Development of Multiplex Real-Time Polymerase Chain Reaction for Detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in Clinical Specimens

Hamzah, Zulhainan; Petmitr, Songsak; Mungthin, Mathirut; Leelayoova, Saovanee; Chavalitshewinkoon-Petmitr, Porntip

doi:10.4269/ajtmh.2010.10-0050

Cited by 43 publications

(34 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, since the recognition of the high prevalence of E moshkovskii in humans, 2 real-time PCRs have been developed to detect E histolytica, E dispar, and E moshkovskii in stool specimens. 30,31 There is currently no real-time PCR to detect E bangladeshi.…”

Section: Real-time Polymerase Chain Reactionmentioning

confidence: 99%

“…9 Since then, human isolates of E moshkovskii have been reported from at least 13 other countries, including both resource-poor and resource-rich countries: the United States, Italy, South Africa, India, Australia, Pakistan, Iran, Tanzania, Turkey, Malaysia, Thailand, Tunisia, and the United Arab Emirates. 9,[21][22][23][24][25][26][27][28][29][30][31][32][33] In most of these studies the ameba has not been associated with disease and was isolated from very few individuals. However, high prevalences of E moshkovskii were reported in some countries: 50% in Australia, 23 24.9% and 15.6% in 2 different study populations in India, 34,35 21.1% in Bangladesh, 9 13.2% in Tanzania, 26 13.2% in Pakistan, 24 and 5.2% in Iran.…”

Section: Entamoeba Disparmentioning

confidence: 99%

See 1 more Smart Citation

Intestinal Amebae

Ali¹

2015

Clinics in Laboratory Medicine

View full text Add to dashboard Cite

Among the Entamoeba species that infect humans, Entamoeba histolytica causes diseases, Entamoeba dispar is a harmless commensal, Entamoeba moshkovskii seems to be a pathogen, and the pathogenicity of Entamoeba bangladeshi remains to be investigated. Species-specific detection needed for treatment decisions and for understanding the epidemiology and pathogenicity of these amebae. Antigen-based detection methods are needed for E dispar, E moshkovskii, and E bangladeshi; and molecular diagnostic test capable of detecting E histolytica, E dispar, E moshkovskii, and E bangladeshi simultaneously in clinical samples. Next-generation sequencing of DNA from stool is needed to identify novel species of Entamoeba.

show abstract

Section: Real-time Polymerase Chain Reactionmentioning

confidence: 99%

Section: Entamoeba Disparmentioning

confidence: 99%

Intestinal Amebae

Ali¹

2015

Clinics in Laboratory Medicine

View full text Add to dashboard Cite

show abstract

“…We selected the 18s ribosomal gene for RPA because of its high copy number and because PCR studies have demonstrated its usefulness to distinguish Entamoeba species. [15][16][17] For the RPA reactions, we used a set of primers of 25 nt in size compatible with RPA assays that amplify specifically a 132 amplicon of E. histolytica but not E. dispar. We adapted the RPA for LF detection by using an internal duallabeled probe that allowed the specific detection of the amplicon on LF paper strips.…”

Section: Discussionmentioning

confidence: 99%

Detection of Entamoeba histolytica by Recombinase Polymerase Amplification

Nair¹,

Rebolledo²,

White³

et al. 2015

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Amebiasis is an important cause of diarrheal disease worldwide and has been associated with childhood malnutrition. Traditional microscopy approaches are neither sensitive nor specific for Entamoeba histolytica. Antigen assays are more specific, but many cases are missed unless tested by molecular methods. Although polymerase chain reaction (PCR) is effective, the need for sophisticated, expensive equipment, infrastructure, and trained personnel limits its usefulness, especially in the resource-limited, endemic areas. Here, we report development of a recombinase polymerase amplification (RPA) method to detect E. histolytica specifically. Using visual detection by lateral flow (LF), the test was highly sensitive and specific and could be performed without additional equipment. The availability of this inexpensive, sensitive, and field-applicable diagnostic test could facilitate rapid diagnosis and treatment of amebiasis in endemic regions.

show abstract

“…If there were sequential serologic data for E. histolytica infections, the method described in the current study could be used to estimate the prevalence. Because E. histolytica is morphologically indistinguishable from E. dispar, [28][29][30][31] an ELISA was used to investigate the seroprevalence of E. histolytica infections.…”

Section: Discussionmentioning

confidence: 99%

Seroprevalence of Entamoeba histolytica Infection in China

Yang¹,

Chen²,

Wu³

et al. 2012

The American Society of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. The seroprevalence of Entamoeba histolytica infection in the residents of seven provinces in China was examined by using an enzyme-linked immunosorbent assay with a crude antigen and a recombinant surface antigen, C-Igl, of the parasites. A total of 1,312 serum samples were investigated. The positivity rates for these two antigens were 11.05% and 6.25%, respectively. There was no significant difference in the seropositivity to E. histolytica between men and women. We used a logistic regression model and maximal-likelihood methods to estimate the prevalence of E. histolytica infection from sequential serologic data. Seropositivity in Sichuan, Guizhou, and Sinkiang Provinces was higher than that in Beijing, Shanghai, and Qinghai Provinces. The present study provides an overview of seropositivity to E. histolytica infection in seven provinces in China and use the logistic regression model estimation method to achieve a more accurate measure of amebiasis prevalence.

show abstract

Development of Multiplex Real-Time Polymerase Chain Reaction for Detection of Entamoeba histolytica, Entamoeba dispar, and Entamoeba moshkovskii in Clinical Specimens

Cited by 43 publications

References 24 publications

Intestinal Amebae

Intestinal Amebae

Detection of Entamoeba histolytica by Recombinase Polymerase Amplification

Seroprevalence of Entamoeba histolytica Infection in China

Contact Info

Product

Resources

About