Development of microsatellite markers for a diving duck, the common pochard (Aythya ferina)

Šťovíček, Ondřej; Čížková, Dagmar; Liu, Yang; Albrecht, Tomáš; Heckel, Gerald; Vyskočilová, Martina; Kreisinger, Jakub

doi:10.1007/s12686-011-9407-6

Cited by 3 publications

(1 citation statement)

References 12 publications

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“…The microsatellites were amplified using polymerase chain reaction (PCR) on a 10 μl reaction mixture consisting of 30–60 ng DNA, 4 μl of PCR master mix and 1 μl of primer mixture. PCR conditions and further details are as described in Šťovíček et al (2011). PCR products were sized using an automated capillary sequencer with LIZ 500 as the standard.…”

Section: Methodsmentioning

confidence: 99%

High rates of conspecific brood parasitism revealed by microsatellite analysis in a diving duck, the common pochard Aythya ferina

Šťovíček¹,

Kreisinger²,

Javůrková³

et al. 2013

Journal of Avian Biology

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Conspecific brood parasitism (CBP) is a reproductive tactic whereby a parasitic female lays its eggs into the nests of other conspecific females. Genetic‐based data on the occurrence of CBP in birds, however, is still relatively scarce. We analysed prevalence of CBP in a ground‐nesting diving duck, the common pochard Aythya ferina, using a set of 17 microsatellites. Compared to related species, our population showed a relatively high level of CBP, with 39% of genotyped pochard eggs laid parasitically and 89% of nests containing ≥ 1 parasitic egg. In addition, we observed relatively high rates of interspecific brood parasitism (13% of eggs), caused predominantly by mallard Anas plathyrhynchos and tufted duck Aythya fuligula. CBP eggs had decreased hatching success compared to host eggs, with 65% of CBP and 95% of non‐CBP genotyped eggs hatching successfully. Our data suggest that this was probably due to improper timing of parasitic egglaying, which compromised synchronised hatching of CBP and host‐eggs. Despite high rates of CBP in our pochard popu lation, fitness costs associated with this reproductive tactic appear to be low for host females since neither clutch size nor host‐egg hatching probability were reduced due to CBP.

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Section: Methodsmentioning

confidence: 99%

High rates of conspecific brood parasitism revealed by microsatellite analysis in a diving duck, the common pochard Aythya ferina

Šťovíček¹,

Kreisinger²,

Javůrková³

et al. 2013

Journal of Avian Biology

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Breeding site fidelity and winter admixture in a long-distance migrant, the tufted duck (Aythya fuligula)

2012

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Long-distance migrants are, by definition, highly mobile but it is poorly understood if this leads to high rates of gene flow and an essentially panmictic global population structure. Genetic divergence in migratory species could be promoted, for example, by fidelity to distinct migratory pathways. In this study, we investigate the population genetic structure of tufted duck (Aythya fuligula), a long-distance migrant with a largely continuous breeding distribution across Eurasia. Distinct, longitudinally oriented flyways have been postulated based on geographically disjunct wintering areas and are supported by evidence from ringing data. We generated sequences of the mitochondrial control region and multi-locus microsatellite genotypes for several hundreds of samples from the European and Asian breeding and wintering grounds including some individuals infected with highly pathogenic avian influenza virus H5N1. Significant differentiation between breeding sites was observed for both marker types, but F ST values were approximately 10 times higher for maternally inherited mitochondrial DNA than for biparentally transmitted nuclear markers. The genetic differentiation between the postulated European and Asian flyways was similar to that observed within continents and, in general, genetic divergence was not associated with geographic distance. Neither marker type showed evidence of genetic substructure among aggregations on the European wintering grounds. Our results suggest some breeding site fidelity, especially in females, but extensive population admixture on the wintering grounds. Several scenarios may explain the observed lack of genetic divergence between Europe and Asia including non-equilibrium conditions following a recent range expansion or contemporary gene flow across the postulated migratory divides.

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Temporal genetic structure and relatedness in the Tufted DuckAythya fuligulasuggests limited kin association in winter

Liu¹,

Keller²,

Heckel³

2013

Ibis

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Conspecific aggregation of waterfowl in winter is a common example of animal flocking behaviour, yet patterns of relatedness and temporal substructure in such social groups remain poorly understood even in common species. A previous study based on mark‐recapture data showed that Tufted Ducks Aythya fuligula caught on the same day were re‐caught together in subsequent winters more often than expected by chance, suggesting stable assortments of ‘socially familiar’ individuals between wintering periods. The genetic relationships within these social groups were not clear. Based on 191 individuals genotyped at 10 microsatellite markers, we investigated the temporal genetic structure and patterns of relatedness among wintering Tufted Ducks at Lake Sempach, Switzerland, in two consecutive winters. We found no evidence of genetic differentiation between temporal groups within or between winters. The average levels of relatedness in temporal groups were low and not higher than expected in random assortments of individuals. However, Mantel tests performed for each sex separately revealed significant negative correlations between the pairwise relatedness coefficients and the number of days between the capture dates of pairs of wintering Tufted Duck in males and females. This pattern suggests the presence of a small number of co‐migrating same‐sex sibling pairs in wintering flocks of Tufted Ducks. Our findings provide one of the first genetic analyses of a common duck species outside the breeding season and contribute to the understanding of social interactions in long‐distance migratory birds.

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Development of microsatellite markers for a diving duck, the common pochard (Aythya ferina)

Cited by 3 publications

References 12 publications

High rates of conspecific brood parasitism revealed by microsatellite analysis in a diving duck, the common pochard Aythya ferina

High rates of conspecific brood parasitism revealed by microsatellite analysis in a diving duck, the common pochard Aythya ferina

Breeding site fidelity and winter admixture in a long-distance migrant, the tufted duck (Aythya fuligula)

Temporal genetic structure and relatedness in the Tufted DuckAythya fuligulasuggests limited kin association in winter

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