Development of molecular and antigenic-based rapid tests for the identification of African swine fever virus in different tissues

A semi-quantitative visual lateral flow immunoassay for SARS-CoV-2 antibody detection for the follow-up of immune response to vaccination or recovery

“…The test lines were located as close as possible to the sample well to minimize the contact time between the target antibodies and the detector before they reached the capture reagent. Actually, when the same reagent was used for capturing and detection, a competition for the binding to target antibodies occurred, analogously to what happens for the single-epitope sandwich in the antigenic LFIA 75,83,84 . The effect of the competition was a reduction of the sensitivity, which could be counteracted efficiently by the precaution of narrowing the distance between the test lines and the sample application point.…”

“…The effect of the competition was a reduction of the sensitivity, which could be counteracted efficiently by the precaution of narrowing the distance between the test lines and the sample application point. 75,83,84…”

“…The effect of the competition was a reduction of the sensitivity, which could be counteracted efficiently by the precaution of narrowing the distance between the test lines and the sample application point. 75,83,84 The concentration of the second test line (T2) was defined according to the ability of correctly classifying four samples chosen to have a high titre (#1001, 5680 AU mL À1 ), a mediumhigh titre (#1010, 3238 AU mL À1 ), a medium-high titre (#1048, 417 AU mL À1 ), and a low titre (#1050, 90.5 AU mL À1 ). In particular, the interpretation rule was defined as follows: the high-titre sample should provide two coloured test lines, the lowtitre sample should provide one coloured test line, and the negative control should provide no coloured test lines.…”

Label-Free Detection of African Swine Fever and Classical Swine Fever in the Point-of-Care Setting Using Photonic Integrated Circuits Integrated in a Microfluidic Device

“…One such attempt resulted in the development of a rapid lateral flow assay with 99.8% specificity and 84.52% sensitivity for ASF detection [45]. Another study presented a lateral flow assay with 59.1% sensitivity (95% CI, 49.1-69.1) and 91.7% specificity (95% CI, 84.7-98.7) when tested with tissue samples [46]. In the nucleic acidbased category, a study evaluated the TripleE system for nucleic acid extraction in field conditions followed by PCR testing using thermal cyclers suitable for field operations [47].…”