…In research on new drugs aimed at inhibiting the growth of cariogenic colonies of bacteria living in the oral cavity, Tonguc-Altin et al proved that, thanks to the appropriate carriers and in combination with lactoferrin, lysozyme can be used to reduce the amount of S. mutans and L. acidophilus [ 99 ].…”

Section: Determination Of Enzymesmentioning

confidence: 99%

A Review of Selected Studies That Determine the Physical and Chemical Properties of Saliva in the Field of Dental Treatment

Kubala

Strzelecka

Grzegocka

et al. 2018

BioMed Research International

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Physiological whole saliva is a unique body fluid constantly washing the mucous membranes of the mouth, throat, and larynx. Saliva is a clear, slightly acidic mucinous-serous secretion, composed of various electrolytes, small organic substances, proteins, peptides, and polynucleotides. There are many ways to use saliva as a biological fluid (biofluid). The significant advantages of saliva as a unique diagnostic material are its availability and the noninvasive method of collection. The aim of this review is to emphasize the diagnostic value of saliva as a research material in the configuration of its structure and secretion disorders. The data were obtained using the MEDLINE (PubMed) search engine, as well as an additional manual search. The analysis covered 77 articles selected from a group of 1986 publications and initially qualified for devising. The results were evaluated and checked for the correctness of qualifying in accordance with inclusion and exclusion criteria. The diagnostic use of saliva has attracted the attention of many researchers due to its noninvasive nature and relative simplicity of collection. In addition, it should be noted that the determination of chemical and physical saliva parameters can be effectively performed in the patient's presence in the dental office.

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Development of novel formulations containing Lysozyme and Lactoferrin and evaluation of antibacterial effects on Mutans Streptococci and Lactobacilli

Cited by 23 publications

References 44 publications

Antimicrobial and Physicochemical Properties of Artificial Saliva Formulations Supplemented with Core-Shell Magnetic Nanoparticles

Antimicrobial and Physicochemical Properties of Artificial Saliva Formulations Supplemented with Core-Shell Magnetic Nanoparticles

Combined analysis of the salivary microbiome and host defence peptides predicts dental disease

A Review of Selected Studies That Determine the Physical and Chemical Properties of Saliva in the Field of Dental Treatment

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