Development of Microsatellite Markers by ISSR-suppression-PCR Method in Brassica rapa

Tamura, Koji; Nishioka, Miki; Zhang, Zengcui; Chen, Lian; Hougetsu, Taizo; Harada, Kyuya

doi:10.1270/jsbbs.55.247

Cited by 16 publications

(15 citation statements)

References 30 publications

(32 reference statements)

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“…These 33 IN, 25 SS, and 21 WS lines were genotyped, respectively, with 32, 14, and 36 polymorphic simple sequence repeat (SSR) markers, which were selected from 191 markers through screening of the parents for polymorphism. These markers were collected from various sources: Agriculture and Agri-Food Canada (AAFC) through a material transfer agreement (currently available in http://aafc-aac.usask.ca/BrassicaMAST/), and the markers published by Lowe et al (2004), Piquemal et al (2005), Tamura et al (2005), Iniguez-Luy et al (2008), and Cheng et al (2009). Genotyping of the lines was done following the method described in Bennett et al (2012) and Kebede et al (2010).…”

Section: Population Developmentmentioning

confidence: 99%

Patterns of Heterosis in Three Distinct Inbred Populations of Spring Brassica napus Canola

2016

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Allelic diversity of the allied species of Brassica napus L. as well as of the winter form of this species has been demonstrated to be related with increasing productivity of hybrid spring B. napus cultivars. To compare potential value of the different gene pools of Brassica species three spring B. napus inbred populations were developed by use of a B. oleracea L. line, a spring B. napus breeding line, and a winter B. napus cultivar crossed to a spring B. napus ‘Hi‐Q’; and test hybrids of these inbred lines were produced by crossing with Hi‐Q as the common tester. Mid‐parent heterosis (MPH) showed a negative correlation with seed yield of the inbred lines in all three populations; however, a positive correlation existed between seed yield of the inbred lines and heterosis over Hi‐Q (HiQH) (or, inbred vs. hybrid yield). On average, the level of MPH in hybrid of the inbred lines derived from B. napus × B. oleracea cross was twice greater than the level of heterosis found for the inbred lines derived from spring × spring or winter × spring B. napus crosses. The inbred population derived from winter × spring cross gave highest seed yield, and this population also gave highest HiQH. The results suggested that B. oleracea and winter canola could be used in spring B. napus canola breeding for accumulating additive and non‐additive effect genes for increased seed yield in hybrid cultivars.

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Section: Population Developmentmentioning

confidence: 99%

Patterns of Heterosis in Three Distinct Inbred Populations of Spring Brassica napus Canola

2016

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“…The objectives of future studies are the conversion of AFLP markers that are tightly linked to the bolting time locus into STS markers and mapping of SSRs which were isolated from the B. rapa genome (Tamura et al 2005). Integration of SSR markers into the present linkage map could be useful for MAS for bolting time in breeding programs of Brassica plants.…”

Section: Qtl Analysis For Bolting Timementioning

confidence: 99%

Mapping of QTLs for Bolting Time in Brassica rapa (syn. campestris) under Different Environmental Conditions

et al. 2005

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The bolting time of varieties used as leafy vegetables or turnips within the Brassica rapa (syn. campestris) species is important, because it affects the yield and quality of the harvested products. In Brassica rapa, the transition from the vegetative to reproductive phase is influenced by environmental factors, such as daylength and low temperature. In order to analyze the genetic basis of bolting time, an AFLP linkage map was constructed using doubled haploid (DH) lines derived from the F 1 hybrid between A9408 and Homei P09. QTL analysis of bolting time was performed based on the evaluation under two field conditions and three artificial conditions differing in day-length and/or temperature. Out of a total of ten QTLs detected, two QTLs showed a low temperature sensitivity. The most effective QTL, BT1, was found to be the main locus controlling the vernalization response and the allele of Homei P09 was highly responsive to low temperature compared with that of the late parent A9408. These results could be useful for marker-assisted selection and isolation of the genes responsible for bolting time in Brassica rapa.

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“…Of these, AccII was found to be the most useful for P. helicoides, because c. 90% of the sequenced clones from this library contained microsatellites. Tamura et al (2005) tested the same six restriction enzymes as those used in this study in Brassica rapa, and obtained better results with EcoRV and SspI. These observations suggest that the optimum restriction enzymes for cloning microsatellites might be different among taxonomic groups.…”

Section: Discussionmentioning

confidence: 60%

“…Tamura et al (2005) tested the same six restriction enzymes as those used in this study in Brassica rapa, and obtained better results with EcoRV and SspI. In general, we obtained larger numbers of useful microsatellite clones from the libraries developed using the four-base recognition enzymes.…”

Section: Discussionmentioning

confidence: 81%

Development of microsatellite markers forPythium helicoides

Yin-Ling

Zhou

Motohashi

et al. 2009

FEMS Microbiology Letters

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A strategy combining dual-suppression PCR and thermal asymmetric interlaced PCR was used to determine sequences flanking microsatellite regions in Pythium helicoides. The primer pairs were designed to amplify loci containing (AC)n, (GA)n, (AGC)n, (CAC)n(CAA)n, (TCA)n and (CTTT)n repeats from the P. helicoides nuclear genome. The PCR products of each primer pair, amplified from three representative isolates collected from different hosts and locations, were cloned and sequenced. Different degrees of polymorphism were detected among these microsatellite markers. The numbers of alleles were 6, 2, 4, 11, 4 and 4 in YL-AC, YL-AGC, YL-CAA, YL-CTTT, YL-GA and YL-TCA, respectively. Allele analysis of 30 P. helicoides isolates showed length polymorphisms in all loci, except for YL-AC, using capillary electrophoresis. Thus, we have developed a simple method for designing PCR primers to amplify microsatellite markers from P. helicoides.

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Development of Microsatellite Markers by ISSR-suppression-PCR Method in Brassica rapa

Cited by 16 publications

References 30 publications

Patterns of Heterosis in Three Distinct Inbred Populations of Spring Brassica napus Canola

Patterns of Heterosis in Three Distinct Inbred Populations of Spring Brassica napus Canola

Mapping of QTLs for Bolting Time in Brassica rapa (syn. campestris) under Different Environmental Conditions

Development of microsatellite markers forPythium helicoides

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