Development of nanofibrous scaffolds by varying the TiO<sub>2</sub> content in crosslinked PVA for bone tissue engineering

Pattanashetti, Nandini A.; Hiremath, Chinmay G.; Naik, Satishkumar R.; Heggannavar, Geetha B.; Kariduraganavar, Mahadevappa Y.

doi:10.1039/c9nj05118j

Cited by 26 publications

(8 citation statements)

References 31 publications

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“…102 To overcome this shortage, Kariduraganavar and co-workers crosslinked PVA with the minimum ratio of non-toxic tetraethylorthosilicate to enhance the stability of electrospun nanofibers in aqueous media. 107 Bonakdar et al modified the PVA composite scaffolds via incorporating HAp as well as cellulose nanofibers, and obtained a 7-fold increase of mechanical strength. 102 More than that, PVP is often used for the hydrophilic modification of other synthetic polymers for bone regeneration.…”

Section: Electrospun Nanofibers For Bone Regenerationmentioning

confidence: 99%

Electrospun nanofibers for bone regeneration: from biomimetic composition, structure to function

et al. 2022

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Section: Electrospun Nanofibers For Bone Regenerationmentioning

confidence: 99%

Electrospun nanofibers for bone regeneration: from biomimetic composition, structure to function

et al. 2022

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“…The particle per unit space is more in the SPI-TiO 2 (1%) composites, hence it slightly decreases the space for proper growth. However, the distribution of the microfiller is not so uniform that still there is enough space for the cells for healthy living and proliferation. , The results show that the viability of the cells for all membranes prepared in this experiment was excellent on the whole, showing no cytotoxicity.…”

Section: Resultsmentioning

confidence: 73%

Tissue Engineering Scaffold Material with Enhanced Cell Adhesion and Angiogenesis from Soy Protein Isolate Loaded with Bio Modulated Micro-TiO₂ Prepared via Prolonged Sonication for Wound Healing Applications

Koshy

Mary

Thomas³

et al. 2022

ACS Biomater. Sci. Eng.

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Tissue engineering is a technique that promotes healing by creating an ideal environment for endogenous cells to migrate and grow into the site of injury via a scaffold, improving regeneration and reducing the time required for in vitro cell culture. In this work, the effect of the addition of sonicated TiO 2 in the soy protein isolate (SPI) matrix for tissue engineering applications was studied. In comparison to adding expensive nano TiO 2 , this method of incorporating sonicated TiO 2 into the SPI matrix will aid in achieving improved properties at a lower cost. The effect of the addition of sonicated TiO 2 on the morphological, UV transmittance, mechanical, thermal, surface energy, and hydrophilicity of SPI films was investigated. The result shows that the uniformly distributed TiO 2 particles successfully blocked 95% of UV light. Scanning electron microscopy revealed a significant reduction in the TiO 2 agglomerate size and homogeneous distribution of the same when sonication was applied instead of mechanical dispersion. A simultaneous increase of tensile strength (from 3.16 to 4.58 MPa) and elongation at break values (from 24.25% to 95.31%) with 0.5% TiO 2 was observed. The addition of 0.25% TiO 2 was found to significantly enhance the elongation at break value to 120.83%. Incorporation of micro-TiO 2 particles could improve the surface roughness, surface energy, and wettability of SPI films. In vitro cell adhesion studies and in vivo subcutaneous implantation studies were performed to assess the cell growth and angiogenesis of the developed film membranes. An MTT assay showed that SPI-1%TiO 2 film favored cell viability up to 118%, and in vivo subcutaneous implantation studies showed enhanced cell growth and angiogenesis for SPI-1% TiO 2 films. This SPI-TiO 2 film with enhanced surface properties can be used as an ideal candidate for tissue engineering applications.

