Development of microsatellite primers for <i>Jatropha curcas</i> (Euphorbiaceae) and transferability to congeners

Bressan, Eduardo de Andrade; Scotton, Danielle Camargo; Ferreira, Renato Rodrigues; Jorge, Érika Cristina; Sebbenn, Alexandre Magno; Gerald, Lee Tseng Sheng; Figueira, Antônio

doi:10.3732/ajb.1100532

Cited by 21 publications

(14 citation statements)

References 8 publications

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“…A similarly low number of alleles was also documented by Sharma and Chauhan (2011), who found 2-6 alleles by transferring the SSR castor primer to jatropha. Similarly, Na-Ek et al (2011) observed 2-4 alleles per locus, and Bressan et al (2012) found 2-8 alleles per locus from new SSR markers developed for jatropha.…”

Section: Discussionmentioning

confidence: 91%

Population structure of jatropha and its implication for the breeding program

Santos¹,

Ferreira²,

Pasqual³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Jatropha (Jatropha curcas L.) has potential as an oilseed crop that requires the development of technology for its exploitation. The objective of this study was to assess the population structure and the genetic diversity in jatropha accessions at a global level using simple sequence repeat (SSR) molecular markers. Jatropha accessions (N = 109) from 10 countries were genotyped using 10 SSR markers. The results showed a low level of genetic diversity among 92 accessions originating from India,

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Section: Discussionmentioning

confidence: 91%

Population structure of jatropha and its implication for the breeding program

Santos¹,

Ferreira²,

Pasqual³

et al. 2016

Genet. Mol. Res.

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“…By analyzing Table 2, we see that studies performed to test the transferability between species of the same genus generally presented the highest rates. Bressan et al, (2012) observed a 67% transferability rate when they tested 9 pairs of primers designed for Jatropha curcas in Jatropha podagrica, Jatropha pohliana and Jatropha gossypifolia. Similarly, Sousa et al, (2009) obtained an average of 73% transferability from Centrosema pubercens primers in eleven species of the genus Centrosema.…”

Section: Resultsmentioning

confidence: 99%

Transferability of microsatellite loci from Croton floribundus Spreng. to Croton urucurana Baill. (Euphorbiaceae)

Motta¹,

Skowronski²,

Velasques³

et al. 2019

Afr. J. Biotechnol.

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Croton urucurana, known as "sangra-d'água", is a tree species with timber and beekeeping potential, and also with medicinal potential. The use of microsatellite markers, simple sequence repeats (SSRs) is efficient in population genetic studies, with high transferability rate between correlated species. The present study aimed to optimize the polymerase chain reaction (PCR) protocol and to test the transferability of seven primers developed for Croton floribundus to C. urucurana. The amplification of 113 accessions from different Brazilian biomes (Cerrado, Pantanal and Mata Atlântica) was tested. For optimization, the concentration and label of the MgCl 2 reagent was varied in addition to annealing temperature. Four tested primers showed amplification products, guaranteeing 57% of transferability. This result confirms the efficiency of C. floribundus microsatellite loci for future studies of genetic variability in C. urucurana.

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“…Five of these loci clearly displayed distinct banding patterns between 26 Mexican accessions (nontoxic) and the 10 Asian accessions (toxic). In the studies of Bressan et al [59], nine polymorphic microsatellite loci with 2-8 alleles per locus were identified, of which six loci showed transferability to three congeners: Jatropha podagrica, Jatropha pohliana and Jatropha gossypiifolia. Based on the whole genome sequences, Sato et al [77] identified about 41 000 SSR loci in the 289 Mb sequences of the J. curcas genome.…”

Section: Ssr Markersmentioning

confidence: 99%

Genetic diversity in the Jatropha genus and its potential application.

Xu¹,

Sujatha²,

Liu³

2012

CABI Reviews

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Jatropha curcas L., a drought tolerant, monoecious perennial shrub, has gained attention in the tropics and sub-tropics during the past decade as a potential biodiesel crop. Adequate genetic diversity for key agronomic traits is of fundamental importance in crop improvement programmes particularly for crops such as J. curcas, which are in the early stages of domestication. In J. curcas, genetic diversity in local populations and worldwide collections has been estimated using both dominant and co-dominant molecular markers systems such as random amplified polymorphic DNA (RAPD), inter simple sequence repeats (ISSRs), sequence-characterized amplified region (SCAR), amplified fragment length polymorphism (AFLP), simple sequence repeats (SSRs) and single-nucleotide polymorphism (SNPs), etc. Assessment of genetic variation using molecular markers unequivocally established the existence of rich genetic diversity in the germplasm from Central and South American regions and narrow genetic base in populations from Asia and Africa. Establishment of phylogenetic relationships among Jatropha species using molecular markers has been limited to species naturalized and distributed in India. Research expansion over the past decade has indicated the availability of considerable genetic variation in the genus Jatropha for vegetative and floral traits linked to productivity, seed oil content, fatty acid profiles, toxicity (phorbol esters, curcin), etc. The diverse genetic sources identified in J. curcas germplasm and the compatible wild species need to be exploited for genetic improvement of the crop through conventional breeding and interspecific gene transfer, which could be further accelerated through marker-aided selection.Review Methodology: The following were consulted for the primary literature review: CAB Abstracts, Agricola, Mendeley and ScienceDirect. Some of the articles were obtained using Google Scholar and Australian New Crops. The review presents an overview on the extent of genetic diversity available in Jatropha curcas germplasm and its wild allies as disclosed through different molecular marker systems and the role of genomics and marker-assisted breeding in genetic improvement of J. curcas.http://www.cabi.org/cabreviews CAB Reviews 2012 7, No. 059

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Development of microsatellite primers for Jatropha curcas (Euphorbiaceae) and transferability to congeners

Abstract: The primers developed here revealed polymorphic loci that are suitable for genetic diversity and structure, mating system, and gene flow studies in J. curcas, and some congeners.

Cited by 21 publications

References 8 publications

Population structure of jatropha and its implication for the breeding program

Population structure of jatropha and its implication for the breeding program

Transferability of microsatellite loci from Croton floribundus Spreng. to Croton urucurana Baill. (Euphorbiaceae)

Genetic diversity in the Jatropha genus and its potential application.

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