Development of new PCR primers for identification of <i>Monilinia</i> species*

Hughes, Kelvin; Fulton, C. E.; McReynolds, D.; Lane, Charles R.

doi:10.1111/j.1365-2338.2000.tb00938.x

Cited by 37 publications

(55 citation statements)

References 7 publications

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“…Molecular-genetic identification was carried out by polymerase chain reaction (PCR). DNA was isolated by standard isolation kits and specific sets of PCR primers according to Ioos and Frey (2000) for M. fructicola and Hughes et al (2000) for M. laxa and M. fructigena were used.…”

Section: Methodsmentioning

confidence: 99%

First report on Monilinia fructicola in the Slovak Republic

Ondejková¹,

Hudecová²,

Bacigálová³

2010

Plant Prot. Sci.

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Ondejková N., Hudecová M., Bacigálová K. (2010): First report on Monilinia fructicola in the Slovak Republic. Plant Protect Sci., 46: 181-184.The occurrence of Monilinia on stone and pome fruit trees in Slovakia was studied. Commonly distributed species Monilinia laxa, M. fructigena, and American species M. fructicola were determined by the methods used. M. fructicola was identified on the fruits of peach and nectarine imported to our country and on domestic plum fruits as well. To our knowledge, this is the first report on M. fructicola in Slovakia and a new member for Slovak mycobiota.

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Section: Methodsmentioning

confidence: 99%

First report on Monilinia fructicola in the Slovak Republic

Ondejková¹,

Hudecová²,

Bacigálová³

2010

Plant Prot. Sci.

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“…A single colony was stored on potato dextrose agar plates at 4°C. Molecular analysis according to Hughes et al (2000) suggested that the isolate corresponded to M. fructigena (not shown). Mycelial plugs were used to infect previously disinfected peach fruits, in order to generate abundant spores for artificial inoculations.…”

Section: Plant Materialsmentioning

confidence: 99%

QTL mapping for brown rot (Monilinia fructigena) resistance in an intraspecific peach (Prunus persica L. Batsch) F1 progeny

Pacheco

Bassi

Eduardo

et al. 2014

Tree Genetics & Genomes

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Brown rot (BR) caused by Monilinia spp. leads to significant post-harvest losses in stone fruit production, especially peach. Previous genetic analyses in peach progenies suggested that BR resistance segregates as a quantitative trait. In order to uncover genomic regions associated with this trait and identify molecular markers for assisted selection (MAS) in peach, an F1 progeny from the cross "Contender" (C, resistant)×"Elegant Lady" (EL, susceptible) was chosen for quantitative trait loci (QTL) analysis. Over two phenotyping seasons, skin (SK) and flesh (FL) artificial infections were performed on fruits using a Monilinia fructigena isolate. For each treatment, infection frequency (if) and average rot diameter (rd) were scored. Significant seasonal and intertrait correlations were found. Maturity date (MD) was significantly correlated with disease impact. Sixty-three simple sequence repeats (SSRs) plus 26 single-nucleotide polymorphism (SNP) markers were used to genotype the C×EL population and to construct a linkage map. C×EL map included the eight Prunus linkage groups (LG), spanning 572.92 cM, with an average interval distance of 6.9 cM, covering 78.73 % of the peach genome (V1.0). Multiple QTL mapping analysis including MD trait as covariate uncovered three genomic regions associated with BR resistance in the two phenotyping seasons: one containing QTLs for SK resistance traits near M1a (LG C× EL-2, R 2 =13.1-31.5 %) and EPPISF032 (LG C×EL-4, R 2 = 11-14 %) and the others containing QTLs for FL resistance, near markers SNP_IGA_320761 and SNP_IGA_321601 (LG3, R 2 =3.0-11.0 %). These results suggest that in the C× EL F1 progeny, skin resistance to fungal penetration and flesh resistance to rot spread are distinguishable mechanisms constituting BR resistance trait, associated with different genomic regions. Discovered QTLs and their associated markers could assist selection of new cultivars with enhanced resistance to Monilinia spp. in fruit.

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“…Standard methods for precise identification of Monilinia species include: investigation of morphological and cultural features (Byrde and Willetts, 1977;De Cal and Melgarejo, 1999) and/or molecular methods (Hughes et al, 2000;Còté et al, 2004).…”

Section: Identification Of Monilinia Speciesmentioning

confidence: 99%

Genus Monilinia on pome and stone fruit species

Hrustić

Mihajlović

Grahovac

et al. 2012

Pesticidi i fitomedicina

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SUMMARYDifferent species of the genus Monilinia are common plant pathogens that endanger pome and stone fruit production worldwide. In Serbia, two species of this genus are widely distributed -M. laxa and M. fructigena, while M. fructicola, which is officially on the A2 EPPO List of quarantine pest organisms in Europe and on the 1A part I List of quarantine pest organisms in Serbia, has so far been detected only on stored apple and nectarine fruits. The most important control measures against these pathogens include chemical control in combination with adequate cultural practices, particularly under favourable conditions for disease development. Concerning that species of this genus can cause significant economic losses, knowledge of the pathogen biology, disease epidemiology and pathogen-host interactions is a necessary prerequisite for stable and profitable production of pome and stone fruits.

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Development of new PCR primers for identification of Monilinia species*

Cited by 37 publications

References 7 publications

First report on Monilinia fructicola in the Slovak Republic

First report on Monilinia fructicola in the Slovak Republic

QTL mapping for brown rot (Monilinia fructigena) resistance in an intraspecific peach (Prunus persica L. Batsch) F1 progeny

Genus Monilinia on pome and stone fruit species

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