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“…The PCL/gelatin compound fiber membrane is hydrophilic because the gelatin structure contains amine and carboxyl functional groups, which contribute to various functional applications of gelatin including pharmaceuticals, food, and biomedicine. [ 27 ] The mean value of P8G2 and P8G2‐C was 53.0 ± 4.5° and 57.1 ± 0.5°. The mean values of P5G5 and P5G5‐C were 64.2 ± 2.1° and 57.4 ± 0.7°.…”

Section: Resultsmentioning

confidence: 99%

Opening the Soul Window Manually: Limbal Tissue Scaffolds with Electrospun Polycaprolactone/Gelatin Nanocomposites

Wang

Gao

et al. 2020

Macromolecular Bioscience

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Restricted by the difficulty in fabricating scaffolds suitable for cell proliferation, the use of ex vivo expanded limbal stem cell (LSC) for LSC transplantation, an effective treatment method for patients with limb stem cell deficiency (LSCD), is hard to be widely used in clinical practice. To tackle these challenges, a novel electrospun polycaprolactone (PCL)/gelatin nanocomposite is proposed to make 3D scaffolds for limbal niche cells (LNC) proliferation in vitro, which is a milestone in the treatment of diseases such as LSCD. PCL and gelatin in different weight ratios are dissolved in a mixed solvent, and then electrospinning and cross‐linking are performed to prepare a scaffold for cell proliferation. The characterizations of the nanocomposites indicate that the gelatin content has a significant effect on its micro‐morphology, thermal properties, crystallinity, degradation temperature, hydrophilicity, and mechanical properties. P8G2‐C (PCL: gelatin = 80: 20, cross‐linked), with smooth fibers and homogeneous pores, has better hydrophilicity, mechanical properties, and flexibility, so it can support LNC as cell proliferation assays revealed. This detailed investigation presented here demonstrates the feasibility of using PCL/gelatin nanocomposites electrospun fiber membranes as a limbus tissue engineering scaffold, which undoubtedly provide a new perspective for the development of tissue engineering field.

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Development of nanofibrous scaffolds by varying the TiO₂ content in crosslinked PVA for bone tissue engineering

Abstract: Development of TiO2 incorporated crosslinked PVA scaffolds with required characteristics for bone tissue engineering.

Cited by 26 publications

References 31 publications

Electrospun nanofibers for bone regeneration: from biomimetic composition, structure to function

Electrospun nanofibers for bone regeneration: from biomimetic composition, structure to function

Tissue Engineering Scaffold Material with Enhanced Cell Adhesion and Angiogenesis from Soy Protein Isolate Loaded with Bio Modulated Micro-TiO₂ Prepared via Prolonged Sonication for Wound Healing Applications

Opening the Soul Window Manually: Limbal Tissue Scaffolds with Electrospun Polycaprolactone/Gelatin Nanocomposites

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Development of nanofibrous scaffolds by varying the TiO2 content in crosslinked PVA for bone tissue engineering

Abstract: Development of TiO2 incorporated crosslinked PVA scaffolds with required characteristics for bone tissue engineering.

Cited by 26 publications

References 31 publications

Electrospun nanofibers for bone regeneration: from biomimetic composition, structure to function

Electrospun nanofibers for bone regeneration: from biomimetic composition, structure to function

Tissue Engineering Scaffold Material with Enhanced Cell Adhesion and Angiogenesis from Soy Protein Isolate Loaded with Bio Modulated Micro-TiO2 Prepared via Prolonged Sonication for Wound Healing Applications

Opening the Soul Window Manually: Limbal Tissue Scaffolds with Electrospun Polycaprolactone/Gelatin Nanocomposites

Contact Info

Product

Resources

About

Development of nanofibrous scaffolds by varying the TiO₂ content in crosslinked PVA for bone tissue engineering

Tissue Engineering Scaffold Material with Enhanced Cell Adhesion and Angiogenesis from Soy Protein Isolate Loaded with Bio Modulated Micro-TiO₂ Prepared via Prolonged Sonication for Wound Healing Applications