Development of New Method for Simultaneous Analysis of Piracetam and Levetiracetam in Pharmaceuticals and Biological Fluids: Application in Stability Studies

Siddiqui, Farhan Ahmed; Sher, Nawab; Shafi, Nighat; Sial, Alisha Wafa; Ahmad, Mansoor; Mehjebeen,; Naseem, Huma

doi:10.1155/2014/758283

Cited by 19 publications

(15 citation statements)

References 20 publications

(9 reference statements)

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“…The T max (0.87 h) of piracetam was 2.5 times less than the value reported in the literature ( T max 2.33 h) for the same (800 mg) dose (Barkat et al ., ). The plasma concentrations of both studies were quantified by HPLC, the volunteers were Pakistani, and the reference formulation (Ceremin) was different (Barkat et al ., ; Siddiqui et al ., ). These factors may explain the observed differences in plasma concentration.…”

Section: Resultsmentioning

confidence: 95%

“…After oral administration of the piracetam tablet (800 mg) to the volunteers, the peak plasma concentrations ( C max 28.96 µg/mL) of piracetam were 2 ( C max 14.53 µg/mL) and 3 ( C max 8 µg/mL) times higher than the values reported in the literature for the same dose (800 mg; Barkat et al ., ; Siddiqui et al ., ). The T max (0.87 h) of piracetam was 2.5 times less than the value reported in the literature ( T max 2.33 h) for the same (800 mg) dose (Barkat et al ., ).…”

Section: Resultsmentioning

confidence: 99%

“…This is the first LC-MS-MS method developed for measuring piracetam in human plasma. Piracetam has been determined in human plasma (LOQ 0.01-3 μg/mL, retention time 5.1-6 min, liquid-liquid extraction; Barkat et al, 2014;Curticapean and Imre, 2007;Louchahi et al, 1995;Siddiqui et al, 2014), in pharmaceutical syrup (LOQ 0.01 μg/mL, retention time 6 min, liquid-liquid extraction; Siddiqui et al, 2014) and urine (LOQ 100 μg/mL, liquid-liquid extraction) by HPLC. Piracetam has been determined in rat plasma (LOQ 0.1 μg/mL, retention time 1.8 min, liquid-liquid extraction, internal standard oxiracetam) by LC-MS-MS (Barkat et al, 2014).…”

Section: Bioanalytical Performancementioning

confidence: 99%

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Assessment of pharmacokinetic interaction between piracetam and l‐carnitine in healthy subjects

Mendes

Zaffalon

Silveira³

et al. 2015

Biomedical Chromatography

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A rapid, sensitive and specific method for quantifying piracetam in human plasma using Piracetam d-8 as the internal standard (IS) is described. The analyte and the IS were extracted from plasma by one-step precipitation of protein using an acetonitrile (100%). The extracts were analyzed by high-performance liquid chromatography coupled with electrospray tandem mass spectrometry (HPLC-MS/MS). The method had a chromatographic run time of 3.8 min and a linear calibration curve over the range 0.5-50 µg/mL (r > 0.99). This LC-MS-MS procedure was used to assess the bioavailability of two piracetam formulations: piracetam + l-carnitine (Piracar®; 270/330 mg tablet) and piracetam (Nootropil®; 800 mg tablet) in healthy volunteers of both sexes. The geometric means with corresponding 90% confidence interval (CI) for test/reference percentage ratios were 88.49% (90% CI = 81.19 - 96.46) for peak concentration/dose and 102.55% (90% CI = 100.62 - 104.51) for AUCinf /dose. The limit of quantitation of 0.5 µg/mL is well suited for pharmacokinetic studies in healthy volunteers. It was concluded that piracetam (Piracar®; 270/330 mg tablet) has a bioavailability equivalent to the piracetam (Nootropil®; 800 mg tablet) formulation with regard to both the rate and the extent of absorption.

