Development of Monoclonal Antibodies against HIV-1 p24 Protein and Its Application in Colloidal Gold Immunochromatographic Assay for HIV-1 Detection

Abstract

Human immunodeficiency virus type 1 (HIV-1) p24 protein is the most abundant viral protein of HIV-1. This protein is secreted in blood serum at high levels during the early stages of HIV-1 infection, making it a biomarker for early diagnosis. In this study, a colloidal gold immunochromatographic assay (GICA) was established for detecting p24 protein using mouse monoclonal antibodies (mAbs). The HIV-1 p24 protein was expressed in E. coli strain BL21 and the purified protein was used to immunize mice. Stable hybridoma cell lines secreting anti-p24 monoclonal antibodies were obtained after ELISA screening and subcloning by limiting dilution. 34 different capture and labeling mAb pairs were selected by a novel antibody-capture indirect sandwich ELISA and then applied in GICA to detect p24 protein. The GICA method has a limit of detection (LOD) of 25 pg/mL and could detect p24 protein in all 10 positive samples obtained from the National Reference of HIV-1 p24 antigen. Out of 153 negative samples tested, 3 false positives results were obtained. The overall specificity of this test was 98.03%. The good sensitivity and specificity of this method make it a suitable alternative to provide a more convenient and efficient tool for early diagnosis of HIV infection.

Consistent condom use among men who pay for sex in sub-Saharan Africa: Empirical evidence from Demographic and Health Surveys

“…Globally, research on condom use among men has mainly been conducted in Asian countries such as Cambodia [ 19 ], China [ 20 , 21 ], India [ 22 – 24 ], Singapore [ 25 ], Indonesia [ 26 ], with a few focusing on Middle East [ 1 ] and Africa [ 27 , 28 ]. Generally, such studies have revealed the predictors of consistent condom use among homosexual males [ 19 , 27 , 29 ], young dating adults [ 30 ], and men who patronize paid sex [ 22 ].…”

Screening of Non-catalytic Integrase Inhibitors for Anti-HIV Drug Development

“…The capsid protein, p24, is the most abundant protein of HIV [22,23] and commonly used in early detection and diagnosis of AIDS/HIV. [23][24][25][26] Given the low anti-HIV e cacy of BI22436 in our TZM-bl system, we developed a novel p24 ELISA assay to validate its antiviral activity and compared the results (Fig.…”

Study on Suitable Analysis Method for HIV-1 Non-catalytic Integrase Inhibitor

“…The capsid protein, p24, is the most abundant protein of HIV [22,23] and commonly used in early detection and diagnosis of AIDS/HIV. [23][24][25][26] Given the low anti-HIV e cacy of BI22436 in our TZM-bl system, we developed a novel p24 ELISA assay to validate its antiviral activity and compared the results (Fig 2).…”