Development of microsatellite loci for Taxus wallichiana var. wallichiana (Taxaceae) and cross-amplification in Taxaceae

Abstract

ABSTRACT. Nine polymorphic microsatellite loci were isolated and characterized in Taxus wallichiana var. wallichiana, an endangered species in China. The number of alleles per locus ranged from 2 to 20. Observed and expected heterozygosities varied from 0.0260 to 0.5325 and 0.3603 to 0.9231, respectively. Positive cross-amplification of the 9 loci was observed in 2 other varieties of T. wallichiana and 4 other Taxaceae species. These loci will be of value for studying population genetic structures and for genetic resource conservation in T. wallichiana and other Taxus species.

Development of polymorphic microsatellite markers for Japanese yew, Taxus cuspidata, and T. cuspidata var. nana (Taxaceae)

“… Ueno et al (2015) developed 80 EST-SSR markers for T. cuspidata ; however, levels of diversity are often lower in EST-SSRs than in genomic SSRs. Although genomic SSR markers were developed in some relatives of T. cuspidata (e.g., Dubreuil et al, 2008 ; Cheng et al, 2015a ), their utility for T. cuspidata was limited and the level of polymorphism in T. cuspidata populations was low ( Cheng et al, 2015b ). Therefore, we have developed highly polymorphic, genomic microsatellite markers to investigate the spatial genetic structure of T .…”