Development of microsatellite markers for the resin‐yielding, non‐timber forest product species Boswellia serrata (Burseraceae)

Abstract

Premise of the Study Boswellia serrata (Burseraceae) is an economically important aromatic, gum‐resin–yielding, non‐timber forest tree species. Microsatellite markers were developed for B. serrata for the first time to study genetic diversity and population structure.Methods and ResultsA magnetic bead enrichment method was used to develop 16 microsatellite markers, of which 11 were polymorphic. The number of alleles per locus in the 60 individuals studied ranged from three to 10, and the levels of observed and expected heterozygosity ranged from 0.50 to 0.90 and 0.666 to 0.861, respectively. The primers successfully amplified in the congeneric species B. ovalifoliolata.ConclusionsThese microsatellite markers can be used to study the genetic variation and population structure of B. serrata and to provide crucial information on population and ecological issues for management and conservation of the species.

Population Genetics, Genetic Structure, and Inbreeding of Commiphora gileadensis (L.) C. Chr Inferred from SSR Markers in Some Mountainous Sites of Makkah Province

“…In this study, all the genetic diversity variables of C. gileadensis showed a modest to extreme decline in measurements among all the enduring populations analysed and was in agreement with other studies on closely related plant species of Burseraceae. This indicates an extensive decline in gene diversity and meets the corresponding severe environmental circumstances [28][29][30][31][32][33][34] confirmed by the comparison of the high genetic differentiation F ST values, which measured for C. gileadensis (F ST = 0.896) with other rare plant species in similar plant habitats in South Sinai, and they revealed considerable values of genetic differentiation, e.g., Primula boveana (F ST = 0.737) [35] and Cotoneaster orbicularis (F ST = 0.634) [32].…”

“…Isolation of DNA from the preserved leaflet samples for 600 plant individuals was performed using a DNeasy Plant Mini Kit (Qiagen, Germantown, MD, USA). Fifty loci revealing polymorphisms were recognized using sixty-one formerly published primers for other species belonging to the Burseraceae family [28][29][30][31]. The polymorphic primer tests were performed for all sampled individuals (Table S1).…”

Development and Application of Potentially Universal Microsatellite Markers for Pheasant Species

“…Novel microsatellite markers need to be developed for other pheasant birds. Before genome sequencing was widely used, microsatellite markers were mainly developed by using a magnetic bead enrichment method, enriched genomic libraries and cross-species screening of closely related species to screen [25][26][27], which was inefficient and time-consuming. Many microsatellite sequences can be obtained by directly sequencing the genome and rapidly screening from the genome using Next Generation Sequencing (NGS) technology (Illumina MiSeq v3) and bioinformatics software [28,29].…”