Development of Novel Animal Model for Studying Scoliosis Using a Noninvasive Method and Its Validation through Gene-Expression Analysis

Banala, Rajkiran Reddy; Vemuri, Satish Kumar; Penkulinti, Murahari; Av, Gurava Reddy; Gpv, Subbaiah

doi:10.31616/asj.2018.0108

Cited by 3 publications

(3 citation statements)

References 21 publications

(42 reference statements)

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“…However, idiopathic scoliosis (IS) was observed in one of the MPTP-administered mice. Described as a multifactorial disease involving many intrinsic factors such as genetics, imbalance of muscle structures, abnormal growth of vertebral bodies, asymmetrical growth of the neurocentral cartilage, length discrepancy between spine and spinal cord, abnormal platelet calmodulin, and abnormality in melatonin metabolism (Banala et al, 2018;Machida, 2018); the idiopathic scoliosis observed in one of the MPTP mice may however be an epiphenomenon rather than being consequential to the MPTP administered, as has been said of many hypothesised factors (Machida, 2018).…”

Section: Discussionmentioning

confidence: 99%

Nigella Sativa Prevented Parkinson's-Like Motor Functions Impairment, Dopamine Depletion and Neuronal Degeneration in the Striatum of Mptp-Induced Balb/C Mice

Folarin

Olonade

Imam

et al. 2021

Preprint

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Background Parkinsonism is a neurological disease characterised by dopaminergic neuron degeneration in the substantial nigra and dopamine deficiency in the brain, with motor and psycho-cognitive implications, while limitations masked the efficacy of the available drugs, thus the need to find alternatives with less side effects are essential. Nigella sativa is a multi-potent plant with therapeutic potentials in the brain and other body organs. This study investigated the effects of Nigella sativa oil (NSO) on the cognitive and other Parkinsonism endophenotypes elicited by MPTP in the BALB/c strain mice. Materials and Methods Body weights, brain-body ratios, recognition memory (through novel object recognition test), as well as fronto-cortical, striatal and cerebellar dopamine and neuronal density were assayed in thirty-two (32) male BALB/c mice (18 g − 25 g). They were randomized into four groups exposed to; normal feed, 18 mg/kg MPTP i.p, 1 ml/kgbw NSO p.o., and NSO + MPTP respectively, for 5 consecutive days. Behaviours were analysed 24 hours after the last exposure, subsequently euthanized, the brains removed and processed for biomarkers analysis and histochemistry. Results Parkinsonism-like traits such as mild tremor, down-regulation of striatal and fronto-cortical dopamine and neurons were recorded in the BALB/c mice administered with MPTP only. However, significant increase (p < 0.05) in appetite, body weight, brain-body weight ratio, and recognition memory was also recorded in the MPTP-administered mice, though Nigella sativa was significantly prophylactic against the negative Parkinsonic features, and ‘moderative’ of the up-regulations induced by MPTP. Conclusion While this suggests selective MPTP sensitivity and resistance in BALB/c strains, this study recommends the investigation of possible (though ironic) beneficial potentials of MPTP.

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Section: Discussionmentioning

confidence: 99%

Nigella Sativa Prevented Parkinson's-Like Motor Functions Impairment, Dopamine Depletion and Neuronal Degeneration in the Striatum of Mptp-Induced Balb/C Mice

Folarin

Olonade

Imam

et al. 2021

Preprint

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“…Idiopathic scoliosis (IS) has been described as a multifactorial disease involving many intrinsic factors such as genetics, imbalance of muscle structures, abnormal growth of vertebral bodies, asymmetrical growth of the neurocentral cartilage, length discrepancy between spine and spinal cord, abnormal platelet calmodulin, and abnormality in melatonin metabolism (Banala, Vemuri, Penkulinti, Av, & Gpv, 2018;Machida, 2018). The idiopathic scoliosis observed in one of the MPTP mice may however be an epiphenomenon rather than being consequential to the MPTP administered, as has been said of many hypothesised factors (Machida, 2018).…”

Section: Discussionmentioning

confidence: 99%

SUB-CHRONIC MPTP-INDUCED PARKINSONISM: SELECTIVE SENSITIVITY OF THE BALB/c MICE AND THE PROPHYLACTIC ROLES OF NIGELLA SATIVA OIL

