Development of microwave-assisted extraction and liquid chromatography-tandem mass spectrometry for determination of maleic hydrazide residues in tobacco

Wang, Lei-jun; Fei, Ting; Qi, Dawei; Sha, Yunfei; Da, Wu; Liu, Baizhan

doi:10.1039/c5ay00187k

Cited by 4 publications

(8 citation statements)

References 24 publications

(18 reference statements)

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“…Since MH presents as free MH, glucose‐MH conjugates, and lignin bound MH in tobacco, aggressive extraction conditions are required to fully extract MH. Both caustic and acidic conditions at elevated temperatures have shown to work well and also have the added benefit of hydrolyzing the glucoside conjugates in the process. However, in the reported acidic extraction procedures, various extraction approaches were employed, including heating reflux, sonication, and microwave assisted extraction.…”

Section: Resultsmentioning

confidence: 99%

“…The LC separation was performed according to a published method with some modifications. A hypercarb porous graphitic carbon LC column (150 × 4.6 mm, 5 µm) was used, and the column temperature was kept at 40°C.…”

Section: Methodsmentioning

confidence: 99%

“…Various techniques have been established for the determination of MH, including colorimetric methods, chromatographic methods, electrochemical methods, etc. Among them, as the powerful separation technique, chromatographic methods are the most widely employed approaches for MH analysis in different matrices such as garlic, potato, and tobacco.…”

Section: Introductionmentioning

confidence: 99%

“…In HPLC based methods, MH residues were usually extracted with methanol or HCl by multiple partition steps, and finally analyzed using HPLC with UV detector at wavelengths ranged from 303 to 330 nm. In recent years, with the advancement of instrumentation, LC–MS/MS based methods have been established for determination of MH only or MH and other pesticides simultaneously …”

Section: Introductionmentioning

confidence: 99%

“…Renaud et al., Yang, and Wang et al. reported the determination of free and bound MH residues in tobacco with HCl hydrolysis by GC–NPD, HPLC–UV, and LC–MS/MS, respectively. Dias and Duncan investigated free and bound MH in potato tubers with HCl and β‐D‐glucosidase treatment .…”

Section: Introductionmentioning

confidence: 99%

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Use of autoclave extraction and liquid chromatography with tandem mass spectrometry for determination of maleic hydrazide residues in tobacco

Deng

Bian

Yang

et al. 2019

J of Separation Science

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Maleic hydrazide has been extensively used as an effective growth regulator in tobacco sucker control. After application, maleic hydrazide distributes itself throughout the tobacco plant where it can exist as free, or forms glucoside conjugates with glucose, or becomes bound with lignin. Among them, free maleic hydrazide and its glucoside conjugates are extractable under conventional solvent extraction, while lignin bound maleic hydrazide is claimed to be non-extractable. Herein, an autoclave extraction method has been developed to extract maleic hydrazide effectively, in which tobacco samples are extracted in an autoclave at 130 • C for 1 h using 4 M hydrochloric acid.Under such pressurized hot acidic water conditions, lignin bound maleic hydrazide can be released. Meanwhile, glucoside conjugates are hydrolyzed. Total maleic hydrazide is detected by liquid chromatography coupled with tandem mass spectrometry, and the quantitative results coincide well with that obtained from the international standard method. The proposed autoclave extraction with liquid chromatography and tandem mass spectrometry method exhibits excellent linearity in the range of 5-200 mg/kg (R 2 = 0.9998), the matrix matched limit of detection and limit of quantification is 0.68 and 2.27 mg/kg, respectively. This method is simple and improves sample capacity, providing an effective approach to monitoring maleic hydrazide residues in tobacco. K E Y W O R D Sautoclave extraction, liquid chromatography, maleic hydrazide, tandem mass spectrometry, tobacco 2390

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Use of autoclave extraction and liquid chromatography with tandem mass spectrometry for determination of maleic hydrazide residues in tobacco

Deng

Bian

Yang

et al. 2019

J of Separation Science

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Study of different HILIC, mixed-mode, and other aqueous normal-phase approaches for the liquid chromatography/mass spectrometry-based determination of challenging polar pesticides

et al. 2016

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The aim of the study was to evaluate the performance of different chromatographic approaches for the liquid chromatography/mass spectrometry (LC-MS(/MS)) determination of 24 highly polar pesticides. The studied compounds, which are in most cases unsuitable for conventional LC-MS(/MS) multiresidue methods were tested with nine different chromatographic conditions, including two different hydrophilic interaction liquid chromatography (HILIC) columns, two zwitterionic-type mixed-mode columns, three normal-phase columns operated in HILIC-mode (bare silica and two silica-based chemically bonded columns (cyano and amino)), and two standard reversed-phase C18 columns. Different sets of chromatographic parameters in positive (for 17 analytes) and negative ionization modes (for nine analytes) were examined. In order to compare the different approaches, a semi-quantitative classification was proposed, calculated as the percentage of an empirical performance value, which consisted of three main features: (i) capacity factor (k) to characterize analyte separation from the void, (ii) relative response factor, and (iii) peak shape based on analytes' peak width. While no single method was able to provide appropriate detection of all the 24 studied species in a single run, the best suited approach for the compounds ionized in positive mode was based on a UHPLC HILIC column with 1.8 μm particle size, providing appropriate results for 22 out of the 24 species tested. In contrast, the detection of glyphosate and aminomethylphosphonic acid could only be achieved with a zwitterionic-type mixed-mode column, which proved to be suitable only for the pesticides detected in negative ion mode. Finally, the selected approach (UHPLC HILIC) was found to be useful for the determination of multiple pesticides in oranges using HILIC-ESI-MS/MS, with limits of quantitation in the low microgram per kilogram in most cases. Graphical Abstract HILIC improves separation of multiclass polar pesticides.

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Polar Reversed-Phase Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometer Method for Simple and Rapid Determination of Maleic Hydrazide Residues in some Fruits and Vegetables

et al. 2020

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Development of microwave-assisted extraction and liquid chromatography-tandem mass spectrometry for determination of maleic hydrazide residues in tobacco

Abstract: Herein, an effective and rapid method involving microwave-assisted extraction (MAE) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) was developed for the determination of maleic hydrazide residues in tobacco.

Cited by 4 publications

References 24 publications

Use of autoclave extraction and liquid chromatography with tandem mass spectrometry for determination of maleic hydrazide residues in tobacco

Use of autoclave extraction and liquid chromatography with tandem mass spectrometry for determination of maleic hydrazide residues in tobacco

Study of different HILIC, mixed-mode, and other aqueous normal-phase approaches for the liquid chromatography/mass spectrometry-based determination of challenging polar pesticides

Polar Reversed-Phase Liquid Chromatography Coupled with Triple Quadrupole Mass Spectrometer Method for Simple and Rapid Determination of Maleic Hydrazide Residues in some Fruits and Vegetables

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