Development of multiple inhibitor tolerant yeast via adaptive laboratory evolution for sustainable bioethanol production

Hemansi,; Himanshu, .; Patel, Anil Kumar; Saini, Jitendra Kumar; Singhania, Reeta Rani

doi:10.1016/j.biortech.2021.126247

Cited by 38 publications

(17 citation statements)

References 48 publications

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“…Figure 3 shows the SSF strategy for ethanol production. High inhibitor concentration due to high solid loading may be tackled by using multiple inhibitor tolerant yeast which may be developed by adaptive evolution [9]. It has been observed that batch fermentation results in lower ethanol concentration in broth and usually reaches ~4% ethanol concentration in the broth which is also not feasible economically for distillation to obtain anhydrous ethanol [12,94].…”

Section: Simultaneous Saccharification and Fermentation Of Biomassmentioning

confidence: 99%

“…Chemically it consists of majorly three components namely; cellulose, hemicellulose and lignin. Holocellulose (cellulose + hemicellulose) part can be hydrolyzed by acids or by enzymes into fermentable sugars which can be further converted into ethanol via yeasts such as Saccharomyces cerevisiae or Kluveromyces [9]. To deal with biomass containing lignin is challenging especially when our target is the holocellulosic fraction.…”

Section: Introductionmentioning

confidence: 99%

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Advances and Challenges in Biocatalysts Application for High Solid-Loading of Biomass for 2nd Generation Bio-Ethanol Production

et al. 2022

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Growth in population and thereby increased industrialization to meet its requirement, has elevated significantly the demand for energy resources. Depletion of fossil fuel and environmental sustainability issues encouraged the exploration of alternative renewable eco-friendly fuel resources. Among major alternative fuels, bio-ethanol produced from lignocellulosic biomass is the most popular one. Lignocellulosic biomass is the most abundant renewable resource which is ubiquitous on our planet. All the plant biomass is lignocellulosic which is composed of cellulose, hemicellulose and lignin, intricately linked to each other. Filamentous fungi are known to secrete a plethora of biomass hydrolyzing enzymes. Mostly these enzymes are inducible, hence the fungi secrete them economically which causes challenges in their hyperproduction. Biomass’s complicated structure also throws challenges for which pre-treatments of biomass are necessary to make the biomass amorphous to be accessible for the enzymes to act on it. The enzymatic hydrolysis of biomass is the most sustainable way for fermentable sugar generation to convert into ethanol. To have sufficient ethanol concentration in the broth for efficient distillation, high solid loading ~<20% of biomass is desirable and is the crux of the whole technology. High solid loading offers several benefits including a high concentration of sugars in broth, low equipment sizing, saving cost on infrastructure, etc. Along with the benefits, several challenges also emerged simultaneously, like issues of mass transfer, low reaction rate due to water constrains in, high inhibitor concentration, non-productive binding of enzyme lignin, etc. This article will give an insight into the challenges for cellulase action on cellulosic biomass at a high solid loading of biomass and its probable solutions.

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Section: Simultaneous Saccharification and Fermentation Of Biomassmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Advances and Challenges in Biocatalysts Application for High Solid-Loading of Biomass for 2nd Generation Bio-Ethanol Production

et al. 2022

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“…12 It is a powerful strategy to acquire desired phenotype of inhibitor tolerance in the fermenting microorganism by cultivating it under progressively increasing levels of inhibitors, followed by screening of the tolerant strains. 13 Furthermore, ALE provides valuable insights into the genotype−phenotype relationship by investigating a time series of genomic changes in evolved strains and uncovering the tolerance mechanisms of strains under certain specific stress conditions. 14 Ideally, it would obtain robust strains with desired phenotypes through nongenetic modifications, making them suitable for food processing and probiotic utilization.…”

Section: Introductionmentioning

confidence: 99%

“…By subjecting microorganisms to targeted environmental pressures and employing long-term domestication techniques, it is able to obtain evolved strains with specific phenotypes . It is a powerful strategy to acquire desired phenotype of inhibitor tolerance in the fermenting microorganism by cultivating it under progressively increasing levels of inhibitors, followed by screening of the tolerant strains . Furthermore, ALE provides valuable insights into the genotype–phenotype relationship by investigating a time series of genomic changes in evolved strains and uncovering the tolerance mechanisms of strains under certain specific stress conditions .…”

