Development of Multiplex PCR-Ligase Detection Reaction Assay for Detection of West Nile Virus

Rondini, Simona; Pingle, Maneesh; Das, Sanchita; Tesh, Robert B.; Rundell, Mark S.; Hom, J.; Stramer, Susan L.; Turner, Kathleen; Rossmann, Susan N.; Lanciotti, Robert S.; Spier, E. G.; Muñoz‐Jordán, Jorge L.; Larone, Davise H.; Spitzer, Eric D.; Barany, Francis; Golightly, Linnie M.

doi:10.1128/jcm.02335-07

Cited by 32 publications

(33 citation statements)

References 34 publications

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“…Any low-level polymerase errors remain unselected; thus, background noise is minimized. This method has been used in the detection of some viruses, oncogenes, and tumor-suppressor genes (Khanna et al, 1999;Rondini et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Analysis of the genetic effects of CAPN1 gene polymorphisms on chicken meat tenderness

JingTing¹,

Zhang²,

Shan³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. The micromolar calcium-activated neutral protease gene (CAPN1) is a physiological candidate gene for meat tenderness. Four previously identified single nucleotide polymorphism (SNP) markers located within the CAPN1 gene were evaluated for their associations with variation in the meat tenderness of a Chinese indigenous chicken breed, a higher meat quality breed (i.e., Qingyuan partridge chicken), and the commercial Recessive White chicken breed. WarnerBratzler shear force measurements were used to determine tenderness phenotypes for all animals; intramuscular fat (IMF) content and rate of water loss in the breast muscles were also measured. Genotyping was performed by the polymerase chain reaction-ligase detection reaction method. Polymorphisms were identified for all markers, except CAPN1 2546. The frequency of allele T was zero, and allele C was fixed for CAPN1 2546 in the studied populations. The SNP CAPN1 3535 in the CAPN1 gene was significantly associated with tenderness and other meat quality traits, where animals inheriting the AA genotype had smaller shear force values, lower water loss rates, and higher IMF contents. Moreover, H1 (AAA) was the most advantageous haplotype for meat tenderness. The results of this study confirm some previously documented associations. Furthermore, novel associations have been 1393-1403 (2015) identified that, following validation in other populations, could be incorporated into breeding programs to improve meat quality.

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Section: Discussionmentioning

confidence: 99%

Analysis of the genetic effects of CAPN1 gene polymorphisms on chicken meat tenderness

JingTing¹,

Zhang²,

Shan³

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…Any low-level polymerase errors remain unselected, and thus contribute only minimal background noise. It has been used in the detection of some viruses, oncogenes and tumor-suppressor genes (Khanna et al, 1999;Rondini et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional co-activator PGC-1α gene is associated with chicken skeletal muscle fiber types

JingTing¹,

Xu²,

Zhang³

et al. 2014

Genet. Mol. Res.

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ABSTRACT. The peroxisome proliferators-activated receptor-γ coactivator-1α (PGC-1α) is a candidate gene for meat quality traits because of its prominent role in muscle fiber type switching and determination. We investigated the effects of the PGC-1α gene on chicken skeletal muscle fiber type switching and on other meat quality traits. Single nucleotide polymorphisms were detected by PCR-SSCP and DNA sequencing, and then genotyping was performed by PCR-ligation detection reaction methods. Skeletal muscle fiber types, intramuscular fat content, shear forces, and water loss rate of the gastrocnemius lateralis muscle were measured in Qingyuan Partridge chickens and Recessive White chickens. Four SNPs, C171T in exon2, C384T in exon3, G646A in exon5, and A948G in exon8 were detected. Marker-trait association analysis indicated that G646A polymorphism was associated with skeletal myofiber type and that H1 (CCAA) was the most advantageous haplotype for skeletal myofiber type. We concluded that polymorphisms of the PGC-1α gene and their haplotypes are associated with chicken skeletal myofiber type traits.

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“…Any low-level polymerase errors remain unselected, and so only contribute minimal background noise. PCR-LDR has been used in the detection of some viruses, oncogenes, and tumor-suppressor genes (Khanna et al, 1999;Rondini et al, 2008). The results showed that all of the egg production traits were significantly different between RW chickens and QY chickens, indicating that these 2 breeds might have different genetic backgrounds.…”

Section: Discussionmentioning

confidence: 99%

Association of chicken growth hormone polymorphisms with egg production

Su¹,

JingTing²,

Zhang³

et al. 2014

Genet. Mol. Res.

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Development of Multiplex PCR-Ligase Detection Reaction Assay for Detection of West Nile Virus

Cited by 32 publications

References 34 publications

Analysis of the genetic effects of CAPN1 gene polymorphisms on chicken meat tenderness

Analysis of the genetic effects of CAPN1 gene polymorphisms on chicken meat tenderness

Transcriptional co-activator PGC-1α gene is associated with chicken skeletal muscle fiber types

Association of chicken growth hormone polymorphisms with egg production

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