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 99%

Section: Bioanalytical Performancementioning

confidence: 99%

See 1 more Smart Citation

Assessment of pharmacokinetic interaction between piracetam and l‐carnitine in healthy subjects

Mendes

Zaffalon

Silveira³

et al. 2015

Biomedical Chromatography

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“…The extensive literature survey revealed few methods are developed to estimate the drug Levetiracetam in raw material, tablets and in biological fluids using UV [5][6][7], RP-HPLC [2,[8][9][10][11][12][13][14][15][16][17][18], LC-MS [19][20][21], Capillary electrophoresis [22], UPLC [23,24], Gas Chromatography [25,26] and HPTLC [27]. However, there is no method developed yet for the simultaneous estimation of Levetiracetam and its preservatives by RP-HPLC in oral liquid dosage forms.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Estimation of Levetiracetam and its Preservatives In Oral Liquid Dosage form by Rp-Hplc Method

Grace¹,

Prabha²,

Jagadeeswaran³

et al. 2016

SOJMSE

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A rapid, simple, sensitive and accurate RP-HPLC method was developed and validated for simultaneous estimation of Levetiracetam and its preservatives (Methyl paraben and Propyl paraben) in oral liquid dosage form. The chromatographic separations were achieved on a Zorbax CN column (250×4.6 mm inner diameter, 5µm) using a mobile phase consisting of orthophosphoric acid buffer (pH 4.6)acetonitrile (75:25 v/v) at a flow rate of 1.0 mL/min with UV detection at 210nm. This system produced sharp peaks with good resolution, minimum tailing and satisfactory retention time for Levetiracetam, Methyl paraben and Propyl paraben were found to be 4.601, 9.905 and 19.291 respectively. The method was validated as per USP guidelines which include accuracy, precision, linearity and range, robustness specificity and ruggedness. The described method was linear over the range of 30-90 µg/ml for Levetiracetam with r² of 0.9999 and 60-180 µg/ml for Methyl paraben with r² 0.9997 and 20-60 µg/ml for Propyl paraben with r² 0.9999. The average recovery of the method was 98.2%, 99.3% and 98.7% for Levetiracetam, Methyl paraben and Propyl paraben respectively. The developed method is repeatable and selective for the analysis of Levetiracetam and its preservatives in oral liquid dosage form. Hence the method could be successfully applied for routine analysis. The developed method could also applicable in quality control testing in analysis of oral liquid dosage form and in process testing.

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“…British pharmacopoeia states a chromatographic method for piracetam determination. Several methods have been developed for piracetam determination, including spectrophotometric methds [14][15][16][17], high-performance liquid chromatography (HPLC) [18][19][20][21][22] and electrochemical method [23].…”

Section: Introductionmentioning

confidence: 99%

Exploring Novel Isocractic HPLC Method for Quantitative Determination of Cinnarizine and Piracetam in Their Capsule Preparations

Sm¹,

Me²,

Mh³

2016

J App Pharm

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A novel isocratic HPLC method had been developed for rapid simultaneous separation and determination of cinnarizine and Piracetam in pure form or in pharmaceutical formulations within less than 10 minutes. Separation was carried out on a Hypersil gold C 18 (10µm, 100x4.6mm) column. Effect of pH and composition of mobile phase in addition to flow rate was studied. Calibration was obeyed in the range of (10-80) µg/ml for cinnarizine or (160-960) µg/ ml for Piracetam. The method was applied for the simultaneous determination of these drugs in both bulk and pharmaceutical forms and this is the first published method that uses isocratic HPLC method for cinnarizine and piracetam determination in combined formulation and the method were validated according to ICH parameters.

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Development of New Method for Simultaneous Analysis of Piracetam and Levetiracetam in Pharmaceuticals and Biological Fluids: Application in Stability Studies

Cited by 19 publications

References 20 publications

Assessment of pharmacokinetic interaction between piracetam and l‐carnitine in healthy subjects

Assessment of pharmacokinetic interaction between piracetam and l‐carnitine in healthy subjects

Simultaneous Estimation of Levetiracetam and its Preservatives In Oral Liquid Dosage form by Rp-Hplc Method

Exploring Novel Isocractic HPLC Method for Quantitative Determination of Cinnarizine and Piracetam in Their Capsule Preparations

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