Development of mycoinsecticide formulations with Beauveria bassiana and Metarhizium anisopliae for the control of lesser mealworm, Alphitobius diaperinus, in chicken broiler houses

Rice, Steven J.; Baker, Dalton K.; Leemon, Diana M.

doi:10.1007/s10526-019-09951-3

Cited by 13 publications

(5 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…vuill. (1912) [ 16 ] and Metarhizium anisopliae (Metchnikoff) Sorokin (1883) have shown potential against various pests, including Aedes albopictus (Skuse, 1894) [ 17 ], Bemisia tabaci (Gennadius, 1889) [ 18 ], Spodoptera frugiperda (J. E. Smith, 1797) [ 19 ], Locusta migratoria (Linnaeus, 1758) [ 20 ], Alphitobius diaperinus (Panzer, 1797) [ 21 ], Nilaparvata lugens (Stål, 1854) [ 22 ], and Plutella xylostella (Linnaeus, 1758) [ 23 ]. However, the effectiveness of these biopesticides is often limited by the defense systems of insect pests [ 24 ].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Transcriptomic Analysis Reveals the Impact of the Biopesticide Metarhizium anisopliae on the Immune System of Major Workers in Solenopsis invicta

Zafar

et al. 2023

Insects

View full text Add to dashboard Cite

The red imported fire ant (Solenopsis invicta Buren, 1972) is a globally significant invasive species, causing extensive agricultural, human health, and biodiversity damage amounting to billions of dollars worldwide. The pathogenic fungus Metarhizium anisopliae (Metchnikoff) Sorokin (1883), widely distributed in natural environments, has been used to control S. invicta populations. However, the interaction between M. anisopliae and the immune system of the social insect S. invicta remains poorly understood. In this study, we employed RNA-seq to investigate the effects of M. anisopliae on the immune systems of S. invicta at different time points (0, 6, 24, and 48 h). A total of 1313 differentially expressed genes (DEGs) were identified and classified into 12 expression profiles using short time-series expression miner (STEM) for analysis. Weighted gene co-expression network analysis (WGCNA) was employed to partition all genes into 21 gene modules. Upon analyzing the statistically significant WGCNA model and conducting Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis on the modules, we identified key immune pathways, including the Toll and Imd signaling pathways, lysosomes, autophagy, and phagosomes, which may collectively contribute to S. invicta defense against M. anisopliae infection. Subsequently, we conducted a comprehensive scan of all differentially expressed genes and identified 33 immune-related genes, encompassing various aspects such as recognition, signal transduction, and effector gene expression. Furthermore, by integrating the significant gene modules derived from the WGCNA analysis, we constructed illustrative pathway diagrams depicting the Toll and Imd signaling pathways. Overall, our research findings demonstrated that M. anisopliae suppressed the immune response of S. invicta during the early stages while stimulating its immune response at later stages, making it a potential biopesticide for controlling S. invicta populations. These discoveries lay the foundation for further understanding the immune mechanisms of S. invicta and the molecular mechanisms underlying its response to M. anisopliae.

show abstract

Section: Introductionmentioning

confidence: 99%

“…Although much work has been performed on insect-pathogen interaction, little is known about the immune defense mechanism of S. invicta [ 21 , 29 , 30 ]. The present study aims to elucidate the immune responses of S. invicta during M. anisopliae by high-throughput RNA-seq analysis.…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic Analysis Reveals the Impact of the Biopesticide Metarhizium anisopliae on the Immune System of Major Workers in Solenopsis invicta

Zafar

et al. 2023

Insects

View full text Add to dashboard Cite

show abstract

“…Compared to chemical insecticides, the fungal pesticides are environmentally friendly biocontrol agents with less risks for nontarget organisms [ 10 ]. Some entomopathogenic fungi, such as Metarhizium anisopliae and Beauveria bassiana , have been used for the control of agricultural and forestry pests, including Chironomus riparius [ 11 ], Locusta migratoria [ 12 ], Helicoverpa armigera [ 13 ], Alphitobius diaperinus [ 14 ] and Nilaparvata lugens [ 15 , 16 ]. Combined use of M. anisopliae and dsRNA or chemicals has shown good potential for the control of the rice pest N. lugens [ 16 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

The Entomopathogenic Fungus Metarhizium anisopliae Affects Feeding Preference of Sogatella furcifera and Its Potential Targets’ Identification

