Development of microsatellite loci and population genetics in the bumblebee catfish species Pseudopimelodus atricaudus and Pseudopimelodus magnus (Siluriformes: Pseudopimelodidae)

Abstract

The Neotropical freshwater catfish Pseudopimelodus atricaudus and Pseudopimelodus magnus are two recently discovered species endemic to the Colombian Magdalena-Cauca River basin. In this study, a set of 13 microsatellite loci were developed by using next generation sequence technology to assess the genetic diversity and population structure in P. atricaudus and test for cross-species amplification in P. magnus. Both species exhibited high genetic diversity (P. atricaudus: Na: 9.000 - 9.769 alleles/locus, Ho: 0.760 - 0.804, HE: 0.804 - 0.840; P. magnus: Na: 12.8 - 5.4 alleles/locus, Ho: 0.638 - 0.683, HE: 0.747 - 0.755) compared to the mean levels of genetic diversity reported for Neotropical Siluriformes, and lack of genetic differentiation among sampling sites within the Cauca River (P. atricaudus: F’ST=0.013 - 0.017, P > 0.05, D’est= -0.004 - 0.023, P > 0.05; P. magnus: F’ST= 0.031, P= 0.055; D’est= 0.045, P= 0.058). This work is the first insight on the diversity and the population genetics of species of the family Pseudopimelodidae and provides a framework to further population genetic and conservation analyses needed in this poorly studied family at the microevolutionary level.

Genetic insights into Cyphocharax magdalenae (Characiformes: Curimatidae): Microsatellite loci development and population analysis in the Cauca River, Colombia

“…Additionally, this study corroborated the hypothesis of high gene flow despite having found significant differences among individuals of the lower section of the Cauca River as those differences are related to the coexistence of two genetic stocks with uneven distribution through the river. This indicates that there is no evidence of barriers separating the gene flow of C. magdalenae in the lower section of the Cauca River, which is consistent with data reported for C. mivartii [23], P. magdalenae [26], Cynopotamus magdalenae (Steindachner 1879), Megaleporinus muyscorum [85] and catfishes A. pardalis, P. grosskopfii, S. cuspicaudus [86], P. magdaleniatum [30], P. atricaudus and P. magnus [36]. Nonetheless, genetic structure resulting from…”

“…The scarce information for C. magdalenae contrasts with population genetic studies performed for Curimata mivartii [23] and species with fishing relevance of the phylogenetically close family Prochilodontidae like Prochilodus magdalenae [24][25][26], Prochilodus reticulatus [27] and Ichthyoelephas longirostris [28]. Likewise, it contrasts with studies on species of other families inhabiting the Magdalena-Cauca basin such as Pseudoplatystoma magdaleniatum [29,30], Brycon henni [31][32][33], S. cuspicaudus, Ageneiosus pardalis, Pimelodus grosskopfii [34], Pimelodus yuma [35], Pseudopimelodus magnus and Pseudopimelodus atricaudus [36]. Among the different markers used in the cited studies, microsatellites stand out as the most used tool to solve questions at micro-evolutive scale in population genetics.…”

“…atricaudus and P . magnus [ 36 ]. Nonetheless, genetic structure resulting from seasonal variation was found since comparisons over the samples in rainy and dry seasons during the assessed years allowed detecting that one stock predominates in the dry season (Stock1) while the other predominates in the rainy season (Stock2).…”

“…Likewise, it contrasts with studies on species of other families inhabiting the Magdalena-Cauca basin such as Pseudoplatystoma magdaleniatum [ 29 , 30 ], Brycon henni [ 31 – 33 ], S . cuspicaudus , Ageneiosus pardalis , Pimelodus grosskopfii [ 34 ], Pimelodus yuma [ 35 ], Pseudopimelodus magnus and Pseudopimelodus atricaudus [ 36 ]. Among the different markers used in the cited studies, microsatellites stand out as the most used tool to solve questions at micro-evolutive scale in population genetics.…”

Spatial population genetic structure of Caquetaia kraussii (Steindachner, 1878) evidenced by species-specific microsatellite loci in the middle and low basin of the Cauca River, Colombia

“…The impact of these factors on the population genetics of C. kraussii remains unknown due to the lack of previous information on these subjects. However, the significant gene flow observed in various migratory fish species coexisting in this area [48][49][50][121][122][123] suggests the absence of physical or chemical barriers limiting gene flow in these species.…”