Development of microsatellite markers in <i>Capsella rubella</i> and <i>Capsella bursa‐pastoris</i> (Brassicaceae)

Caullet, Coraline; Pernin, Fanny; Poncet, Charles; Corre, Valérie Le

doi:10.3732/ajb.1100081

Cited by 3 publications

(3 citation statements)

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“…As stated above, C. bursa-pastoris is a species with a worldwide distribution, thus a priori facing very contrasted environments, which is expected to favour high phenotypic plasticity for traits associated to fitness ( Hendry 2016 ). In agreement with this expectation and previous studies ( Aksoy et al 1998 ; Caullet 2011 ; Neuffer 2011 ; Neuffer et al 2018 ), our experiment detected phenotypic plasticity for all traits ( Fig. 2 ): all accessions were able to survive in both common gardens, and all showed strong differences in phenotypes between the two common gardens.…”

Section: Discussionsupporting

confidence: 93%

The relative role of plasticity and demographic history in Capsella bursa-pastoris: a common garden experiment in Asia and Europe

et al. 2022

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Background and aims The colonization success of a species depends on the interplay between its phenotypic plasticity, adaptive potential and demographic history. Assessing their relative contributions during the different phases of a species range expansion is challenging, and requires large-scale experiments. Here, we investigated the relative contributions of plasticity, performance and demographic history to the worldwide expansion of the shepherd’s purse, Capsella bursa-pastoris. Methods We installed two large common gardens of the shepherd’s purse, a young, self-fertilizing, allopolyploid weed with a worldwide distribution. One common garden was located in Europe, the other in Asia. We used accessions from three distinct genetic clusters (Middle East, Europe, and Asia) that reflect the demographic history of the species. Several life-history traits were measured. To explain the phenotypic variation between and within genetic clusters, we analysed the effects of (i) the genetic clusters, (ii) the phenotypic plasticity and its association to fitness, and (iii) the distance in terms of bioclimatic variables between the sampling site of an accession and the common garden, i.e., the environmental distance. Key results Our experiment showed that (i) the performance of C. bursa-pastoris is closely related to its high phenotypic plasticity; (ii) within a common garden, genetic cluster was a main determinant of phenotypic differences; and (iii) at the scale of the experiment, the effect of environmental distance to the common garden could not be distinguished from that of genetic clusters. Conclusion Phenotypic plasticity and demographic history both play important role at different stages of range expansion. The success of the worldwide expansion of C. bursa-pastoris was undoubtedly influenced by its strong phenotypic plasticity.

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Section: Discussionsupporting

confidence: 93%

The relative role of plasticity and demographic history in Capsella bursa-pastoris: a common garden experiment in Asia and Europe

et al. 2022

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“…PCR amplifications were performed using a Mastercycler (Eppendorf, Hamburg, Germany) thermocycler, in a 20 μL reaction mix containing 70 mM Tris-HCL, 2 mM MgCl 2 , 17 mM (NH 4 ) 2 SO 4 , 10 mM beta-mercaptoethanol, 0.05% (wt/vol) polyoxyethylene-ether W1, 0.2 mg/mL bovine serum albumin, 200 mM of each dNTP, 10 ng genomic DNA, 0.5 units of Taq DNA polymerase, and 0.2 μM each of reverse and forward primers. The PCR program used consisted of 5 min at 95 °C, followed by 37 cycles of 5 s at 95 °C, 10 s at 60 °C and 30 s at 72 °C [21]. Amplicons were visualised under UV light by electrophoresis on a 3% (wt/vol) agarose gel stained with ethidium bromide.…”

Section: Methodsmentioning

confidence: 99%

Isolation and Characterisation of 11 Polymorphic Microsatellite Markers in Papaver rhoeas L. (Corn Poppy), a Major Annual Plant Species from Cultivated Areas

Kati

Corre

Michel

et al. 2012

IJMS

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Papaver rhoeas, an annual plant species in the Papaveraceae family, is part of the biodiversity of agricultural ecosystems and also a noxious agronomic weed. We developed microsatellite markers to study the genetic diversity of P. rhoeas, using an enriched microsatellite library coupled with 454 next-generation sequencing. A total of 13,825 sequences were obtained that yielded 1795 microsatellite loci. After discarding loci with less than six repeats of the microsatellite motif, automated primer design was successful for 598 loci. We tested 74 of these loci for amplification with a total of 97 primer pairs. Thirty loci passed our tests and were subsequently tested for polymorphism using 384 P. rhoeas plants originating from 12 populations from France. Of the 30 loci, 11 showed reliable polymorphism not affected by the presence of null alleles. The number of alleles and the expected heterozygosity ranged from 3 to 7.4 and from 0.27 to 0.73, respectively. A low but significant genetic differentiation among populations was observed (FST = 0.04; p < 0.001). The 11 validated polymorphic microsatellite markers developed in this work will be useful in studies of genetic diversity and population structure of P. rhoeas, assisting in designing management strategies for the control or the conservation of this species.

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“…[6] This genus is interesting because it is closely related to the Arabidopsis thaliana, a popular model organism in plant biology and genetics. [7] Only two species of the genus Capsella grow in Croatia, C. bursa-pastoris and C. rubella. [8] C. rubella is often replaced by mistake with C. bursa-pastoris, the most widespread Capsella specie throughout the world.…”

Section: Introductionmentioning

confidence: 99%

Volatile Constituents of Aerial Parts of Capsella rubella Reut.

Radonić¹,

Zekić²,

Marijanović³

2020

Croat. Chem. Acta

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Three different isolates from unexplored wild-growing Brassicaceae plant Capsella rubella were investigated in order to provide more complete insight of the composition and content of volatile constituents. The analysis of volatile compounds was performed by GC-FID/MS. C. rubella essential oil, that is isolate obtained by hydrodistillation, was characterized by a high percentage of 3,4-epithiobutanenitrile (67.8 %). The main volatile compounds in autolysate, i.e. isolate obtained by solvent extraction upon endogenous enzymatic hydrolysis, were 3,4epithiobutanenitrile (44.1 %) and ethyl isothiocyanate (29.4 %). Same as in autolysate, the main volatile constituents of hydrolysate, i.e. isolate obtained by solvent extraction upon exogenous enzymatic hydrolysis, were 3,4-epithiobutanenitrile and ethyl isothiocyanate, but with ethyl isothiocyanate as the dominating compound (57.8 %) followed by 3,4-epithiobutanenitrile (15.6 %). The results showed that qualitative and quantitative composition of C. rubella volatile constituents depends on the isolation method. But, regardless of the isolation method sulfurand nitrogen-containing volatile compounds were quantitatively dominating constituents in all isolates.

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Development of microsatellite markers in Capsella rubella and Capsella bursa‐pastoris (Brassicaceae)

Abstract: The results provide valuable information on genetic diversity for future studies of population genetics in C. rubella and C. bursa-pastoris.

Cited by 3 publications

References 9 publications

The relative role of plasticity and demographic history in Capsella bursa-pastoris: a common garden experiment in Asia and Europe

The relative role of plasticity and demographic history in Capsella bursa-pastoris: a common garden experiment in Asia and Europe

Isolation and Characterisation of 11 Polymorphic Microsatellite Markers in Papaver rhoeas L. (Corn Poppy), a Major Annual Plant Species from Cultivated Areas

Volatile Constituents of Aerial Parts of Capsella rubella Reut.

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