Wang

Han

Xia

et al. 2022

JoF

View full text Add to dashboard Cite

The rice planthopper Sogatella furcifera is a unique vector of the southern rice black-streaked dwarf virus (SRBSDV). The feeding behavior of S. furcifera should directly affect the diffusion of this virus. In this study, we noted that the infection of Metarhizium anisopliae CQMa421 on S. furcifera disturbed the feeding behavior of this pest to SRBSDV-infected rice, from preference to non-preference. Then, we further investigated the potential targets of M. anisopliae CQMa421 on the feeding behavior of S. furcifera after 0 h, 24 h and 48 h of infection by transcriptomic analysis via Illumina deep sequencing. A total of 93.27 GB of data was collected after sequencing, from which 91,125 unigenes were annotated, including 75 newly annotated genes. There were 1380 vs. 2187 and 137 vs. 106 upregulated and downregulated differentially expressed genes (DEGs) detected at 24 h and 48 h, respectively. The biological functions and associated metabolic processes of these genes were determined with the Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) databases. The results suggested that major of DEGs are involved in energy metabolism, biosynthesis, immune response, the FoxO signaling pathway, the MAPK signaling pathway and apoptosis in response to the fungal infection. Noteworthily, several olfactory-related genes, including odorant receptors and odorant binding proteins, were screened from these differentially expressed genes, which played critical roles in regulating the olfactory behavior of insects. Taken together, these results provide new insights for understanding the molecular mechanisms underlying fungus and host insect interaction, especially for olfactory behavior regulated by fungus.

show abstract

“…Several entomopathogenic fungal strains have been isolated from natural hosts and used for the control of some insect pests. For instance, two important fungal agents, Metarhizium anisopliae and Beauveria bassiana , showed good potential against insect pests such as Locusta migratoria [ 13 ], Alphitobius diaperinus [ 14 ], Chironomus riparius [ 15 ], and Helicoverpa armigera [ 16 ]. Additionally, a few specific M. anisopliae strains can be used against insecticide-resistant pests [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Analysis of Nilaparvata lugens microRNAs during Challenge with the Entomopathogenic Fungus Metarhizium anisopliae

Xie

Peng

Xia

2021

JoF

View full text Add to dashboard Cite

The resistance of the notorious rice pest Nilaparvata lugens to many insecticides has caused significant concerns. Our previous study demonstrated that the fungus Metarhizium anisopliae CQMa421 shows great potential for the control of this pest, but the interactions between them are still unclear. Thus, we further investigated fungal infection-related microRNAs (miRNAs) in N. lugens during M. anisopliae CQMa421 challenge using Illumina sequencing. In this study, we constructed twenty-four small RNA libraries over different time courses (i.e., 4 h, 8 h, 16 h, and 24 h). A total of 478.62 M clean reads were collected, with each sample producing more than 13.37 M reads, after the removal of low-quality reads. We identified 2324 miRNAs and their 11,076 target genes within the twenty-four libraries by bioinformatics analysis. Differentially expressed miRNAs (DEmiRNAs), including 58 (32 upregulated vs. 26 downregulated), 62 (30 upregulated vs. 32 downregulated), 126 (71 upregulated vs. 55 downregulated), and 109 (40 upregulated vs. 69 downregulated) DEmiRNAs were identified at 4 h, 8 h, 16 h, and 24 h post-infection, respectively. We further conducted Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis to predict the functions of all target genes of DEmiRNAs. These DEmiRNAs targets identified during 24 h of infection were primarily involved in energy metabolism, lysine degradation, the FoxO signaling pathway, ubiquitin-mediated proteolysis, the mRNA surveillance pathway, and the MAPK signaling pathway. Taken together, our results provide essential information for further study of the interactions between the entomopathogenic fungus M. anisopliae and N. lugens at the posttranscriptional level.

show abstract

Development of mycoinsecticide formulations with Beauveria bassiana and Metarhizium anisopliae for the control of lesser mealworm, Alphitobius diaperinus, in chicken broiler houses

Cited by 13 publications

References 44 publications

Transcriptomic Analysis Reveals the Impact of the Biopesticide Metarhizium anisopliae on the Immune System of Major Workers in Solenopsis invicta

Transcriptomic Analysis Reveals the Impact of the Biopesticide Metarhizium anisopliae on the Immune System of Major Workers in Solenopsis invicta

The Entomopathogenic Fungus Metarhizium anisopliae Affects Feeding Preference of Sogatella furcifera and Its Potential Targets’ Identification

Genome-Wide Identification and Analysis of Nilaparvata lugens microRNAs during Challenge with the Entomopathogenic Fungus Metarhizium anisopliae

Contact Info

Product

Resources

About