Development of nanocellulose scaffolds with tunable structures to support 3D cell culture

Liu, Jun; Cheng, Fang; Grénman, Henrik; Spoljaric, Steven; Seppälä, Jukka; Eriksson, John E.; Willför, Stefan; Xu, Chunlin

doi:10.1016/j.carbpol.2016.04.064

Cited by 129 publications

(91 citation statements)

References 52 publications

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“…26 Nanocellulose-based foams and aerogels have been shown to support growth and proliferation of cells. 94,97 Using 3D TEMPO-CNF aerogel scaffolds for cell cultures resulted in less than 5% of cell death aer 72 h of cell growth. 97 An even lower cell death was observed when low charge density TEMPO-CNF (1.56 mmol g À1 against 2.04 mmol g À1 ) was used to form the scaffolds.…”

Section: Scaffolds For Biomedical and Pharmaceutical Applicationsmentioning

confidence: 99%

Nanocellulose-based foams and aerogels: processing, properties, and applications

2017

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Section: Scaffolds For Biomedical and Pharmaceutical Applicationsmentioning

confidence: 99%

Nanocellulose-based foams and aerogels: processing, properties, and applications

2017

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“…Pore diameter and distribution or fiber size and alignment are often analyzed by randomly picking at least dozens of various segments by means of image J software [65,109]. Individual scaffold dimensions can be measured with digital calipers [108,110]. Scaffold porosity can be determined using liquid displacement method [104].…”

Section: Morphology and Structurementioning

confidence: 99%

Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

et al. 2020

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Poly(N-isopropylacrylamide) (PNIPAAm) is a typical thermoresponsive polymer used widely and studied deeply in smart materials, which is attractive and valuable owing to its reversible and remote "on-off" behavior adjusted by temperature variation. PNIPAAm usually exhibits opposite solubility or wettability across lower critical solution temperature (LCST), and it is readily functionalized making it available in extensive applications. Cell culture is one of the most prospective and representative applications. Active attachment and spontaneous detachment of targeted cells are easily tunable by surface wettability changes and volume phase transitions of PNIPAAm modified substrates with respect to ambient temperature. The thermoresponsive culture platforms and matching thermal-liftoff method can effectively substitute for the traditional cell harvesting ways like enzymatic hydrolysis and mechanical scraping, and will improve the stable and high quality of recovered cells. Therefore, the establishment and detection on PNIPAAm based culture systems are of particular importance. This review covers the important developments and recommendations for future work of the preparation and characterization of temperature-responsive substrates based on PNIPAAm and analogues for cell culture applications.

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“…On the contrary, MOAs with long aspect ratio fibers render a larger amount of empty areas, thus preventing breakage and conferring plasticity upon material. Specific compressive strength measured for the toughest MOAs (NiDTA‐100: 12725 J kg −1 ; NiDTA‐50: 1662 J kg −1 ; and PdDTA: 258 J kg −1 ) is far higher than that found for the unique MOF‐aerogel mechanically characterized, and somewhat higher than many carbon, organic, ceramic, and metal aerogels, and comparable to some conventional polymeric foams like polystyrene, polyurethane, etc. (Figure b).…”

Section: Resultsmentioning

confidence: 81%

Chemically Resistant, Shapeable, and Conducting Metal‐Organic Gels and Aerogels Built from Dithiooxamidato Ligand

Vallejo-Sánchez

Amo‐Ochoa

Beobide

et al. 2017

Adv Funct Materials

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Metal‐organic gels (MOGs) appear as a blooming alternative to well‐known metal‐organic frameworks (MOFs). Porosity of MOGs has a microstructural origin and not strictly crystalline like in MOFs; therefore, gelation may provide porosity to any metal‐organic system, including those with interesting properties but without a porous crystalline structure. The easy and straightforward shaping of MOGs contrasts with the need of binders for MOFs. In this contribution, a series of MOGs based on the assembly of 1D‐coordination polymer nanofibers of formula [M(DTA)]n (MII: Ni, Cu, Pd; DTA: dithiooxamidato) are reported, in which properties such as porosity, chemical inertness, mechanical robustness, and stimuli‐responsive electrical conductivity are brought together. The strength of the MS bond confers an unusual chemical resistance, withstanding exposure to acids, alkalis, and mild oxidizing/reducing chemicals. Supercritical drying of MOGs provides ultralight metal‐organic aerogels (MOAs) with densities as low as 0.03 g cm−3 and plastic/brittle behavior depending on the nanofiber aspect ratio. Conductivity measurements reveal a semiconducting behavior (10−12 to 10−7 S cm−1 at 298 K) that can be improved by doping (10−5 S cm−1). Moreover, it must be stressed that conductivity of MOAs reversibly increases (up to 10−5 S cm−1) under the presence of acetic acid.

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Development of nanocellulose scaffolds with tunable structures to support 3D cell culture

Cited by 129 publications

References 52 publications

Nanocellulose-based foams and aerogels: processing, properties, and applications

Nanocellulose-based foams and aerogels: processing, properties, and applications

Preparation and Characterization of Thermoresponsive Poly(N-Isopropylacrylamide) for Cell Culture Applications

Chemically Resistant, Shapeable, and Conducting Metal‐Organic Gels and Aerogels Built from Dithiooxamidato Ligand

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