Development of multiple reaction monitoring (MRM) assays to identify <i>Brucella abortus</i> proteins in the serum of humans and livestock

Husain, Aliabbas A.; Pinto, Sneha M.; Subbannayya, Yashwanth; Kapoor, Saketh; Khulkhule, Payal R; Bhartiya, Nidhi M; Prasad, T. S. Keshava; Daginawala, Hatim F.; Singh, Lakhan; Kashyap, Rajpal S.

doi:10.1002/prca.202200009

Cited by 2 publications

(1 citation statement)

References 16 publications

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“…Transitions for four peptides were found in various clinically confirmed and suspected human and cattle serum samples using high-throughput MRM tests. Of them, peptide NAIYDVVTR with 100% specificity was repeatedly found in the clinically proved serum samples of both people and livestock, and it corresponds to the B. abortus protein (BruAb2_0537) ( 216 ).…”

Section: Immunitymentioning

confidence: 99%

Brucellae as resilient intracellular pathogens: epidemiology, host–pathogen interaction, recent genomics and proteomics approaches, and future perspectives

Dawood,

Elrashedy,

Nayel

et al. 2023

Front. Vet. Sci.

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Brucellosis is considered one of the most hazardous zoonotic diseases all over the world. It causes formidable economic losses in developed and developing countries. Despite the significant attempts to get rid of Brucella pathogens in many parts of the world, the disease continues to spread widely. Recently, many attempts proved to be effective for the prevention and control of highly contagious bovine brucellosis, which could be followed by others to achieve a prosperous future without rampant Brucella pathogens. In this study, the updated view for worldwide Brucella distribution, possible predisposing factors for emerging Brucella pathogens, immune response and different types of Brucella vaccines, genomics and proteomics approaches incorporated recently in the field of brucellosis, and future perspectives for prevention and control of bovine brucellosis have been discussed comprehensively. So, the current study will be used as a guide for researchers in planning their future work, which will pave the way for a new world without these highly contagious pathogens that have been infecting and threatening the health of humans and terrestrial animals.

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Section: Immunitymentioning

confidence: 99%

Brucellae as resilient intracellular pathogens: epidemiology, host–pathogen interaction, recent genomics and proteomics approaches, and future perspectives

Dawood,

Elrashedy,

Nayel

et al. 2023

Front. Vet. Sci.

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Deciphering the structure, function, and mechanism of lysine acetyltransferase cGNAT2 in cyanobacteria

Jia,

Yang,

Liu

et al. 2023

Plant Physiology

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Lysine acetylation is a conserved regulatory post-translational protein modification that is performed by lysine acetyltransferases (KATs). By catalyzing the transfer of acetyl groups to substrate proteins, KATs play critical regulatory roles in all domains of life; however, no KATs have yet been identified in cyanobacteria. Here, we tested all predicted KATs in the cyanobacterium Synechococcus sp. PCC 7002 (Syn7002) and demonstrated that A1596, which we named cyanobacterial Gcn5-related N-acetyltransferase (cGNAT2), can catalyze lysine acetylation in vivo and in vitro. Eight amino acid residues were identified as the key residues in the putative active site of cGNAT2, as indicated by structural simulation and site-directed mutagenesis. The loss of cGNAT2 altered both growth and photosynthetic electron transport in Syn7002. In addition, quantitative analysis of the lysine acetylome identified 548 endogenous substrates of cGNAT2 in Syn7002. We further demonstrated that cGNAT2 can acetylate NAD(P)H dehydrogenase J (NdhJ) in vivo and in vitro, with the inability to acetylate K89 residues, thus decreasing NdhJ activity and affecting both growth and electron transport in Syn7002. In summary, this study identified a KAT in cyanobacteria and revealed that cGNAT2 regulates growth and photosynthesis in Syn7002 through an acetylation-mediated mechanism.

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Development of multiple reaction monitoring (MRM) assays to identify Brucella abortus proteins in the serum of humans and livestock

Cited by 2 publications

References 16 publications

Brucellae as resilient intracellular pathogens: epidemiology, host–pathogen interaction, recent genomics and proteomics approaches, and future perspectives

Brucellae as resilient intracellular pathogens: epidemiology, host–pathogen interaction, recent genomics and proteomics approaches, and future perspectives

Deciphering the structure, function, and mechanism of lysine acetyltransferase cGNAT2 in cyanobacteria

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