Development of monoclonal antibody and lateral test strip for sensitive detection of clenbuterol and related β<sub>2</sub>-agonists in urine samples

A dual-labelled immunoassay using goldmag nanoparticles (GMNPs) and CdTe quantum dots (QDs) was applied for the quantification of casein in milk. In this method, anti-casein monoclonal antibody bound to GMNPs was used as capture probe, and the anti-casein polyclonal antibody, labelled with CdTe QDs, was employed as detection probe. This dual-labelled immunoassay was optimized and applied in the testing of casein content of three brands of commercial milks. The results showed a good linear tendency between casein concentrations and fluorescent intensities in the range of 4.617-289.9 ng/mL, and the half inhibition concentration (IC 50 ) was 36.59 ng/mL. Besides, the recovery rates of this developed immunoassay were in accordance with those in traditional enzyme-linked immunosorbent assay for rapid detection of casein content in commercial milks. It suggested that this dual-labelled immunoassay was reliable, and could provide important theoretical value and practical significance in the identification and quantification of milk allergens. ARTICLE HISTORY

Section: Introductionmentioning

confidence: 99%

Dual-labelled immunoassay with goldmag nanoparticles and quantum dots for quantification of casein in milk

Wang

et al. 2017

“…Ic-ELISA was developed as previously described (Khaemba et al, 2016;Suryoprabowo, Liu, Peng, Kuang, & Xu, 2014). Briefly, coating antigen was dissolved in CB (0.05 M, pH 9.6), added to each well of 96-well plates (100 µl), and incubated at 37°C for 2 h. After washing the plates, each well was blocked with 200 µl blocking buffer (2% gelatin in 0.05 M CB, pH 9.6) and incubated for 2 h at 37°C.…”

Section: Development Of Ic-elisamentioning

confidence: 99%

Development of ic-ELISA and lateral-flow immunochromatographic assay strip for the detection of folic acid in energy drinks and milk samples

Kong

Liu

Song

et al. 2016

Self Cite

Folic acid (FA) is an important vitamin for human growth and development, especially for pregnant women. A sensitive, rapid, and accurate FA detection method is required to assess the nutritional quality and safety of foods. A monoclonal antibody against FA was prepared for the development of indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and lateral-flow immunochromatographic assay (ICA) strip. The 50% inhibitory concentration and limit of detection of ic-ELISA were 0.12 and 0.018 ng/ml, respectively. The visual limit of detection and cut-off values of the lateral-flow ICA strip were 0.5 and 2.5 ng/ ml, respectively. Using the ICA strip, FA recovery rates were 89-98% from energy drinks and 73-87% for milk samples and were in good agreement with those obtained from the conventional microbiological assay method. Our developed methods are sensitive, convenient, effective, and suitable for on-site detection and rapid mass screening of food samples. ARTICLE HISTORY

“…The colloidal gold nano particles (GNPs) were prepared according to the method described in (Chen et al, 2015;Khaemba et al, 2015). A volume of 2 ml of freshly prepared 1% (w/v) trisodium citrate was added to 100 ml of HAuCl 4 ·4H 2 O solution (0.01%, w/v) (previously heated up to its boiling point under continuous stirring).…”

Section: Synthesis Of Gold Nanoparticlesmentioning

confidence: 99%

Development of an immunochromatographic assay for hexestrol and diethylstilbestrol residues in milk

Mukunzi

Tochi

Isanga

et al. 2016

Self Cite

Hexestrol (HES) and Diethylstilbestrol (DES) are synthetic hormones, which have been found abuse use in animal-origin food production. We developed an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immune-chromatographic strip to detect these compounds based on monoclonal antibody. Under optimized conditions, the half-inhibition concentration (IC 50 ) values of ic-ELISA were 0.15 µgL −1 and 0.23 µgL −1 for HES and DES, respectively. Spiked milk samples were analyzed. The recovery of both synthetic hormones in the milk samples was 60.48-102.19% (HES) and 89.34-100.16% (DES). In immunechromatographic assay, the visual cutoff values at 0.5 and 1.0 µgL −1 respectively for HES and DES, which allow us to detect milk samples of a concentration low to 10 µgL −1 for HES and 15 µgL −1 for DES. ARTICLE HISTORY