Section: Introductionmentioning

confidence: 99%

Unraveling the Genetic Adaptations in Cell Surface Composition and Transporters of Lactiplantibacillus plantarum for Enhanced Acid Tolerance

Zhu,

Sun,

Zhang

et al. 2024

J. Agric. Food Chem.

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This study employed adaptive laboratory evolution to improve the acid tolerance of Lactiplantibacillus plantarum, a vital strain in food fermentation and a potential probiotic. Phenotype and genomic analyses identified the overexpression of stress response proteins, ATP synthases, and transporters as pivotal in conferring acid tolerance to the evolved strains. These adaptations led to a shorter lag phase, improved survival rates, and higher intracellular pH values compared to the wild-type strain under acid stress conditions. Additionally, the evolved strains showed an increased expression of genes in the fatty acid synthesis pathway, resulting in a higher production of unsaturated fatty acids. The changes in cell membrane composition possibly prevented H + influx, while mutant genes related to cell surface structure contributed to observed elongated cells and thicker cell surface. These alterations in cell wall and membrane composition, along with improved transporter efficiency, were key factors contributing to the enhanced acid tolerance in the evolved strains.

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“…Similarly, Dong et al enhanced the substrate tolerance of Pseudomonas putida using ARTP mutagenesis and obtained a high-yield mutant strain with a 42% increase in nicotinic acid yield . Adaptive laboratory evolution (ALE) which could simulate the natural evolution of microorganisms under environmental stress and finally obtain the desired phenotype is another powerful method of microbial mutation breeding . An evolved E.…”

Section: Introductionmentioning

confidence: 99%

Adaptive Evolution and Metabolic Engineering Boost Lycopene Production in Saccharomyces cerevisiae via Enhanced Precursors Supply and Utilization

Zhou

Liang

et al. 2023

J. Agric. Food Chem.

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Lycopene is a red carotenoid with remarkable antioxidant activity, which has been widely used in food, cosmetics, medicine, and other industries. Production of lycopene in Saccharomyces cerevisiae provides an economic and sustainable means. Many efforts have been done in recent years, but the titer of lycopene seems to reach a ceiling. Enhancing the supply and utilization of farnesyl diphosphate (FPP) is generally regarded as an efficient strategy for terpenoid production. Herein, an integrated strategy by means of atmospheric and room-temperature plasma (ARTP) mutagenesis combined with H2O2-induced adaptive laboratory evolution (ALE) was proposed to improve the supply of upstream metabolic flux toward FPP. Enhancing the expression of CrtE and introducing an engineered CrtI mutant (Y160F&N576S) increased the utilization of FPP toward lycopene. Consequently, the titer of lycopene in the strain harboring the Ura3 marker was increased by 60% to 703 mg/L (89.3 mg/g DCW) at the shake-flask level. Eventually, the highest reported titer of 8.15 g/L of lycopene in S. cerevisiae was achieved in a 7 L bioreactor. The study highlights an effective strategy that the synergistic complementarity of metabolic engineering and adaptive evolution facilitates natural product synthesis.

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Development of multiple inhibitor tolerant yeast via adaptive laboratory evolution for sustainable bioethanol production

Cited by 38 publications

References 48 publications

Advances and Challenges in Biocatalysts Application for High Solid-Loading of Biomass for 2nd Generation Bio-Ethanol Production

Advances and Challenges in Biocatalysts Application for High Solid-Loading of Biomass for 2nd Generation Bio-Ethanol Production

Unraveling the Genetic Adaptations in Cell Surface Composition and Transporters of Lactiplantibacillus plantarum for Enhanced Acid Tolerance

Adaptive Evolution and Metabolic Engineering Boost Lycopene Production in Saccharomyces cerevisiae via Enhanced Precursors Supply and Utilization

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