Development of Near-Isogenic Line of japonica Rice Cultivar Saenuri without Lipoxygenase-3

Abstract

It is reported that the absence of lipoxygenase-3 (LOX-3) may contribute to a reduction in stale flavor after the storage of rice.To improve the quality of stored rice of the Korean japonica rice cultivar, we conducted a breeding program to develop near-isogenic rice without LOX-3 in the genetic background of Saenuri, a mega variety of Korea. In the first step of the breeding program, we used a donor parent of LOX-3 null, Daw Dam, and a recurrent japonica parent, Sindongjin, to develop HR27873-AC12 by backcross (BC1), color test for introgression of lox-3, and anther culture for rapid fixation. In the second step, we used the donor parent, HR27873-AC12, and the recurrent parent, Saenuri, to develop HR28896-31-3-1-1 by backcross (BC1), marker-assisted selection (MAS) for lox-3, and phenotypic selection (PS) for agronomic traits. Finally, in the third step, we developed HR30960-186-2-1-2-1 (Jeonju624), derived from a cross between Saenuri and HR28896-31-3-1-1, by MAS for lox-3 and PS with high selection pressure for agronomic characteristics. Jeonju624 was confirmed with the introgression of lox-3 by molecular marker. Jeonju624 was a mid-late maturing rice with similar agronomic characteristics to Saenuri, lodging tolerance with short culm, erect plant architecture, and resistance to bacterial blight and rice stripe virus. The yield components of Jeonju624 were mostly similar to Saenuri, except for the 1,000-grain weight of brown rice. The appearance of the grain of Jeonju624 was better than that of Saenuri, and the characteristics of cooked rice were similar to those of Saenuri. In the genetic background analysis using 406 KASP (Kompetitive Allele-Specific PCR) markers, Jeonju624 was confirmed to be the near-isogenic line (NIL) of Saenuri with a 95.8% recovery rate. Jeonju624 is the NIL of Saenuri without LOX-3, and overcomes the linkage drag of Daw Dam with similar agronomic characteristics and genetic background to Saenuri. Jeonju624 can be utilized as a practical cultivar to improve the quality of stored rice, breeding material for the introgression of lox-3, and genetic material to elucidate the effect of introgressed genes.

Marker-Assisted Backcrossing (MABc) to Improve Eating Quality with Thin Seed Coat and Aleurone Layer of Non-Glutinous Japonica Variety in Rice

“…Among 386 KASP markers showing polymorphism, markers showing hetero genotypes were excluded, and 96 markers located at 5 Mb intervals per 12 chromosomes in rice were selected to analyze the recurrent parent genome recovery ratios in BC 1 F 1 and BC 2 F 1 of Samgwang/Seolgaeng. A genetic graphic map was derived using a MapChart program (version 2.32) based on the physical locations of the KASP markers on chromosomes [ 31 ].…”

Breeding of High Cooking and Eating Quality in Rice by Marker-Assisted Backcrossing (MABc) Using KASP Markers

“…Ninety-six markers located at a 5 Mb intervals on 12 rice chromosomes were selected to analyze the recovery ratio of the recurrent parent genome in BC 1 F 1 and BC 2 F 1 . Genetic graphical mapping was performed using a MapChart program (version 2.32) based on the physical location of the KASP markers on chromosomes [ 61 ]. The agronomic traits such as plant height, culm length, panicle length, and the number of tillers were phenotyped based on a standard method of RDA, Korea [ 56 ] in the lines selected in the BC 1 F 1 and BC 2 F 1 generations.…”

Development of 454 New Kompetitive Allele-Specific PCR (KASP) Markers for Temperate japonica Rice Varieties

“…Unkwang was crossed to RSV-resistant variety Haedamssal as the donor of the RSV resistance gene, and the genetic backgrounds of BC 2 F 1 and BC 2 F 2 plants were analyzed with KASP markers to enable selection of a NIL with 96.2% recovery of the recurrent parent genome [ 22 ]. In a separate program to breed rice with reduced stale flavors after storage, 406 KASP markers were used for genetic background analysis of Jeonju624, a NIL of Korean temperate japonica rice variety Saenuri with a null allele of lipoxygenase-3 introduced from donor line HR27873-AC12, and revealed 95.8% recovery of the recurrent parent genome [ 23 ].…”