Folarin¹,

Olonade²,

Ogunkunle³

et al. 2019

Preprint

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Parkinsonism is a degenerative neurological syndrome characterised by dopamine deficiency in the basal ganglia. Despite ages of research, there is still lack of holistic and side-effect-free therapy for the disease. Nigella sativa is a multi-potent plant known for its historical and scientifically proven therapeutic potentials in the brain and other body organs. Despite the validity debates on the use of BALB/c mice strains in the modelling of Parkinsonism using MPTP, this study re-appraised the sensitivity versus resistance of the BALB/c mice strain to MPTP and further investigated the possible neuro-thrapeutic role of Nigella sativa oil (NSO) in the Parkinsonic endophenotypes elicited by MPTP in the BALB/c strain. Body weights, relative brain weights, striatal dopamine, striatal neuron density and recognition memory were studied in thirty-two (32) male albino mice, weighing between 18g -25g. They were divided equally into Control (administered with normal feed for 5 days), MPTP (administered with 18mg/kg MPTP i.p for 5 days), NS (administered with 1ml/kgbw NSO p.o. for 5 days), and NS+MPTP (administered with 1ml/kgbw NSO p.o. followed by 18mg/kg MPTP i.p for 5 days). Recognition memory was assayed through Novel Object Recognition test (NORT), and the animals were weighed and euthanised 24 hours after last administration. The brains were excised and the striatum assayed neurochemically for dopamine and illustrated histologically for neuronal density using the H&E stain. Parkinsonic traits such as mild tremor, significant down-regulation of dopamine and striatal neurons (p<0.05) were recorded in the BALB/c mice administered with MPTP only, confirming MPTP-sensitivity for these features. However, significant increase (p<0.05) in appetite, body weight, brain-body weight ratio, and recognition memory was also recorded in the MPTP-administered mice, though Nigella sativa was significantly prophylactic against the negative Parkinsonic features, and ‘moderative’ of the up-regulations induced by MPTP. While this suggests selective MPTP sensitivity and resistance in BALB/c strains, this study recommends the investigation of possible beneficial potentials of MPTP as observed. The research was conducted in conformance with the Animal Research Ethics Committee (AREC) guidelines of the Olabisi Onabanjo University.

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“…The idiopathic scoliosis (IS) was considered as a multifactorial disease, which was been proposed attributed to a number of factors, such as genetic, biochemical, and hormonal factors [1][2][3][4]. In addition, asymmetric loading on the spinal column may play roles in the onset and development of IS.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Pathological Role of Collagen in Paravertebral Muscle in the Progression of Idiopathic Scoliosis

Peng

Jin

Meng

et al. 2020

BioMed Research International

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Background. Paravertebral muscle (PVM) is considered as a contributing factor of idiopathic scoliosis (IS); collagen is crucial for maintaining the mechanical properties of PVM, but only a few researches have described this field. In this study, we observed the muscle stiffness of PVM and the curvature of the spine by adjusting the content of collagen in PVM of rats and explored the role of collagen in the progression of IS. Methods. 32 female Sprague Dawley rats were randomly divided into four groups: neutralizing antibody (NA) group (group 1), normal control group (group 2), IS group (group 3), and IS with NA group (group 4). TGF-β1 NA was injected into PVM in group 1 and group 4, while Normal saline in group 2 and group 3. The Cobb angle and muscle stiffness were measured before and after injection; the rats were sacrificed at one week after injection, and performed histological, Western Blot, and qRT-PCR examinations. Results. X-rays showed that scoliosis occurred in group 1 and relieved in group 4. The stiffness of PVM was decreased significantly on the convex side in group 1, while on the concave side in group 4. The expression of TGF-β1 and COL1 on the concave side in IS rats (group 3) was significantly increased than that in normal rats (group 2), the concentration of COL1 and COL3 in group 3 was significantly higher than that in group 2, and the addition of TGF-β1 NA significantly downregulated COL1 and COL3 in group 1 and group 4. The concentration of COL1 in convex PVM was negatively related to Cobb angle in group 1 and group 2, and in concave PVM was positively related to Cobb angle in group 3 and group 4. However, no significant correlation was found between COL3 and Cobb angle in group 3 and group 4. Conclusions. Asymmetric biomechanical characteristics of PVM was an important etiological factor of IS, which was directly correlated with collagen, it could be adjusted by local intramuscular injecting of TGF-β1 NA, and finally had an effect on the shape of the spine.

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Development of Novel Animal Model for Studying Scoliosis Using a Noninvasive Method and Its Validation through Gene-Expression Analysis

Cited by 3 publications

References 21 publications

Nigella Sativa Prevented Parkinson's-Like Motor Functions Impairment, Dopamine Depletion and Neuronal Degeneration in the Striatum of Mptp-Induced Balb/C Mice

Nigella Sativa Prevented Parkinson's-Like Motor Functions Impairment, Dopamine Depletion and Neuronal Degeneration in the Striatum of Mptp-Induced Balb/C Mice

SUB-CHRONIC MPTP-INDUCED PARKINSONISM: SELECTIVE SENSITIVITY OF THE BALB/c MICE AND THE PROPHYLACTIC ROLES OF NIGELLA SATIVA OIL

Exploring the Pathological Role of Collagen in Paravertebral Muscle in the Progression of Idiopathic Scoliosis

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