Development of morphological heterogeneity in glucose-limited chemostat cultures of Aspergillus oryzae

Effect of branch frequency inAspergillus oryzae on protein secretion and culture viscosity

“…Differences in the length of time mycelia were subject to shear stresses, differences in specific growth rates and the effects of autolysis (in fed-batch culture) are thought to account for some of the differences observed in G between different culture systems (Smith et al, 1990;van Suijdam and Metz, 1981;Prosser, 1995). Withers et al (1994) have also observed that G values may differ considerably depending on the culture system employed.…”

The Mycelium as an Integrated Entity

“…Until recently, this hypothesis was supported by the observation that, although mutants more highly branched (shorter G values) than their parental strains have frequently been isolated, mutants more sparsely branched (longer G values) than their parental strains have never been reported. However, recently, Withers et al (1994) and M.G. Wiebe (unpubl.…”

Circadian rhythmicity during prolonged chemostat cultivation of Neurospora crassa

“…These studies have used bacteria or budding yeast which are considerably easier to culture continuously than Wlamentous fungi that may switch growth forms depending on the culture conditions, forming pellets or clumps of tissue that result in a non homogenous culture. Although chemostat cultures of a number of Wlamentous fungi have been reported including Penicillium chrysogenum (Withers et al, 1995), Fusarium venenatum (Wiebe et al, 1992(Wiebe et al, , 1996, Trichoderma reesei (Pakula et al, 2005), Aspergillus niger (Mainwaring et al, 1999;Swift et al, 1998Swift et al, , 2000, Aspergillus nidulans (Rowley and Pirt, 1972;Sims et al, 2005) and Aspergillus oryzae (Withers et al, 1994), this is the Wrst report of conditions for successful growth of N. crassa in chemostat culture. Our results indicate that under these conditions the circadian clock runs for a period of at least six days in the dark after setting the clock through exposure to light.…”