Development of Muscle Fibers in the Fetal Pig

Ashmore, C. R.; Addis, P. B.; Doerr, L.

doi:10.2527/jas1973.3661088x

Cited by 108 publications

(73 citation statements)

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“…In the bovine, the presence of the IGFII mRNA and both the IGFs receptors coincides with muscle differentiation [35]. This coincidence is found in other species such as in the ovine [2,34], in the porcine [3,25], and in the murine species [4,38]. Thus, in muscle cells, in vivo, IGF-11 might act as an autocrine pathway on muscle cell differentiation, as has been shown in vitro by Florini et al.…”

supporting

confidence: 55%

Characterisation and location of insulin-like-growth factor (IGF) receptors in the foetal bovine Semitendinosus muscle

Listrat¹,

Jammes²,

Djianne³

et al. 1999

Reprod. Nutr. Dev.

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supporting

confidence: 55%

Characterisation and location of insulin-like-growth factor (IGF) receptors in the foetal bovine Semitendinosus muscle

Listrat¹,

Jammes²,

Djianne³

et al. 1999

Reprod. Nutr. Dev.

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“…The muscle fibres are formed during foetal life, and in pigs the number is fixed before birth (Ashmore et al, 1973;Wigmore and Stickland, 1983;Christensen et al, 2000). As the number of muscle fibres in pigs is fixed before birth, postnatal muscle growth depends -E-mail: piam.nissen@agrsci.dk on growth of the individual muscle fibres.…”

Section: Introductionmentioning

confidence: 99%

In vitro primary satellite cell growth and differentiation within litters of pigs

Nissen

Oksbjerg

2009

Animal

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Postnatal muscle growth is dependent on satellite cell (SC) proliferation, differentiation and fusion to increase the DNA content of existing muscle fibres and thereby the capacity to synthesize protein. The purpose of the present study was to examine the ability of isolated SCs from low, medium and high weaning weight litter mates of pigs to proliferate and differentiate, and to affect protein synthesis and degradation after fusion into myotubes. At 6 weeks of age, SCs from the lowest weight (LW), medium weight (MW) and highest weight (HW) female pigs within eight litters were isolated. Thereby, eight cultures of SCs were established for each of the three weight groups within litter, representing three groups of SCs from pigs exhibiting differences in postnatal muscle growth performance. Proliferation was estimated as the number of viable cells at different time points after seeding. SC differentiation was evaluated by measuring the activity of the muscle-specific enzyme, creatine phosphokinase, and protein synthesis and degradation were measured by incorporation and release of 3 H-tyrosine, respectively. A tendency towards a difference in proliferation between SC cultures was found ( P 5 0.09). This was evident as the number of viable cells at day 3 was lower in cultures from LW pigs than from HW ( P , 0.05) and MW ( P , 0.01) pigs. Differentiation was significantly different between cultures ( P , 0.05). There was a significant difference between LW and MW cultures at 72 h ( P , 0.05), and a tendency towards a difference between LW and HW cultures at 45 h ( P 5 0.07). Protein synthesis per mg protein or per mg DNA did not differ among SC cultures from LW, MW and HW pigs. Neither did protein degradation rate differ significantly among SC cultures from LW, MW and HW pigs. Overall, the results show that SCs from LW pigs seem to proliferate and differentiate at a slower rate than SCs from MW and HW pigs. The results found in this study show no difference in the ability of SCs to affect protein synthesis or degradation between SCs from litter mates exhibiting different growth rates in vivo.

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“…Several studies have been carried out to describe the ontogenesis of this fiber type distribution (Davies, 1972;Thurley, 1972;Ashmore et al, 1973;Swatland and Cassens, 1973;Beermann et al, 1978;Wigmore and Stickland, 1983;Handel and Stickland, 1987). Pig myogenesis as in many mammalian species is a biphasic phenomenon with the sequential formation of two generations of muscle fibers.…”

Section: Introductionmentioning

confidence: 99%

Pattern of muscle fiber type formation in the pig

Lefaucheur¹,

Edom

Ecolan³

et al. 1995

Developmental Dynamics

139

120

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The aim of this study was to analyze the temporal sequence of expression of the myosin isoforms in the populations of muscle fibers in the pig and to bring more information on the origin of the strikingly different pattern of fiber composition and distribution between the deep medial red (oxido-glycolytic) and superficial white (glycolytic) portions of semitendinosus (ST) muscle. Muscle samples were taken from 49-, 55-, 75-, 90-, 103-, and 113-(birth) day-old fetuses, from 6-, 11-, 21-, 35-, 50-, and 80-day-old piglets, and from a 3-year-old pig. Our results confirm the sequential formation of primary and secondary generation fibers. The use of immunohistochemistry and heterologous monoclonal antibodies (mAb) directed against specific myosin heavy chain (MHC) isoforms revealed a different pattern of gene expression between the two portions of the ST muscle for both generations of fibers. By 75 days of gestation (dg), primary myotubes from the deep medial portion stained positively for the anti-slow MHC mAb and negatively for the adult anti-fast MHC, whereas the opposite was observed in the superficial portion. Secondary fibers never expressed slow MHC until late gestation. Instead, they expressed an adult fast MHC isoform as soon as they formed in the deep medial portion and later on in the superficial portion. From late gestation to the first 3 postnatal weeks, slow MHC began to be expressed in a subpopulation of secondary fibers. These fibers were in the direct vicinity of primary myotubes in the deep medial portion, whereas their location could not be established in the superficial portion. The remaining secondary fibers matured to type IIA in the direct vicinity of these type I fibers and to type IIB at the periphery of the islets. In both portions of the muscle, a subpopulation of secondary fibers, the first ones to express slow MHC, also transitorily expressed a MHC that was identical or closely related to the a-cardiac MHC during the early postnatal period. A third generation of small diameter fibers was observed shortly after birth and reacted with the anti-fetal MHC mAb; their destiny remains to be established. The present work reveals a remarkable pattern of MHC gene expression in the pig and raises many questions on the real nature of these isoforms. In order to answer these questions, we have undertaken to make a cDNA library of pig skeletal muscle and to screen 0 1995 WILEY-LISS, INC.this library with the same mAbs used in the present study. o 1995 Wiley-Liss, Inc.

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Development of Muscle Fibers in the Fetal Pig

Cited by 108 publications

References 0 publications

Characterisation and location of insulin-like-growth factor (IGF) receptors in the foetal bovine Semitendinosus muscle

Characterisation and location of insulin-like-growth factor (IGF) receptors in the foetal bovine Semitendinosus muscle

In vitro primary satellite cell growth and differentiation within litters of pigs

Pattern of muscle fiber type formation in the pig

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