Development of novel collagen scaffolds with different bioceramic particles for bone tissue engineering

Arahira, Takaaki; Todo, Mitsugu

doi:10.1016/j.coco.2019.08.012

Cited by 14 publications

(9 citation statements)

References 16 publications

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“…Young′s modulus of nHA composite scaffolds used for bone tissue engineering are 2.97 and 43.03 MPa, respectively [31]. Moreover, many investigations have shown that the compression stress of collagen-based scaffolds used as bone tissue materials is lower than 3.0 MPa [8,17,32]. In contrast, our nHD scaffolds showed significantly enhanced mechanical properties.…”

Section: Structure Of the Scaffolds Before And After Enzymatic Degradmentioning

confidence: 76%

Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Liu

Fan

2020

Polymers

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A novel, three-dimensional, porous, human-like collagen (HLC)/nano-hydroxyapatite (n-HA) scaffold cross-linked by 1,2,7,8-diepoxyoctane (DEO) was successfully fabricated, which showed excellent mechanical and superior biological properties for bone tissue regeneration in this study. The physicochemical characterizations of different n-HA/HLC/DEO (nHD) scaffolds were investigated by determining the morphology, compression stress, elastic modulus, Young’s modulus and enzymatic hydrolysis behavior in vitro. The results demonstrated that nHD-2 and nHD-3 scaffolds showed superior mechanical properties and resistance to enzymatic hydrolysis compared to nHD-1 scaffolds. The cell viability, live cell staining and cell adhesion analysis results demonstrated that nHD-2 scaffolds exhibited low cytotoxicity and excellent cytocompatibility compared with nHD-1 and nHD-3 scaffolds. Furthermore, subcutaneous injections of nHD-2 scaffolds in rabbits produced superior anti-biodegradation effects and histocompatibility compared with injections of nHD-1 and nHD-3 scaffolds after 1, 2 and 4 weeks. In addition, the repair of bone defects in rabbits demonstrated that nHD-2 scaffolds presented an improved ability for guided bone regeneration and reconstruction compared to commercially available bone scaffold composite hydroxyapatite/collagen (HC). Collectively, the results show that nHD-2 scaffolds show promise for application in bone tissue engineering due to their excellent mechanical properties, anti-biodegradation, anti-biodegradation, biocompatibility and bone repair effects.

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Section: Structure Of the Scaffolds Before And After Enzymatic Degradmentioning

confidence: 76%

Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Liu

Fan

2020

Polymers

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“…At sampling point, they were removed from the PBS solution and weighed for a weight of m2. Water absorption ratio = (m2 – m1)/m1 × 100% . All experiments were repeated for three times.…”

Section: Methodsmentioning

confidence: 99%

Effects of Fiber Cross-Angle Structures on the Mechanical Property of 3D Printed Scaffolds and Performance of Seeded MC3T3-E1 Cells

et al. 2021

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The three-dimensional (3D) printing technology combined with bone tissue engineering has become one of the major methods for mandibular reconstruction. However, the key factor retarding mandible reconstruction is the barrier of understanding and achieving the complex 3D gridwork formed by the trabeculae. This study innovatively constructed a low-temperature 3D printing silk fibroin/collagen/hydroxyapatite (SF/COL/HA) composite scaffold with a stable structure and remarkable biocompatibility. We designed three kinds of six-layer scaffolds with mixed fiber cross-angle structures (FCAS) of [0°/90°/0°/90°/0°/90°], [0°/45°/90°/135°/180°/225°] and [0°/30°/60°/90°/120°/150°]. Material properties of these scaffolds such as porosity, water absorption rate, X-ray diffraction, Fourier transform infrared spectroscopy, and compression performance were detected. Then, the MC3T3-E1 cells were seeded on these scaffolds and the adhesion, proliferation, and differentiation were investigated. To be more convincing, the same experiments were performed on another polycaprolactone/hydroxyapatite scaffold. The results suggested that the changes of FCAS affected the mechanical properties of 3D printed scaffolds and performance of seeded cells. Besides, the 90° FCAS significantly enhanced the compressive modulus in two groups and were more conducive to the cell proliferation and osteogenesis, which provided evidence for exploring the influence of FCAS on the properties of scaffolds and the application of two composite scaffolds in tissue regeneration.

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“…The water absorption ratio was calculated as follows: (m2 − m1)/m1 × 100. 25 All experiments were repeated three times.…”

Section: Compression Testmentioning

confidence: 99%

Silk Fibroin/Collagen/Hydroxyapatite Scaffolds Obtained by 3D Printing Technology and Loaded with Recombinant Human Erythropoietin in the Reconstruction of Alveolar Bone Defects

Liu

Wang

Sun

et al. 2022

ACS Biomater. Sci. Eng.

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The fast osteogenesis of the large alveolar fossa and the maintenance of the height of the alveolar ridge after tooth extraction have always been a clinical challenge. Therefore, this work describes the creation of innovative silk fibroin/collagen/ hydroxyapatite (SCH) biological scaffolds by 3D printing technology, which are loaded with recombinant human erythropoietin (rh-EPO) for the reconstruction of bone defects. Low-temperature 3D printing can maintain the biological activity of silk fibroin and collagen. The SCH scaffolds showed the ideal water absorption and porosity, being a sustained-release carrier of rh-EPO. The optimized scaffolds had ideal mechanical properties in vitro, and MC3T3-E1 cells could easily adhere and proliferate on it. In vivo experiments in rabbits demonstrated that the composite scaffolds gradually degraded and promoted the accumulation and proliferation of osteoblasts and the formation of collagen fibers, significantly promoting the reconstruction of mandibular defects. In this study, a novel composite biological scaffold was prepared using 3D printing technology, and the scaffold was innovatively combined with the multifunctional growth factor rh-EPO. This provides a new optimized composite material for the reconstruction of irregular mandible defects, and this biomaterial is promising for clinical reconstruction of alveolar bone defects.

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Development of novel collagen scaffolds with different bioceramic particles for bone tissue engineering

Cited by 14 publications

References 16 publications

Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Fabrication of High-Strength and Porous Hybrid Scaffolds Based on Nano-Hydroxyapatite and Human-Like Collagen for Bone Tissue Regeneration

Effects of Fiber Cross-Angle Structures on the Mechanical Property of 3D Printed Scaffolds and Performance of Seeded MC3T3-E1 Cells

Silk Fibroin/Collagen/Hydroxyapatite Scaffolds Obtained by 3D Printing Technology and Loaded with Recombinant Human Erythropoietin in the Reconstruction of Alveolar Bone Defects

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